Strain identifier

BacDive ID: 24805

Type strain: Yes

Species: Borrelia sinica

Strain Designation: CMN3

Strain history: T. Masuzawa CMN3.

NCBI tax ID(s): 87162 (species)

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General

@ref: 20464

BacDive-ID: 24805

DSM-Number: 23262

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, animal pathogen

description: Borrelia sinica CMN3 is a microaerophile, mesophilic animal pathogen that was isolated from Niviventer confucianus.

NCBI tax id

  • NCBI tax id: 87162
  • Matching level: species

strain history

@refhistory
20464<- T. Masuzawa, Chiba Inst. Sci., Choshi, Japan; CMN3 <- N. Takada, Fukui Medical Univ., Japan
67770T. Masuzawa CMN3.

doi: 10.13145/bacdive24805.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Spirochaetales
  • family: Borreliaceae
  • genus: Borrelia
  • species: Borrelia sinica
  • full scientific name: Borrelia sinica Masuzawa et al. 2001
  • synonyms

    • @ref: 20215
    • synonym: Borreliella sinica

@ref: 20464

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Borreliaceae

genus: Borrelia

species: Borrelia sinica

full scientific name: Borrelia sinica Masuzawa et al. 2001

strain designation: CMN3

type strain: yes

Morphology

colony morphology

  • @ref: 20464
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 20464
  • name: BSK-MEDIUM (DSMZ Medium 403)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/403
  • composition: Name: BSK-MEDIUM (DSMZ Medium 403) Composition: Bovine serum albumin 35.0245 g/l Gelatine 9.79706 g/l HEPES buffer 4.19874 g/l Proteose peptone no. 2 3.49895 g/l Glucose 2.09937 g/l Proteose yeastolate 0.69979 g/l Tryptone peptone 0.69979 g/l Na-pyruvate 0.559832 g/l Sodium citrate 0.489853 g/l N-Acetylglucosamine 0.279916 g/l MgCl2 x 6 H2O 0.209937 g/l L-Glutamine 0.069979 g/l Double distilled water CMRL 1066 Rabbit serum

culture temp

@refgrowthtypetemperaturerange
20464positivegrowth30mesophilic
67770positivegrowth34mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 20464
  • oxygen tolerance: microaerophile

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
20464Niviventer confucianusNiviventer confucianusSichuan province, NanchuanChinaCHNAsia
67770Niviventer confuciamus captured in Sichuan ProvinceChinaCHNAsia

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Muridae (Mouse/Rat)

Safety information

risk assessment

  • @ref: 20464
  • pathogenicity animal: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20464
  • description: Borrelia sinica gene for 16S rRNA, partial sequence, strain: CMN3
  • accession: AB022101
  • length: 1502
  • database: ena
  • NCBI tax ID: 87162

GC content

  • @ref: 20464
  • GC-content: 29.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 20464

culture collection no.: DSM 23262, JCM 10505

straininfo link

  • @ref: 88627
  • straininfo: 402129

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20464Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-23262Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23262)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
88627Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402129.1