Strain identifier
BacDive ID: 24802
Type strain:
Species: Pelolinea submarina
Strain Designation: MO-CFX1, MO-CFXI
Strain history: <- Sanae Sakai, JAMSTEC
NCBI tax ID(s): 913107 (species)
General
@ref: 21321
BacDive-ID: 24802
DSM-Number: 23923
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, filament-shaped
description: Pelolinea submarina MO-CFX1 is an anaerobe, mesophilic, filament-shaped bacterium that was isolated from marine subsurface sediment.
NCBI tax id
- NCBI tax id: 913107
- Matching level: species
strain history
@ref | history |
---|---|
21321 | <- S. Sakai, Japan Agency for Marine-Earth Sci. and Technol. (JAMSTEC), Yokosuka; MO-CFXI <- H. Imachi |
67770 | S. Sakai MO-CFXI. |
67771 | <- Sanae Sakai, JAMSTEC |
doi: 10.13145/bacdive24802.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/chloroflexota
- domain: Bacteria
- phylum: Chloroflexota
- class: Anaerolineae
- order: Anaerolineales
- family: Anaerolineaceae
- genus: Pelolinea
- species: Pelolinea submarina
- full scientific name: Pelolinea submarina Imachi et al. 2014
@ref: 21321
domain: Bacteria
phylum: Chloroflexi
class: Anaerolineae
order: Anaerolineae, not assigned to order
family: Anaerolineaceae
genus: Pelolinea
species: Pelolinea submarina
full scientific name: Pelolinea submarina Imachi et al. 2014
strain designation: MO-CFX1, MO-CFXI
type strain: yes
Morphology
cell morphology
@ref | cell shape | motility | gram stain | confidence |
---|---|---|---|---|
67771 | filament-shaped | no | ||
67771 | negative | |||
69480 | negative | 98.881 |
Culture and growth conditions
culture medium
- @ref: 21321
- name: MO-CFXI MEDIUM (DSMZ Medium 1378)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1378
- composition: Name: MO-CFXI MEDIUM (DSMZ Medium 1378) Composition: NaCl 25.0 g/l MgCl2 x 6 H2O 4.0 g/l NaHCO3 2.0 g/l Glucose 1.8 g/l CaCl2 x 2 H2O 1.0 g/l Tryptone 1.0 g/l Yeast extract 1.0 g/l Sodium pyruvate 0.55 g/l NH4Cl 0.535 g/l KCl 0.5 g/l Na2S 0.3 g/l Cysteine-HCl 0.3 g/l KH2PO4 0.1 g/l FeCl2 x 4 H2O 0.00127 g/l MnCl2 x 4 H2O 0.0002 g/l ZnCl2 0.00014 g/l CoCl2 x 6 H2O 0.00013 g/l Na2MoO4 x 2 H2O 2e-05 g/l D-Calcium pantothenate 1.9e-05 g/l Folic acid 1.76e-05 g/l Riboflavin 1.5e-05 g/l Thiamine-HCl x 2 H2O 1.34e-05 g/l NiCl2 1e-05 g/l AlCl3 1e-05 g/l Biotin 9.8e-06 g/l Lipoic acid 8.2e-06 g/l Pyridoxine hydrochloride 8.2e-06 g/l H3BO3 6e-06 g/l p-Aminobenzoic acid 5.4e-06 g/l Nicotinic acid 4.8e-06 g/l Na2WO4 x 2 H2O 3e-06 g/l Na2SeO4 2e-06 g/l CuCl2 x 2 H2O 1e-06 g/l Vitamin B12 2e-07 g/l Resazurin Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
21321 | positive | growth | 25 | mesophilic |
67770 | positive | growth | 25 | mesophilic |
67771 | positive | growth | 25 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
67771 | anaerobe | |
69480 | anaerobe | 99.384 |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.97 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
---|---|---|---|---|---|---|---|
21321 | marine subsurface sediment | off the Shimokita Peninsula, north-western Pacific Ocean (Site C9001, 41° 10.6389' N 142° 12.081' E, 1180 m water depth) | Japan | JPN | Asia | 41.1773 | 142.201 |
67770 | Marine subsurface sediments collected off the Shimokita Peninsula of Japan | north-western Pacific Ocean | Pacific Ocean | ||||
67771 | From marine subsurface sediments collected off the Shimokita Peninsula of Japan | north-western Pacific Ocean (Site C9001) | Japan | JPN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Environmental | #Aquatic | #Sediment |
taxonmaps
- @ref: 69479
- File name: preview.99_82729.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_925;96_14293;97_43930;98_57446;99_82729&stattab=map
- Last taxonomy: Pelolinea submarina subclade
- 16S sequence: AB598277
- Sequence Identity:
- Total samples: 823
- soil counts: 91
- aquatic counts: 680
- animal counts: 37
- plant counts: 15
Sequence information
16S sequences
- @ref: 21321
- description: Pelolinea submarina gene for 16S rRNA, partial sequence
- accession: AB598277
- length: 1432
- database: ena
- NCBI tax ID: 913107
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pelolinea submarina MO-CFX1 | GCA_003966975 | complete | ncbi | 913107 |
66792 | Pelolinea submarina DSM 23923 | GCA_003385075 | scaffold | ncbi | 913107 |
66792 | Pelolinea submarina strain DSM 23923 | 913107.6 | wgs | patric | 913107 |
66792 | Pelolinea submarina DSM 23923 | 2770939606 | draft | img | 913107 |
GC content
- @ref: 21321
- GC-content: 52.4
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 89.735 | no |
flagellated | no | 96.739 | yes |
gram-positive | no | 88.975 | no |
anaerobic | yes | 95.214 | yes |
aerobic | no | 96.679 | yes |
halophile | no | 91.251 | no |
spore-forming | no | 92.668 | no |
thermophile | yes | 79.324 | yes |
glucose-util | yes | 87.68 | no |
glucose-ferment | no | 59.143 | no |
External links
@ref: 21321
culture collection no.: DSM 23923, JCM 17238, KCTC 5975
straininfo link
- @ref: 88624
- straininfo: 396993
literature
- topic: Phylogeny
- Pubmed-ID: 24215824
- title: Pelolinea submarina gen. nov., sp. nov., an anaerobic, filamentous bacterium of the phylum Chloroflexi isolated from subseafloor sediment.
- authors: Imachi H, Sakai S, Lipp JS, Miyazaki M, Saito Y, Yamanaka Y, Hinrichs KU, Inagaki F, Takai K
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.057547-0
- year: 2013
- mesh: Bacterial Typing Techniques, Base Composition, Chloroflexi/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Japan, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Water Microbiology
- topic2: Genetics
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
21321 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23923 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23923) | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
88624 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID396993.1 |