Workshop and Q&A October 6th

Strain identifier

BacDive ID: 24802

Type strain: Yes

Species: Pelolinea submarina

Strain Designation: MO-CFX1, MO-CFXI

Strain history: <- Sanae Sakai, JAMSTEC

NCBI tax ID(s): 913107 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 7 (current version):
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 7 (current version)

General

@ref: 21321

BacDive-ID: 24802

DSM-Number: 23923

keywords: filament-shaped, mesophilic, anaerobe, Bacteria, 16S sequence, genome sequence

description: Pelolinea submarina MO-CFX1 is an anaerobe, mesophilic, filament-shaped bacterium that was isolated from marine subsurface sediment.

NCBI tax id

  • NCBI tax id: 913107
  • Matching level: species

strain history

doi: 10.13145/bacdive24802.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/chloroflexota
  • domain: Bacteria
  • phylum: Chloroflexota
  • class: Anaerolineae
  • order: Anaerolineales
  • family: Anaerolineaceae
  • genus: Pelolinea
  • species: Pelolinea submarina
  • full scientific name: Pelolinea submarina Imachi et al. 2014

@ref: 21321

domain: Bacteria

phylum: Chloroflexi

class: Anaerolineae

order: Anaerolineae, not assigned to order

family: Anaerolineaceae

genus: Pelolinea

species: Pelolinea submarina

full scientific name: Pelolinea submarina Imachi et al. 2014

strain designation: MO-CFX1, MO-CFXI

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stain
67771filament-shapedno
67771negative

Culture and growth conditions

culture medium

  • @ref: 21321
  • name: MO-CFXI MEDIUM (DSMZ Medium 1378)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/1378
  • composition: Name: MO-CFXI MEDIUM (DSMZ Medium 1378) Composition: NaCl 25.0 g/l MgCl2 x 6 H2O 4.0 g/l NaHCO3 2.0 g/l Glucose 1.8 g/l Tryptone 1.0 g/l Yeast extract 1.0 g/l CaCl2 x 2 H2O 1.0 g/l Sodium pyruvate 0.55 g/l NH4Cl 0.535 g/l KCl 0.5 g/l Cysteine-HCl 0.3 g/l Na2S 0.3 g/l KH2PO4 0.1 g/l FeCl2 x 4 H2O 0.00127 g/l MnCl2 x 4 H2O 0.0002 g/l ZnCl2 0.00014 g/l CoCl2 x 6 H2O 0.00013 g/l Na2MoO4 x 2 H2O 2e-05 g/l D-Calcium pantothenate 1.9e-05 g/l Folic acid 1.76e-05 g/l Riboflavin 1.5e-05 g/l Thiamine-HCl x 2 H2O 1.34e-05 g/l NiCl2 1e-05 g/l AlCl3 1e-05 g/l Biotin 9.8e-06 g/l Lipoic acid 8.2e-06 g/l Pyridoxine hydrochloride 8.2e-06 g/l H3BO3 6e-06 g/l p-Aminobenzoic acid 5.4e-06 g/l Nicotinic acid 4.8e-06 g/l Na2WO4 x 2 H2O 3e-06 g/l Na2SeO4 2e-06 g/l CuCl2 x 2 H2O 1e-06 g/l Vitamin B12 2e-07 g/l Resazurin Distilled water

culture temp

@refgrowthtypetemperaturerange
21321positivegrowth25mesophilic
67770positivegrowth25mesophilic
67771positivegrowth25mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
21321marine subsurface sedimentoff the Shimokita Peninsula, north-western Pacific Ocean (Site C9001, 41° 10.6389' N 142° 12.081' E, 1180 m water depth)JapanJPNAsia41.1773142.201
67770Marine subsurface sediments collected off the Shimokita Peninsula of Japannorth-western Pacific OceanPacific Ocean
67771From marine subsurface sediments collected off the Shimokita Peninsula of Japannorth-western Pacific Ocean (Site C9001)JapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

Sequence information

16S sequences

  • @ref: 21321
  • description: Pelolinea submarina gene for 16S rRNA, partial sequence
  • accession: AB598277
  • length: 1432
  • database: ena
  • NCBI tax ID: 913107

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pelolinea submarina MO-CFX1GCA_003966975completencbi913107
66792Pelolinea submarina DSM 23923GCA_003385075scaffoldncbi913107
66792Pelolinea submarina strain DSM 23923913107.6wgspatric913107
66792Pelolinea submarina DSM 239232770939606draftimg913107
67770Pelolinea submarina MO-CFX1 DNA, complete genomeAP018437ena913107

GC content

  • @ref: 21321
  • GC-content: 52.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 21321

culture collection no.: DSM 23923, JCM 17238, KCTC 5975

straininfo link

  • @ref: 20218
  • passport: http://www.straininfo.net/strains/894358

literature

  • topic: Phylogeny
  • Pubmed-ID: 24215824
  • title: Pelolinea submarina gen. nov., sp. nov., an anaerobic, filamentous bacterium of the phylum Chloroflexi isolated from subseafloor sediment.
  • authors: Imachi H, Sakai S, Lipp JS, Miyazaki M, Saito Y, Yamanaka Y, Hinrichs KU, Inagaki F, Takai K
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.057547-0
  • year: 2013
  • mesh: Bacterial Typing Techniques, Base Composition, Chloroflexi/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Japan, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Water Microbiology
  • topic2: Genetics

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.StrainInfo introduces electronic passports for microorganisms.10.1016/j.syapm.2013.11.002Syst Appl Microbiol. 37: 42-50 201424321274
21321Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-23923Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23923)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc