Strain identifier

BacDive ID: 24769

Type strain: Yes

Species: Blautia faecis

Strain Designation: M25

Strain history: <- Jin-Woo Bae, Kyung Hee Univ.

NCBI tax ID(s): 871665 (species)

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General

@ref: 21179

BacDive-ID: 24769

DSM-Number: 27629

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, ovoid-shaped

description: Blautia faecis M25 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from human faeces, healthy Korean male.

NCBI tax id

  • NCBI tax id: 871665
  • Matching level: species

strain history

@refhistory
21179<- KCTC; KCTC 5980
67770J.-W. Bae M25.
67771<- Jin-Woo Bae, Kyung Hee Univ.

doi: 10.13145/bacdive24769.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Blautia
  • species: Blautia faecis
  • full scientific name: Blautia faecis Park et al. 2013

@ref: 21179

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridia, not assigned to order

family: Lachnospiraceae

genus: Blautia

species: Blautia faecis

full scientific name: Blautia faecis Park et al. 2013

strain designation: M25

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
30457positive1.65 µm0.65 µmovoid-shapedno
67771no
67771positive

pigmentation

  • @ref: 30457
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 21179
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
21179positivegrowth37mesophilic
30457positiveoptimum37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

culture pH

  • @ref: 30457
  • ability: positive
  • type: optimum
  • pH: 6.2

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30457anaerobe
67771anaerobe

spore formation

@refspore formation
30457no
67771no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
30457581435-dehydro-D-gluconate+carbon source
3045722599arabinose+carbon source
3045733942ribose+carbon source
304574853esculin+hydrolysis
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
30457acid phosphatase+3.1.3.2
30457alpha-galactosidase+3.2.1.22
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
21179--++-+++/----------+-----------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
21179human faeces, healthy Korean maleRepublic of KoreaKORAsia
67770Human feces
67771From human faces, healthy Korean male(28 years old)Republic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Host#Human#Male
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_8701.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_4053;97_4974;98_6355;99_8701&stattab=map
  • Last taxonomy: Blautia
  • 16S sequence: NR_109014
  • Sequence Identity:
  • Total samples: 5086
  • aquatic counts: 40
  • animal counts: 5046

Safety information

risk assessment

  • @ref: 21179
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
21179Blautia faecis strain M25 16S ribosomal RNA gene, partial sequenceHM6261781262ena871665
67771Blautia faecis strain M25 16S ribosomal RNA, partial sequenceNR_1090141262nuccore871665

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Blautia faecis strain DSM 27629871665.33wgspatric871665
66792Blautia faecis DSM 276292901179768draftimg871665

GC content

@refGC-contentmethod
2117941.6
6777141.6thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno90.417yes
flagellatedno92.91no
gram-positiveyes92.873yes
anaerobicyes99.365no
halophileno84.548no
spore-formingno52.011no
thermophileno98.289yes
glucose-utilyes89.266no
aerobicno97.777no
glucose-fermentyes79.343no

External links

@ref: 21179

culture collection no.: DSM 27629, JCM 17205, KCTC 5980

straininfo link

  • @ref: 88595
  • straininfo: 400950

literature

  • topic: Phylogeny
  • Pubmed-ID: 22544782
  • title: Blautia faecis sp. nov., isolated from human faeces.
  • authors: Park SK, Kim MS, Bae JW
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.036541-0
  • year: 2012
  • mesh: Adult, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids, Feces/*microbiology, Fermentation, Gram-Positive Bacteria/*classification/genetics/isolation & purification, Humans, Male, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21179Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-27629Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27629)
30457Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172679228776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88595Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400950.1