Strain identifier

BacDive ID: 24749

Type strain: Yes

Species: Eilatimonas milleporae

Strain Designation: MD2

Strain history: DSM 25217 <-- N. Paramasivam and E. Ben-Dov; Ben-Gurion Univ. of the Negev, Israel; MD2.

NCBI tax ID(s): 911205 (species)

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General

@ref: 21249

BacDive-ID: 24749

DSM-Number: 25217

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, animal pathogen

description: Eilatimonas milleporae MD2 is an aerobe, mesophilic, Gram-negative animal pathogen that was isolated from lesion tissue of the hydrocoral Millepora dichotoma.

NCBI tax id

  • NCBI tax id: 911205
  • Matching level: species

strain history

@refhistory
21249<- E. Ben Dov, Ben-Gurion-Univ., Beer-Sheva, Israel; MD2 <- N. Paramasivam
67770DSM 25217 <-- N. Paramasivam and E. Ben-Dov; Ben-Gurion Univ. of the Negev, Israel; MD2.

doi: 10.13145/bacdive24749.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Kordiimonadales
  • family: Temperatibacteraceae
  • genus: Eilatimonas
  • species: Eilatimonas milleporae
  • full scientific name: Eilatimonas milleporae Paramasivam et al. 2013

@ref: 21249

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Temperatibacteraceae

genus: Eilatimonas

species: Eilatimonas milleporae

full scientific name: Eilatimonas milleporae Paramasivam et al. 2013

strain designation: MD2

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
30770negativerod-shapedyes
69480yes92.822
69480negative99.985

colony morphology

  • @ref: 21249
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 21249
  • name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/514
  • composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21249positivegrowth28mesophilic
30770positivegrowth22-37
30770positiveoptimum30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
30770positivegrowth6.2-8
30770positiveoptimum7.4

Physiology and metabolism

oxygen tolerance

  • @ref: 30770
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
30770no
69481no100
69480no99.997

halophily

@refsaltgrowthtested relationconcentration
30770NaClpositivegrowth1.5-6 %
30770NaClpositiveoptimum2 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3077017234glucose+carbon source
3077017716lactose+carbon source
3077017632nitrate+reduction

enzymes

@refvalueactivityec
30770catalase+1.11.1.6
30770gelatinase+
30770cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
21249lesion tissue of the hydrocoral Millepora dichotomaMillepora dichotomaGulf of Eilat (= Gulf of Aqaba), northern Red SeaIsraelISRAsia
67770Damaged tissue of a specimen of the hydrocoral Millepora dichotoma from the Gulf of EilatMillepora dichotomaRed SeaIsraelISRAsia

isolation source categories

Cat1Cat2Cat3
#Host#Invertebrates (Other)#Cnidaria (Corals)
#Host Body-Site#Other#Wound

taxonmaps

  • @ref: 69479
  • File name: preview.99_94332.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8946;96_38761;97_49348;98_64837;99_94332&stattab=map
  • Last taxonomy: Eilatimonas milleporae subclade
  • 16S sequence: LC127221
  • Sequence Identity:
  • Total samples: 359
  • soil counts: 5
  • aquatic counts: 334
  • animal counts: 19
  • plant counts: 1

Safety information

risk assessment

  • @ref: 21249
  • pathogenicity animal: yes
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
21249Eilatimonas milleporae strain MD2 16S ribosomal RNA gene, partial sequenceHQ2887811494ena911205
67770Eilatimonas milleporae gene for 16S ribosomal RNA, partial sequence, strain: JCM 30196LC1272211436ena911205

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Eilatimonas milleporae strain DSM 25217911205.3wgspatric911205
66792Eilatimonas milleporae DSM 252172728369464draftimg911205
67770Eilatimonas milleporae DSM 25217GCA_003688555contigncbi911205

GC content

@refGC-contentmethod
2124960.3high performance liquid chromatography (HPLC)
3077060.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes90.198yes
flagellatedno53.246no
gram-positiveno97.704yes
anaerobicno98.045yes
halophileyes54.855no
spore-formingno92.342no
thermophileno98.075no
glucose-utilyes83.434yes
aerobicyes85.104yes
glucose-fermentno91.874no

External links

@ref: 21249

culture collection no.: DSM 25217, JCM 30196, LMG 26586

straininfo link

  • @ref: 88577
  • straininfo: 400346

literature

  • topic: Phylogeny
  • Pubmed-ID: 23002050
  • title: Eilatimonas milleporae gen. nov., sp. nov., a marine bacterium isolated from the hydrocoral Millepora dichotoma.
  • authors: Paramasivam N, Ben-Dov E, Arotsker L, Kushmaro A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.043976-0
  • year: 2012
  • mesh: Alphaproteobacteria/*classification/genetics/isolation & purification, Animals, Anthozoa/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Indian Ocean, Israel, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysis
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmedjournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21249Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-25217Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25217)
30770Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172710128776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa102533211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
88577Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400346.1