Strain identifier
BacDive ID: 24656
Type strain: 
Species: Thermotoga caldifontis
Strain Designation: AZM44c09
Strain history: <- K. Mori, NBRC, NITE (Natl. Inst. of Technol. and Evaluation), Japan; AZM44c09 <- K. Mori {2009}
NCBI tax ID(s): 1508419 (species)
version 8.1 (current version)
General
@ref: 21065
BacDive-ID: 24656
DSM-Number: 23272
keywords: genome sequence, Bacteria, anaerobe, thermophilic
description: Thermotoga caldifontis AZM44c09 is an anaerobe, thermophilic bacterium that was isolated from water from a terrestrial hot spring collected from a crack in a concrete wall.
NCBI tax id
- NCBI tax id: 1508419
- Matching level: species
strain history
- @ref: 21065
- history: <- K. Mori, NBRC, NITE (Natl. Inst. of Technol. and Evaluation), Japan; AZM44c09 <- K. Mori {2009}
doi: 10.13145/bacdive24656.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/thermotogota
- domain: Bacteria
- phylum: Thermotogota
- class: Thermotogae
- order: Thermotogales
- family: Thermotogaceae
- genus: Thermotoga
- species: Thermotoga caldifontis
- full scientific name: Thermotoga caldifontis Mori et al. 2014
synonyms
- @ref: 20215
- synonym: Pseudothermotoga caldifontis
@ref: 21065
domain: Bacteria
phylum: Thermotogae
class: Thermotogae
order: Thermotogales
family: Thermotogaceae
genus: Thermotoga
species: Thermotoga caldifontis
full scientific name: Thermotoga caldifontis Mori et al. 2014
strain designation: AZM44c09
type strain: yes
Culture and growth conditions
culture medium
- @ref: 21065
- name: THERMOTOGA PROFUNDA MEDIUM (DSMZ Medium 1539)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1539
- composition: Name: THERMOTOGA PROFUNDA MEDIUM (DSMZ Medium 1539) Composition: Na2CO3 1.97824 g/l D-Glucose 1.97824 g/l Yeast extract 0.98912 g/l Na2S x 9 H2O 0.296736 g/l L-Cysteine HCl x H2O 0.296736 g/l NH4Cl 0.24728 g/l MgSO4 x 7 H2O 0.24728 g/l Nitrilotriacetic acid 0.126607 g/l KH2PO4 0.0890208 g/l K2HPO4 0.049456 g/l FeCl2 x 4 H2O 0.0098912 g/l NaCl 0.0098912 g/l CaCl2 x 2 H2O 0.00098912 g/l MnCl2 x 4 H2O 0.00098912 g/l ZnCl2 0.00098912 g/l NiCl2 x 6 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l Na2WO4 x 2 H2O 0.000395648 g/l Na2SeO3 x 5 H2O 0.000296736 g/l CoCl2 x 6 H2O 0.000296736 g/l Na2MoO4 x 2 H2O 0.000296736 g/l CuCl2 0.000197824 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Biotin 1.97824e-05 g/l Folic acid 1.97824e-05 g/l Vitamin B12 9.8912e-07 g/l Distilled water
culture temp
- @ref: 21065
- growth: positive
- type: growth
- temperature: 70
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 21065
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 21065
- sample type: water from a terrestrial hot spring collected from a crack in a concrete wall
- geographic location: Nagano Prefecture, Nakabusa hot spring
- country: Japan
- origin.country: JPN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Environmental | #Terrestrial | |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 21065
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermotoga caldifontis AZM44c09 | GCA_000828655 | complete | ncbi | 1408159 |
| 66792 | Thermotoga caldifontis AZM44c09 | 1408159.3 | complete | patric | 1408159 |
| 66792 | Thermotoga caldifontis AZM44c09 | 2791355055 | complete | img | 1408159 |
GC content
- @ref: 21065
- GC-content: 51.3
- method: sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | yes | 83.849 | no |
| flagellated | no | 94.561 | no |
| gram-positive | no | 94.781 | no |
| anaerobic | yes | 99.185 | no |
| aerobic | no | 97.574 | no |
| halophile | no | 90.407 | no |
| spore-forming | no | 88.828 | no |
| glucose-util | yes | 88.902 | no |
| thermophile | yes | 100 | no |
| glucose-ferment | no | 54.01 | no |
External links
@ref: 21065
culture collection no.: DSM 23272, NBRC 106116
straininfo link
- @ref: 88496
- straininfo: 406199
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24676729 | Thermotoga profunda sp. nov. and Thermotoga caldifontis sp. nov., anaerobic thermophilic bacteria isolated from terrestrial hot springs. | Mori K, Yamazoe A, Hosoyama A, Ohji S, Fujita N, Ishibashi JI, Kimura H, Suzuki KI | Int J Syst Evol Microbiol | 10.1099/ijs.0.060137-0 | 2014 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gram-Negative Anaerobic Straight, Curved, and Helical Rods/*classification/genetics/isolation & purification, Hot Springs/*microbiology, Japan, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Genetics |
| Phylogeny | 29801938 | Towards a congruent reclassification and nomenclature of the thermophilic species of the genus Pseudothermotoga within the order Thermotogales. | Belahbib H, Summers ZM, Fardeau ML, Joseph M, Tamburini C, Dolla A, Ollivier B, Armougom F | Syst Appl Microbiol | 10.1016/j.syapm.2018.04.007 | 2018 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Gram-Negative Anaerobic Straight, Curved, and Helical Rods/*classification/genetics, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Temperature | Transcriptome |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21065 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23272 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23272) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 88496 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID406199.1 |