Strain identifier

BacDive ID: 24654

Type strain: Yes

Species: Actinomadura xylanilytica

Strain Designation: BK147

Strain history: KACC 20919 <-- B.-Y. Kim; Univ. of Newcastle, UK; BK147.

NCBI tax ID(s): 887459 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21146

BacDive-ID: 24654

DSM-Number: 46742

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Actinomadura xylanilytica BK147 is an aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from hay meadow plot 6 soil.

NCBI tax id

  • NCBI tax id: 887459
  • Matching level: species

strain history

@refhistory
21146<- NRRL; NRRL B-24852
67770KACC 20919 <-- B.-Y. Kim; Univ. of Newcastle, UK; BK147.

doi: 10.13145/bacdive24654.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinomadura
  • species: Actinomadura xylanilytica
  • full scientific name: Actinomadura xylanilytica Zucchi et al. 2013

@ref: 21146

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Thermomonosporaceae

genus: Actinomadura

species: Actinomadura xylanilytica

full scientific name: Actinomadura xylanilytica Zucchi et al. 2013

strain designation: BK147

type strain: yes

Morphology

cell morphology

  • @ref: 30698
  • gram stain: positive
  • motility: no

colony morphology

@refcolony colormedium used
69470ColourlessISP 4
69470Grey white (9002)ISP 5
69470Light ivory (1015)ISP 3
69470Light ivory (1015)ISP 6
69470Light ivory (1015)ISP 7
69470Light ivory (1015), khaki grey (7008)suter with tyrosine
69470Oyster white (1013), khaki grey (7008)suter without tyrosine
69470Silk grey (7044)ISP 2

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69470yesAerial myceliumSignal white (9003)ISP 2
69470yesAerial myceliumTraffic white (9016)ISP 3
69470noAerial myceliumISP 4
69470yesAerial myceliumSignal white (9003)ISP 5
69470noAerial myceliumISP 6
69470yesAerial myceliumSignal white (9003)ISP 7
69470noAerial myceliumsuter with tyrosine
69470noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
30698yes
69470noMelanin
69470yessoluble pigmentIvory (1014), green beige (1000)

multimedia

  • @ref: 21146
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_46742.jpg
  • caption: Medium 553 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21146GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
21146GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21146positivegrowth28mesophilic
30698positivegrowth10-37
30698positiveoptimum23.5psychrophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
30698positivegrowth05-09alkaliphile
30698positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30698
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30698
  • spore formation: no

halophily

  • @ref: 69470
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-2.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H8), MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3069817306maltose+carbon source
306984853esculin+hydrolysis
6947022599arabinose-growth
6947062968cellulose+/-growth
6947028757fructose+growth
6947017234glucose+growth
6947017268inositol+/-growth
6947037684mannose+growth
6947016634raffinose-growth
6947026546rhamnose+/-growth
6947017992sucrose+/-growth
6947018222xylose-growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
30698gelatinase+
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69470---+------+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69470++/-+/--+++/---++-+/--+/-+---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
21146hay meadow plot 6 soilNorthumberland, Cockle Park Experimental FarmUnited KingdomGBREurope
67770Hay meadow plot 6 soil at Cockle Park Experimental FarmNorthumberlandUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Meadow
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 21146
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21146
  • description: Actinomadura xylanilytica partial 16S rRNA gene, type strain BK147T
  • accession: FR692101
  • length: 1454
  • database: ena
  • NCBI tax ID: 887459

External links

@ref: 21146

culture collection no.: DSM 46742, KACC 20919, NCIMB 14771, NRRL B-24852, JCM 32746, NBRC 109779

straininfo link

  • @ref: 88494
  • straininfo: 402369

literature

  • topic: Phylogeny
  • Pubmed-ID: 22523172
  • title: Actinomadura xylanilytica sp. nov., an actinomycete isolated from soil.
  • authors: Zucchi TD, Kim BY, Bonda ANV, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.042325-0
  • year: 2012
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Carbohydrates/analysis, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/analysis
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21146Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-46742Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46742)
30698Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172702928776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69470Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2046742.pdf
88494Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402369.1