Strain identifier
BacDive ID: 24654
Type strain:
Species: Actinomadura xylanilytica
Strain Designation: BK147
Strain history: KACC 20919 <-- B.-Y. Kim; Univ. of Newcastle, UK; BK147.
NCBI tax ID(s): 887459 (species)
General
@ref: 21146
BacDive-ID: 24654
DSM-Number: 46742
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive
description: Actinomadura xylanilytica BK147 is an aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from hay meadow plot 6 soil.
NCBI tax id
- NCBI tax id: 887459
- Matching level: species
strain history
@ref | history |
---|---|
21146 | <- NRRL; NRRL B-24852 |
67770 | KACC 20919 <-- B.-Y. Kim; Univ. of Newcastle, UK; BK147. |
doi: 10.13145/bacdive24654.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Streptosporangiales
- family: Thermomonosporaceae
- genus: Actinomadura
- species: Actinomadura xylanilytica
- full scientific name: Actinomadura xylanilytica Zucchi et al. 2013
@ref: 21146
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Thermomonosporaceae
genus: Actinomadura
species: Actinomadura xylanilytica
full scientific name: Actinomadura xylanilytica Zucchi et al. 2013
strain designation: BK147
type strain: yes
Morphology
cell morphology
- @ref: 30698
- gram stain: positive
- motility: no
colony morphology
@ref | colony color | medium used |
---|---|---|
69470 | Colourless | ISP 4 |
69470 | Grey white (9002) | ISP 5 |
69470 | Light ivory (1015) | ISP 3 |
69470 | Light ivory (1015) | ISP 6 |
69470 | Light ivory (1015) | ISP 7 |
69470 | Light ivory (1015), khaki grey (7008) | suter with tyrosine |
69470 | Oyster white (1013), khaki grey (7008) | suter without tyrosine |
69470 | Silk grey (7044) | ISP 2 |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
69470 | yes | Aerial mycelium | Signal white (9003) | ISP 2 |
69470 | yes | Aerial mycelium | Traffic white (9016) | ISP 3 |
69470 | no | Aerial mycelium | ISP 4 | |
69470 | yes | Aerial mycelium | Signal white (9003) | ISP 5 |
69470 | no | Aerial mycelium | ISP 6 | |
69470 | yes | Aerial mycelium | Signal white (9003) | ISP 7 |
69470 | no | Aerial mycelium | suter with tyrosine | |
69470 | no | Aerial mycelium | suter without tyrosine |
pigmentation
@ref | production | name | color |
---|---|---|---|
30698 | yes | ||
69470 | no | Melanin | |
69470 | yes | soluble pigment | Ivory (1014), green beige (1000) |
multimedia
- @ref: 21146
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_46742.jpg
- caption: Medium 553 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
21146 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf | |
21146 | GPHF-MEDIUM (DSMZ Medium 553) | yes | https://mediadive.dsmz.de/medium/553 | Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
21146 | positive | growth | 28 | mesophilic |
30698 | positive | growth | 10-37 | |
30698 | positive | optimum | 23.5 | psychrophilic |
67770 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
30698 | positive | growth | 05-09 | alkaliphile |
30698 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 30698
- oxygen tolerance: aerobe
spore formation
- @ref: 30698
- spore formation: no
halophily
- @ref: 69470
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-2.5 %
observation
- @ref: 67770
- observation: quinones: MK-9(H6), MK-9(H8), MK-9(H4)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30698 | 17306 | maltose | + | carbon source |
30698 | 4853 | esculin | + | hydrolysis |
69470 | 22599 | arabinose | - | growth |
69470 | 62968 | cellulose | +/- | growth |
69470 | 28757 | fructose | + | growth |
69470 | 17234 | glucose | + | growth |
69470 | 17268 | inositol | +/- | growth |
69470 | 37684 | mannose | + | growth |
69470 | 16634 | raffinose | - | growth |
69470 | 26546 | rhamnose | +/- | growth |
69470 | 17992 | sucrose | +/- | growth |
69470 | 18222 | xylose | - | growth |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
30698 | gelatinase | + | |
68379 | gelatinase | + | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | - | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69470 | - | - | - | + | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69470 | + | +/- | +/- | - | + | + | +/- | - | - | + | + | - | +/- | - | +/- | + | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
21146 | hay meadow plot 6 soil | Northumberland, Cockle Park Experimental Farm | United Kingdom | GBR | Europe |
67770 | Hay meadow plot 6 soil at Cockle Park Experimental Farm | Northumberland | United Kingdom | GBR | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Agriculture | #Meadow |
#Environmental | #Terrestrial | #Soil |
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
Safety information
risk assessment
- @ref: 21146
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21146
- description: Actinomadura xylanilytica partial 16S rRNA gene, type strain BK147T
- accession: FR692101
- length: 1454
- database: ena
- NCBI tax ID: 887459
External links
@ref: 21146
culture collection no.: DSM 46742, KACC 20919, NCIMB 14771, NRRL B-24852, JCM 32746, NBRC 109779
straininfo link
- @ref: 88494
- straininfo: 402369
literature
- topic: Phylogeny
- Pubmed-ID: 22523172
- title: Actinomadura xylanilytica sp. nov., an actinomycete isolated from soil.
- authors: Zucchi TD, Kim BY, Bonda ANV, Goodfellow M
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.042325-0
- year: 2012
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Carbohydrates/analysis, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/analysis
- topic2: Genetics
Reference
@id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
21146 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-46742 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46742) | |||
30698 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27029 | 28776041 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69470 | Wink, J. | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | https://cdn.dsmz.de/wink/DSM%2046742.pdf | |||
88494 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402369.1 |