Strain identifier
BacDive ID: 24650
Type strain: 
Species: Caldanaerobius polysaccharolyticus
Strain Designation: KMTHCJ
Strain history: <- P. G. Stroot; KMTHCJ <- I. O. K. Cann and P. G. Stroot
NCBI tax ID(s): 935948 (strain), 44256 (species)
version 8.1 (current version)
General
@ref: 21060
BacDive-ID: 24650
DSM-Number: 13641
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive
description: Caldanaerobius polysaccharolyticus KMTHCJ is an anaerobe, spore-forming, thermophilic bacterium that was isolated from organic waste leachate.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 44256 | species |
| 935948 | strain |
strain history
- @ref: 21060
- history: <- P. G. Stroot; KMTHCJ <- I. O. K. Cann and P. G. Stroot
doi: 10.13145/bacdive24650.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Caldanaerobius
- species: Caldanaerobius polysaccharolyticus
- full scientific name: Caldanaerobius polysaccharolyticus (Cann et al. 2001) Lee et al. 2008
synonyms
- @ref: 20215
- synonym: Thermoanaerobacterium polysaccharolyticum
@ref: 21060
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Caldanaerobius
species: Caldanaerobius polysaccharolyticus
full scientific name: Caldanaerobius polysaccharolyticus (Cann et al. 2001) Lee et al. 2008
strain designation: KMTHCJ
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 99.719
multimedia
| @ref | multimedia content | caption | intellectual property rights |
|---|---|---|---|
| 66793 | EM_DSM_13641_1.jpg | electron microscopic image | © HZI/Manfred Rohde |
| 66793 | EM_DSM_13641_2.jpg | electron microscopic image | © HZI/Manfred Rohde |
| 66793 | EM_DSM_13641_3.jpg | electron microscopic image | © HZI/Manfred Rohde |
| 66793 | EM_DSM_13641_4.jpg | electron microscopic image | © HZI/Manfred Rohde |
| 66793 | EM_DSM_13641_5.jpg | electron microscopic image | © HZI/Manfred Rohde |
Culture and growth conditions
culture medium
- @ref: 21060
- name: CLOSTRIDIUM LJUNGDAHLII MEDIUM (DSMZ Medium 879)
- growth: yes
- link: https://mediadive.dsmz.de/medium/879
- composition: Name: CLOSTRIDIUM LJUNGDAHLII MEDIUM (DSMZ Medium 879; with strain-specific modifications) Composition: Sucrose 4.9456 g/l NaHCO3 2.4728 g/l NH4Cl 0.98912 g/l Yeast extract 0.98912 g/l Na2S x 9 H2O 0.296736 g/l L-Cysteine HCl x H2O 0.296736 g/l KCl 0.098912 g/l KH2PO4 0.098912 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l ZnSO4 x 7 H2O 0.00178042 g/l CoSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 21060 | positive | growth | 65 | thermophilic | |
| 69480 | thermophilic | 100 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 21060 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
- @ref: 69480
- spore formation: yes
- confidence: 94.429
Isolation, sampling and environmental information
isolation
- @ref: 21060
- sample type: organic waste leachate
- geographic location: Illinois, Hoopeston
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
- Cat1: #Engineered
- Cat2: #Waste
- Cat3: #Wastewater
taxonmaps
- @ref: 69479
- File name: preview.99_45586.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_679;96_3675;97_4514;98_5798;99_45586&stattab=map
- Last taxonomy: Caldanaerobius polysaccharolyticus subclade
- 16S sequence: U40229
- Sequence Identity:
- Total samples: 7
- soil counts: 1
- aquatic counts: 5
- animal counts: 1
Safety information
risk assessment
- @ref: 21060
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21060
- description: Thermoanaerobacterium polysaccharolyticum 16S ribosomal RNA gene, complete sequence
- accession: U40229
- length: 1438
- database: ena
- NCBI tax ID: 935948
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Caldanaerobius polysaccharolyticus DSM 13641 | GCA_000427425 | scaffold | ncbi | 935948 |
| 66792 | Caldanaerobius polysaccharolyticus DSM 13641 | 935948.5 | wgs | patric | 935948 |
| 66792 | Caldanaerobius polysaccharolyticus KMTHCJ, DSM 13641 | 2510065085 | draft | img | 935948 |
GC content
- @ref: 21060
- GC-content: 46
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 88 | no |
| motile | yes | 79.22 | no |
| flagellated | no | 85.344 | no |
| gram-positive | yes | 61.095 | no |
| anaerobic | yes | 98.412 | yes |
| aerobic | no | 96.61 | yes |
| halophile | no | 96.077 | no |
| spore-forming | yes | 90.481 | no |
| glucose-util | yes | 90.575 | no |
| thermophile | yes | 100 | yes |
| glucose-ferment | yes | 56.339 | no |
External links
@ref: 21060
culture collection no.: DSM 13641
straininfo link
- @ref: 88490
- straininfo: 49499
literature
- topic: Phylogeny
- Pubmed-ID: 11321073
- title: Characterization of two novel saccharolytic, anaerobic thermophiles, Thermoanaerobacterium polysaccharolyticum sp. nov. and Thermoanaerobacterium zeae sp. nov., and emendation of the genus Thermoanaerobacterium.
- authors: Cann IK, Stroot PG, Mackie KR, White BA, Mackie RI
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/00207713-51-2-293
- year: 2001
- mesh: Bacteria, Anaerobic/*classification/metabolism/ultrastructure, Bacterial Typing Techniques, Base Composition, DNA, Ribosomal/genetics, Environmental Microbiology, Food-Processing Industry, Gram-Positive Asporogenous Rods/*classification/metabolism/ultrastructure, *Hot Temperature, Industrial Waste, Molecular Sequence Data, Polysaccharides/*metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Terminology as Topic, Thiosulfates/metabolism
- topic2: Metabolism
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 21060 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13641 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13641) | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 66793 | Mukherjee et al. | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 10.1038/nbt.3886 | 35: 676-683 2017 | 28604660 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 88490 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID49499.1 |