Strain identifier
BacDive ID: 24646
Type strain: 
Species: Lihuaxuella thermophila
Strain history: S.-K. Tang YIM 77831.
NCBI tax ID(s): 1173111 (species)
version 8.1 (current version)
General
@ref: 21243
BacDive-ID: 24646
DSM-Number: 46701
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, thermophilic, Gram-positive
description: Lihuaxuella thermophila DSM 46701 is a spore-forming, thermophilic, Gram-positive bacterium that was isolated from geothermal soil.
NCBI tax id
- NCBI tax id: 1173111
- Matching level: species
strain history
| @ref | history |
|---|---|
| 21243 | <- JCM; JCM 18059 <- S.-K. Tang; YIM 77831 |
| 67770 | S.-K. Tang YIM 77831. |
doi: 10.13145/bacdive24646.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Thermoactinomycetaceae
- genus: Lihuaxuella
- species: Lihuaxuella thermophila
- full scientific name: Lihuaxuella thermophila Yu et al. 2013
@ref: 21243
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Thermoactinomycetaceae
genus: Lihuaxuella
species: Lihuaxuella thermophila
full scientific name: Lihuaxuella thermophila Yu et al. 2013
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 21243 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
| 21243 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 21243 | positive | growth | 50 | thermophilic |
| 67770 | positive | growth | 50 | thermophilic |
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
observation
- @ref: 67770
- observation: quinones: MK-7
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 21243 | geothermal soil | Yunnan province, Tengchong, Rehai National Park | China | CHN | Asia |
| 67770 | Geothermal soil from the south-west China |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Soil |
| #Environmental | #Terrestrial | #Geologic |
taxonmaps
- @ref: 69479
- File name: preview.99_76736.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_284;96_32494;97_41147;98_53643;99_76736&stattab=map
- Last taxonomy: Lihuaxuella thermophila subclade
- 16S sequence: JQ750619
- Sequence Identity:
- Total samples: 603
- soil counts: 259
- aquatic counts: 60
- animal counts: 251
- plant counts: 33
Safety information
risk assessment
- @ref: 21243
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 21243 | Lihuaxuella thermophila strain YIM 77831 16S ribosomal RNA gene, partial sequence | JX045707 | 1516 | ena | 1173111 |
| 67770 | Lihuaxuella thermophila strain YIM 77831 16S ribosomal RNA gene, partial sequence | JQ750619 | 1497 | ena | 1173111 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Lihuaxuella thermophila strain DSM 46701 | 1173111.3 | wgs | patric | 1173111 |
| 66792 | Lihuaxuella thermophila DSM 46701 | 2675903160 | draft | img | 1173111 |
| 67770 | Lihuaxuella thermophila DSM 46701 | GCA_900110165 | scaffold | ncbi | 1173111 |
GC content
- @ref: 21243
- GC-content: 55.6
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | no | 83.323 | no |
| gram-positive | yes | 87.617 | no |
| anaerobic | no | 98.09 | no |
| halophile | no | 93.286 | no |
| spore-forming | yes | 95.548 | no |
| flagellated | no | 86.56 | no |
| thermophile | yes | 99.361 | yes |
| glucose-util | yes | 90.998 | no |
| aerobic | yes | 94.243 | no |
| glucose-ferment | no | 94.112 | no |
External links
@ref: 21243
culture collection no.: DSM 46701, CCTCC AA 2011024, JCM 18059, YIM 77831
straininfo link
- @ref: 88486
- straininfo: 397960
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 22760842 | Lihuaxuella thermophila gen. nov., sp. nov., isolated from a geothermal soil sample in Tengchong, Yunnan, south-west China. | Yu TT, Zhang BH, Yao JC, Tang SK, Zhou EM, Yin YR, Wei DQ, Ming H, Li WJ | Antonie Van Leeuwenhoek | 10.1007/s10482-012-9771-6 | 2012 | Bacillales/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Carbohydrates/analysis, Cell Wall/chemistry, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial/cytology, Temperature, Vitamin K 2/analysis | Genetics |
| Phylogeny | 25991661 | Thermoactinomyces guangxiensis sp. nov., a thermophilic actinomycete isolated from mushroom compost. | Wu H, Liu B, Pan S | Int J Syst Evol Microbiol | 10.1099/ijs.0.000342 | 2015 | *Agaricales, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, *Soil Microbiology, Spores, Bacterial, Thermoactinomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives | Genetics |
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 21243 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-46701 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46701) | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
| 88486 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID397960.1 |