Strain identifier
BacDive ID: 24645
Type strain: 
Species: Melghirimyces thermohalophilus
Strain Designation: 11A, Nari11A
Strain history: <- M.-L. Fardeau, IRD, Marseille, France; 11A <- N. A. Addou
NCBI tax ID(s): 1236220 (species)
version 8.1 (current version)
General
@ref: 21235
BacDive-ID: 24645
DSM-Number: 45514
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive
description: Melghirimyces thermohalophilus 11A is an aerobe, spore-forming, thermophilic bacterium that was isolated from soil from a salt lake.
NCBI tax id
- NCBI tax id: 1236220
- Matching level: species
strain history
- @ref: 21235
- history: <- M.-L. Fardeau, IRD, Marseille, France; 11A <- N. A. Addou
doi: 10.13145/bacdive24645.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Thermoactinomycetaceae
- genus: Melghirimyces
- species: Melghirimyces thermohalophilus
- full scientific name: Melghirimyces thermohalophilus Addou et al. 2013
@ref: 21235
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Thermoactinomycetaceae
genus: Melghirimyces
species: Melghirimyces thermohalophilus
full scientific name: Melghirimyces thermohalophilus Addou et al. 2013
strain designation: 11A, Nari11A
type strain: yes
Morphology
cell morphology
| @ref | gram stain | confidence |
|---|---|---|
| 30760 | positive | |
| 69480 | positive | 100 |
colony morphology
- @ref: 62194
- incubation period: 3 days
pigmentation
- @ref: 30760
- production: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 21235 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
| 21235 | GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) | yes | https://mediadive.dsmz.de/medium/1159 | Name: GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) Composition: NaCl 100.0 g/l Agar 15.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 30760 | positive | growth | 43-60 | thermophilic |
| 62194 | positive | growth | 56 | thermophilic |
| 21235 | positive | growth | 55 | thermophilic |
culture pH
- @ref: 30760
- ability: positive
- type: growth
- pH: 05-10
- PH range: alkaliphile
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 30760 | aerobe |
| 62194 | aerobe |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 30760 | yes | |
| 69481 | yes | 100 |
| 69480 | yes | 98.149 |
halophily
- @ref: 30760
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 05-20 %
metabolite utilization
- @ref: 30760
- Chebi-ID: 4853
- metabolite: esculin
- utilization activity: +
- kind of utilization tested: hydrolysis
enzymes
- @ref: 30760
- value: gelatinase
- activity: +
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | sampling date |
|---|---|---|---|---|---|---|
| 21235 | soil from a salt lake | Chott Melghir (located between 34° 00' 00'' N 6° 07' 30'' E and 34°30' 01'' N 6° 30' 02'' E) | Algeria | DZA | Africa | |
| 62194 | Soil | Chott Melghir salt lake,Baskra City | Algeria | DZA | Africa | 2006 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Terrestrial | #Soil |
| #Condition | #Saline |
Safety information
risk assessment
- @ref: 21235
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21235
- description: Melghirimyces thermohalophilus strain Nari11A 16S ribosomal RNA gene, partial sequence
- accession: JX861508
- length: 1541
- database: ena
- NCBI tax ID: 1236220
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Melghirimyces thermohalophilus DSM 45514 | GCA_900102685 | contig | ncbi | 1236220 |
| 66792 | Melghirimyces thermohalophilus strain DSM 45514 | 1236220.3 | wgs | patric | 1236220 |
| 66792 | Melghirimyces thermohalophilus DSM 45514 | 2615840705 | draft | img | 1236220 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 21235 | 53.4 | high performance liquid chromatography (HPLC) |
| 30760 | 53.4 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | no | 79.487 | no |
| gram-positive | yes | 92.03 | yes |
| anaerobic | no | 98.641 | yes |
| aerobic | yes | 94.838 | yes |
| halophile | no | 56.988 | no |
| spore-forming | yes | 92.495 | yes |
| glucose-util | yes | 92.728 | no |
| flagellated | no | 93.887 | no |
| thermophile | yes | 99.395 | yes |
| glucose-ferment | no | 92.448 | no |
External links
@ref: 21235
culture collection no.: DSM 45514, CCUG 60050
straininfo link
- @ref: 88485
- straininfo: 400239
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 23041632 | Melghirimyces thermohalophilus sp. nov., a thermoactinomycete isolated from an Algerian salt lake. | Addou AN, Schumann P, Sproer C, Bouanane-Darenfed A, Amarouche-Yala S, Hacene H, Cayol JL, Fardeau ML | Int J Syst Evol Microbiol | 10.1099/ijs.0.043760-0 | 2012 | Algeria, Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Lakes/*microbiology, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil/analysis, Vitamin K 2/analysis, Water Microbiology | Genetics |
| Phylogeny | 23912721 | Melghirimyces profundicolus sp. nov., isolated from a deep-sea sediment. | Li J, Qin S, You ZQ, Long LJ, Tian XP, Wang FZ, Zhang S | Int J Syst Evol Microbiol | 10.1099/ijs.0.052175-0 | 2013 | Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Indian Ocean, Molecular Sequence Data, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Reference
| @id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||||
| 21235 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45514 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45514) | ||||
| 30760 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27091 | 28776041 | ||
| 62194 | Curators of the CCUG | https://www.ccug.se/strain?id=60050 | Culture Collection University of Gothenburg (CCUG) (CCUG 60050) | ||||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | ||||
| 88485 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID400239.1 |