Strain identifier

BacDive ID: 24488

Type strain: Yes

Species: Gemmobacter megaterium

Strain Designation: CF17

Strain history: X.-Q. Zhang CF17.

NCBI tax ID(s): 1086013 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 20578

BacDive-ID: 24488

DSM-Number: 26375

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative

description: Gemmobacter megaterium CF17 is a mesophilic, Gram-negative bacterium that was isolated from planktonic seaweed from coastal seawater.

NCBI tax id

  • NCBI tax id: 1086013
  • Matching level: species

strain history

@refhistory
20578<- J. Liu, Zhejiang Univ., College of Life Scs., Hangzhou, China; CF17 <- F.-T. Chi
67770X.-Q. Zhang CF17.

doi: 10.13145/bacdive24488.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Paracoccaceae
  • genus: Gemmobacter
  • species: Gemmobacter megaterium
  • full scientific name: Gemmobacter megaterium Liu et al. 2014
  • synonyms

    • @ref: 20215
    • synonym: Wagnerdoeblera megaterium

@ref: 20578

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Gemmobacter

species: Gemmobacter megaterium

full scientific name: Gemmobacter megaterium Liu et al. 2014

strain designation: CF17

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.993

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
20578BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
20578ONR7a MEDIUM (DSMZ Medium 950)yeshttps://mediadive.dsmz.de/medium/950Name: ONR7a MEDIUM (DSMZ Medium 950; with strain-specific modifications) Composition: NaCl 22.79 g/l Agar 15.0 g/l Agarose 12.0 g/l MgCl2 x 6 H2O 11.18 g/l Sodium pyruvate 10.0 g/l Na2SO4 3.98 g/l CaCl2 x 2 H2O 1.46 g/l TAPSO 1.3 g/l KCl 0.72 g/l NH4Cl 0.27 g/l Na2HPO4 x 7 H2O 0.089 g/l NaBr 0.083 g/l NaHCO3 0.031 g/l H3BO3 0.027 g/l SrCl2 x 6 H2O 0.024 g/l NaF 0.0026 g/l FeCl2 x 4 H2O 0.002 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
20578positivegrowth28mesophilic
67770positivegrowth27mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.996

observation

  • @ref: 67770
  • observation: quinones: Q-10

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
20578planktonic seaweed from coastal seawaterEast China Sea, Zhoushan sea areaChinaCHNAsia
67770Coastal planktonic seaweedsEast China Sea

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Coast
#Host#Algae

taxonmaps

  • @ref: 69479
  • File name: preview.99_5155.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_926;97_1078;98_3842;99_5155&stattab=map
  • Last taxonomy: Gemmobacter megaterium subclade
  • 16S sequence: JN620361
  • Sequence Identity:
  • Total samples: 110
  • soil counts: 6
  • aquatic counts: 85
  • animal counts: 17
  • plant counts: 2

Safety information

risk assessment

  • @ref: 20578
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20578
  • description: Gemmobacter megaterium strain CF17 16S ribosomal RNA gene, partial sequence
  • accession: JN620361
  • length: 1428
  • database: ena
  • NCBI tax ID: 1086013

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Gemmobacter megaterium CGMCC 1.11024GCA_014636395scaffoldncbi1086013
66792Gemmobacter megaterium strain CGMCC 1.110241086013.8wgspatric1086013
66792Gemmobacter megaterium strain DSM 263751086013.3wgspatric1086013
66792Gemmobacter megaterium DSM 263752681813556draftimg1086013
67770Gemmobacter megaterium DSM 26375GCA_900156815contigncbi1086013

GC content

  • @ref: 20578
  • GC-content: 61.4
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno69no
flagellatedno90.577no
gram-positiveno98.094no
anaerobicno98.444no
aerobicyes89.47no
halophileno56.505no
spore-formingno95.813no
glucose-utilyes51.661no
thermophileno97.507yes
motileno66.344no
glucose-fermentno89.77no

External links

@ref: 20578

culture collection no.: DSM 26375, CGMCC 1.11024, JCM 18498

straininfo link

  • @ref: 88348
  • straininfo: 407198

literature

  • topic: Phylogeny
  • Pubmed-ID: 24014623
  • title: Gemmobacter megaterium sp. nov., isolated from coastal planktonic seaweeds.
  • authors: Liu JJ, Zhang XQ, Chi FT, Pan J, Sun C, Wu M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.050955-0
  • year: 2013
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater, Seaweed/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20578Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26375Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26375)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
88348Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407198.1