Strain identifier

BacDive ID: 24481

Type strain: Yes

Species: Rhizobium mongolense subsp. loessense

Strain history: CIP <- 2003, W.X. Chen, China Agric. Univ., Beijing, China: strain CCBAU 7190B

NCBI tax ID(s): 158890 (subspecies)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21027

BacDive-ID: 24481

DSM-Number: 21811

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped

description: Rhizobium mongolense subsp. loessense DSM 21811 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from root nodule of Astragalus complanatus.

NCBI tax id

  • NCBI tax id: 158890
  • Matching level: subspecies

strain history

@refhistory
21027<- X. Sui, China Agric. Univ., College Biol. Sci., Beijing; CCBAU 7190B <- G. H. Wei et al.
366822003, W. X. Chen, China Agric. Univ., Beijing, China: strain CCBAU 7190B
120641CIP <- 2003, W.X. Chen, China Agric. Univ., Beijing, China: strain CCBAU 7190B

doi: 10.13145/bacdive24481.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Rhizobiaceae
  • genus: Rhizobium
  • species: Rhizobium mongolense subsp. loessense
  • full scientific name: Rhizobium mongolense subsp. loessense (Wei et al. 2003) Hördt et al. 2020
  • synonyms

    • @ref: 20215
    • synonym: Rhizobium loessense

@ref: 21027

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Hyphomicrobiales

family: Rhizobiaceae

genus: Rhizobium

species: Rhizobium mongolense subsp. loessense

full scientific name: Rhizobium mongolense subsp. loessense (Wei et al. 2003) Hördt et al. 2020

type strain: yes

Morphology

cell morphology

  • @ref: 120641
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21027YMA-MEDIUM (MODIFIED) (DSMZ Medium 1031a)yeshttps://mediadive.dsmz.de/medium/1031aName: YMA-MEDIUM (modified) (DSMZ Medium 1031a) Composition: Agar 20.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l K2HPO4 0.5 g/l Na glutamate 0.5 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.0528 g/l NaCl 0.05 g/l FeCl3 x 6 H2O 0.00666 g/l Distilled water
36682MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
120641CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
21027positivegrowth28mesophilic
36682positivegrowth30mesophilic
120641positivegrowth25-37mesophilic
120641nogrowth10psychrophilic
120641nogrowth41thermophilic
120641nogrowth45thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
120641NaClnogrowth0 %
120641NaClnogrowth2 %
120641NaClnogrowth4 %
120641NaClnogrowth6 %
120641NaClnogrowth8 %
120641NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120641citrate-carbon source16947
120641esculin+hydrolysis4853
120641nitrate-reduction17632
120641nitrite-reduction16301

metabolite production

  • @ref: 120641
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12064115688acetoin-
12064117234glucose-

enzymes

@refvalueactivityec
120641oxidase+
120641beta-galactosidase+3.2.1.23
120641alcohol dehydrogenase-1.1.1.1
120641gelatinase-
120641amylase-
120641DNase-
120641caseinase-3.4.21.50
120641catalase+1.11.1.6
120641tween esterase-
120641lecithinase-
120641lipase-
120641lysine decarboxylase-4.1.1.18
120641ornithine decarboxylase-4.1.1.17
120641phenylalanine ammonia-lyase-4.3.1.24
120641tryptophan deaminase-
120641urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120641-+++-+--+-+----+++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120641-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120641+-+++-+-++-+++++++++++----+++--++-+--+-++--+---------------++---+-----+----------------------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
21027root nodule of Astragalus complanatusAstragalus complanatusGan SuChinaCHNAsia
120641Astragalus complanatusGansuChinaCHNAsia2001

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host#Plants#Shrub (Scrub)
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_3467.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_62;97_2170;98_2662;99_3467&stattab=map
  • Last taxonomy: Rhizobium
  • 16S sequence: AF364069
  • Sequence Identity:
  • Total samples: 270
  • soil counts: 105
  • aquatic counts: 5
  • animal counts: 6
  • plant counts: 154

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
210271Risk group (German classification)
1206411Risk group (French classification)

Sequence information

16S sequences

  • @ref: 21027
  • description: Rhizobium loessense strain CCBAU 7190B 16S ribosomal RNA gene, partial sequence
  • accession: AF364069
  • length: 1379
  • database: ena
  • NCBI tax ID: 158890

GC content

  • @ref: 21027
  • GC-content: 59.5

External links

@ref: 21027

culture collection no.: DSM 21811, CCBAU 7190B, CIP 108030, LMG 21975, AS 1.3401

straininfo link

  • @ref: 88342
  • straininfo: 398753

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130051Characterization of rhizobia isolated from legume species within the genera Astragalus and Lespedeza grown in the Loess Plateau of China and description of Rhizobium loessense sp. nov.Wei GH, Tan ZY, Zhu ME, Wang ET, Han SZ, Chen WXInt J Syst Evol Microbiol10.1099/ijs.0.02031-02003Astragalus Plant/*microbiology, Base Composition, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Genes, Bacterial, Lespedeza/*microbiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Rhizobium/*classification/genetics/*isolation & purification/metabolismGenetics
Phylogeny23203621Rhizobium tarimense sp. nov., isolated from soil in the ancient Khiyik River.Turdahon M, Osman G, Hamdun M, Yusuf K, Abdurehim Z, Abaydulla G, Abdukerim M, Fang C, Rahman EInt J Syst Evol Microbiol10.1099/ijs.0.042176-02012Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizobium/*classification/genetics/isolation & purification, Rivers, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analogs & derivatives/analysisGenetics
Phylogeny24478208Rhizobium azibense sp. nov., a nitrogen fixing bacterium isolated from root-nodules of Phaseolus vulgaris.Mnasri B, Liu TY, Saidi S, Chen WF, Chen WX, Zhang XX, Mhamdi RInt J Syst Evol Microbiol10.1099/ijs.0.058651-02014Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Mexico, Molecular Sequence Data, *Nitrogen Fixation, Nucleic Acid Hybridization, Phaseolus/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizobium/*classification/genetics/isolation & purification/metabolism, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Spain, TunisiaGenetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21027Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-21811Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21811)
36682Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5608
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88342Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398753.1
120641Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108030Collection of Institut Pasteur (CIP 108030)