Strain identifier
BacDive ID: 24447
Type strain: 
Species: Pseudonocardia cypriaca
Strain Designation: KT2142, 5DSM 45511
Strain history: <- Nevzat Sahin, Ondokuz Mayis Univ., Turkey
NCBI tax ID(s): 882449 (species)
version 8.1 (current version)
General
@ref: 20749
BacDive-ID: 24447
DSM-Number: 45511
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive
description: Pseudonocardia cypriaca KT2142 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.
NCBI tax id
- NCBI tax id: 882449
- Matching level: species
strain history
| @ref | history |
|---|---|
| 20749 | <- N. Sahin, Ondokuz Mayis University; KT2142 |
| 67771 | <- Nevzat Sahin, Ondokuz Mayis Univ., Turkey |
doi: 10.13145/bacdive24447.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Pseudonocardia
- species: Pseudonocardia cypriaca
- full scientific name: Pseudonocardia cypriaca Sahin et al. 2014
@ref: 20749
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Pseudonocardiaceae
genus: Pseudonocardia
species: Pseudonocardia cypriaca
full scientific name: Pseudonocardia cypriaca Sahin et al. 2014
strain designation: KT2142, 5DSM 45511
type strain: yes
Morphology
cell morphology
| @ref | gram stain | confidence |
|---|---|---|
| 67771 | positive | |
| 69480 | positive | 100 |
Culture and growth conditions
culture medium
- @ref: 20749
- name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
- growth: yes
- link: https://mediadive.dsmz.de/medium/65
- composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 20749 | positive | growth | 28 | mesophilic |
| 67771 | positive | growth | 28 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 67771
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 94 |
| 69480 | yes | 99.727 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 20749 | soil | Magusa, Karpaz | Cyprus | CYP | Europe |
| 67771 | From soil, Agricultural estate | Cyprus | CYP | Europe |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
- @ref: 20749
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20749
- description: Pseudonocardia cypriaca strain KT2142 16S ribosomal RNA gene, partial sequence
- accession: HQ157191
- length: 1469
- database: ena
- NCBI tax ID: 882449
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Pseudonocardia cypriaca DSM 45511 | GCA_006717045 | contig | ncbi | 882449 |
| 66792 | Pseudonocardia cypriaca strain DSM 45511 | 882449.3 | wgs | patric | 882449 |
| 66792 | Pseudonocardia cypriaca DSM 45511 | 2818991232 | draft | img | 882449 |
GC content
- @ref: 20749
- GC-content: 74.6
- method: fluorimetric
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 94 | no |
| motile | no | 87.514 | no |
| flagellated | no | 97.793 | no |
| gram-positive | yes | 87.121 | yes |
| anaerobic | no | 99.131 | yes |
| halophile | no | 89.842 | no |
| spore-forming | yes | 84.244 | no |
| thermophile | no | 99.026 | no |
| glucose-util | yes | 82.081 | no |
| aerobic | yes | 91.091 | yes |
| glucose-ferment | no | 88.607 | no |
External links
@ref: 20749
culture collection no.: DSM 45511, KCTC 29067, NRRL B-24882
straininfo link
- @ref: 88324
- straininfo: 405988
literature
- topic: Phylogeny
- Pubmed-ID: 24523445
- title: Pseudonocardia cypriaca sp. nov., Pseudonocardia salamisensis sp. nov., Pseudonocardia hierapolitana sp. nov. and Pseudonocardia kujensis sp. nov., isolated from soil.
- authors: Sahin N, Veyisoglu A, Tatar D, Sproer C, Cetin D, Guven K, Klenk HP
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.059824-0
- year: 2014
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Cell Wall/chemistry, Cyprus, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nigeria, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/analysis/genetics, *Soil Microbiology, Turkey, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Genetics
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 20749 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45511 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45511) | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
| 88324 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID405988.1 |