Strain identifier
BacDive ID: 24436
Type strain:
Species: Pseudomonas entomophila
Strain Designation: L48
Strain history: <- CECT <- B. Lemaitre, Ecole Polytechnique Fédérale de Lausanne, Switzerland <- N. Vodovar
NCBI tax ID(s): 312306 (species)
General
@ref: 21139
BacDive-ID: 24436
DSM-Number: 28517
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, animal pathogen
description: Pseudomonas entomophila L48 is an aerobe, mesophilic animal pathogen that was isolated from Drosophila melanogaster.
NCBI tax id
- NCBI tax id: 312306
- Matching level: species
strain history
- @ref: 21139
- history: <- CECT <- B. Lemaitre, Ecole Polytechnique Fédérale de Lausanne, Switzerland <- N. Vodovar
doi: 10.13145/bacdive24436.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas entomophila
- full scientific name: Pseudomonas entomophila Mulet et al. 2012
@ref: 21139
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas entomophila
full scientific name: Pseudomonas entomophila Mulet et al. 2012
strain designation: L48
type strain: yes
Morphology
multimedia
- @ref: 21139
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_28517.jpg
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
- @ref: 21139
- name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1a
- composition: Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
21139 | positive | growth | 28 | mesophilic |
62634 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 62634
- oxygen tolerance: aerobe
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | + | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 62634 C12:0 1.5 12 62634 C15:0 0.5 15 62634 C16:0 25.1 16 62634 C10:0 3OH 9.4 11.423 62634 C12:0 2OH 6.8 13.178 62634 C12:0 3OH 5 13.455 62634 C16:1 ω7c 14.7 15.819 62634 C16:1 ω7c DMA 1.4 16.288 62634 C17:0 CYCLO 13.1 16.888 62634 C18:1 ω7c /12t/9t 12.2 17.824 62634 C18:1 ω9c 0.9 17.769 62634 C18:2 ω6,9c/C18:0 ANTE 1.3 17.724 62634 Unidentified 0.4 9.395 62634 Unidentified 1.1 9.983 62634 Unidentified 1.6 10.473 62634 Unidentified 4.4 10.651 62634 Unidentified 0.7 11.196 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
21139 | - | - | - | + | - | + | + | - | + | - | + | + | + | - | + | + | - | + | + | + | + |
21139 | - | - | - | + | - | - | - | - | + | - | + | + | + | - | + | + | - | + | + | + | + |
21139 | - | - | - | + | - | - | + | - | + | - | + | + | + | - | + | + | - | + | + | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent | sampling date |
---|---|---|---|---|---|---|---|
21139 | Drosophila melanogaster | Drosophila melanogaster | Calvaire (Guadeloupe) | France | FRA | Europe | |
62634 | Drosophila | Guadeloupe, Calvaire | France | FRA | Europe | 2000-02-01 |
isolation source categories
- Cat1: #Host
- Cat2: #Arthropoda
- Cat3: #Insecta
taxonmaps
- @ref: 69479
- File name: preview.99_239.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_203;99_239&stattab=map
- Last taxonomy: Pseudomonas
- 16S sequence: AY907566
- Sequence Identity:
- Total samples: 2133
- soil counts: 368
- aquatic counts: 543
- animal counts: 799
- plant counts: 423
Safety information
risk assessment
- @ref: 21139
- pathogenicity animal: yes
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21139
- description: Pseudomonas entomophila strain L48 16S ribosomal RNA gene, partial sequence
- accession: AY907566
- length: 1515
- database: ena
- NCBI tax ID: 384676
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pseudomonas entomophila L48 | GCA_000026105 | complete | ncbi | 384676 |
66792 | Pseudomonas entomophila L48 | 384676.9 | complete | patric | 384676 |
66792 | Pseudomonas entomophila L48 | 637000219 | complete | img | 384676 |
GC content
- @ref: 21139
- GC-content: 60
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 90.577 | no |
flagellated | yes | 84.067 | no |
gram-positive | no | 98.253 | no |
anaerobic | no | 98.834 | yes |
aerobic | yes | 96.038 | yes |
halophile | no | 86.739 | no |
spore-forming | no | 94.598 | no |
glucose-util | yes | 95.378 | yes |
thermophile | no | 99.847 | yes |
glucose-ferment | no | 88.175 | yes |
External links
@ref: 21139
culture collection no.: DSM 28517, CCUG 61470, CECT 7985
straininfo link
- @ref: 88315
- straininfo: 379920
literature
- topic: Phylogeny
- Pubmed-ID: 22326814
- title: Taxonomic characterisation of Pseudomonas strain L48 and formal proposal of Pseudomonas entomophila sp. nov.
- authors: Mulet M, Gomila M, Lemaitre B, Lalucat J, Garcia-Valdes E
- journal: Syst Appl Microbiol
- DOI: 10.1016/j.syapm.2011.12.003
- year: 2012
- mesh: Aerobiosis, Animals, Bacterial Typing Techniques, Catalase/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Drosophila melanogaster/microbiology, Flagella/physiology, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidoreductases/metabolism, Phylogeny, Pseudomonas/*classification/genetics/*isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- topic2: Metabolism
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
21139 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-28517 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28517) | |
62634 | Curators of the CCUG | https://www.ccug.se/strain?id=61470 | Culture Collection University of Gothenburg (CCUG) (CCUG 61470) | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
68369 | Automatically annotated from API 20NE | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
88315 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID379920.1 |