Strain identifier

BacDive ID: 24436

Type strain: Yes

Species: Pseudomonas entomophila

Strain Designation: L48

Strain history: <- CECT <- B. Lemaitre, Ecole Polytechnique Fédérale de Lausanne, Switzerland <- N. Vodovar

NCBI tax ID(s): 312306 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21139

BacDive-ID: 24436

DSM-Number: 28517

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, animal pathogen

description: Pseudomonas entomophila L48 is an aerobe, mesophilic animal pathogen that was isolated from Drosophila melanogaster.

NCBI tax id

  • NCBI tax id: 312306
  • Matching level: species

strain history

  • @ref: 21139
  • history: <- CECT <- B. Lemaitre, Ecole Polytechnique Fédérale de Lausanne, Switzerland <- N. Vodovar

doi: 10.13145/bacdive24436.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas entomophila
  • full scientific name: Pseudomonas entomophila Mulet et al. 2012

@ref: 21139

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas entomophila

full scientific name: Pseudomonas entomophila Mulet et al. 2012

strain designation: L48

type strain: yes

Morphology

multimedia

  • @ref: 21139
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_28517.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 21139
  • name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1a
  • composition: Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21139positivegrowth28mesophilic
62634positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 62634
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    62634C12:01.512
    62634C15:00.515
    62634C16:025.116
    62634C10:0 3OH9.411.423
    62634C12:0 2OH6.813.178
    62634C12:0 3OH513.455
    62634C16:1 ω7c14.715.819
    62634C16:1 ω7c DMA1.416.288
    62634C17:0 CYCLO13.116.888
    62634C18:1 ω7c /12t/9t12.217.824
    62634C18:1 ω9c0.917.769
    62634C18:2 ω6,9c/C18:0 ANTE1.317.724
    62634Unidentified0.49.395
    62634Unidentified1.19.983
    62634Unidentified1.610.473
    62634Unidentified4.410.651
    62634Unidentified0.711.196
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
21139---+-++-+-+++-++-++++
21139---+----+-+++-++-++++
21139---+--+-+-+++-++-++++

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentsampling date
21139Drosophila melanogasterDrosophila melanogasterCalvaire (Guadeloupe)FranceFRAEurope
62634DrosophilaGuadeloupe, CalvaireFranceFRAEurope2000-02-01

isolation source categories

  • Cat1: #Host
  • Cat2: #Arthropoda
  • Cat3: #Insecta

taxonmaps

  • @ref: 69479
  • File name: preview.99_239.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_203;99_239&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AY907566
  • Sequence Identity:
  • Total samples: 2133
  • soil counts: 368
  • aquatic counts: 543
  • animal counts: 799
  • plant counts: 423

Safety information

risk assessment

  • @ref: 21139
  • pathogenicity animal: yes
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21139
  • description: Pseudomonas entomophila strain L48 16S ribosomal RNA gene, partial sequence
  • accession: AY907566
  • length: 1515
  • database: ena
  • NCBI tax ID: 384676

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas entomophila L48GCA_000026105completencbi384676
66792Pseudomonas entomophila L48384676.9completepatric384676
66792Pseudomonas entomophila L48637000219completeimg384676

GC content

  • @ref: 21139
  • GC-content: 60

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.577no
flagellatedyes84.067no
gram-positiveno98.253no
anaerobicno98.834yes
aerobicyes96.038yes
halophileno86.739no
spore-formingno94.598no
glucose-utilyes95.378yes
thermophileno99.847yes
glucose-fermentno88.175yes

External links

@ref: 21139

culture collection no.: DSM 28517, CCUG 61470, CECT 7985

straininfo link

  • @ref: 88315
  • straininfo: 379920

literature

  • topic: Phylogeny
  • Pubmed-ID: 22326814
  • title: Taxonomic characterisation of Pseudomonas strain L48 and formal proposal of Pseudomonas entomophila sp. nov.
  • authors: Mulet M, Gomila M, Lemaitre B, Lalucat J, Garcia-Valdes E
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/j.syapm.2011.12.003
  • year: 2012
  • mesh: Aerobiosis, Animals, Bacterial Typing Techniques, Catalase/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Drosophila melanogaster/microbiology, Flagella/physiology, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidoreductases/metabolism, Phylogeny, Pseudomonas/*classification/genetics/*isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21139Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28517Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28517)
62634Curators of the CCUGhttps://www.ccug.se/strain?id=61470Culture Collection University of Gothenburg (CCUG) (CCUG 61470)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88315Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID379920.1