Strain identifier
BacDive ID: 24360
Type strain:
Species: Pseudomonas aeruginosa
Strain Designation: 1435, C10
Strain history: CIP <- 1995, ATCC <- L. Dicksinson <- Boots Pure Drug Co Ldt: strain C10
NCBI tax ID(s): 287 (species)
General
@ref: 21109
BacDive-ID: 24360
DSM-Number: 50073
keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen
description: Pseudomonas aeruginosa 1435 is an obligate aerobe, mesophilic, Gram-negative human pathogen of the family Pseudomonadaceae.
NCBI tax id
- NCBI tax id: 287
- Matching level: species
strain history
@ref | history |
---|---|
21109 | <- ICPB <- ATCC <- L. Dickinson <- Boots Pure Drug, Co. Ltd. C10 |
67770 | K. Komagata <-- R. Hugh 1435. |
116084 | CIP <- 1995, ATCC <- L. Dicksinson <- Boots Pure Drug Co Ldt: strain C10 |
doi: 10.13145/bacdive24360.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas aeruginosa
- full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacterium aeruginosum
@ref: 21109
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas aeruginosa
full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900
strain designation: 1435, C10
type strain: no
Morphology
cell morphology
- @ref: 116084
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 116084
- production: yes
- name: Pyocyanin
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
21109 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
35680 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
116084 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
21109 | positive | growth | 37 | mesophilic |
35680 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
116084 | positive | growth | 30-41 | |
116084 | no | growth | 5 | psychrophilic |
116084 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 116084
- oxygen tolerance: obligate aerobe
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | + | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | + | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | + | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | + | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | + | reduction |
116084 | 16947 | citrate | + | carbon source |
116084 | 4853 | esculin | - | hydrolysis |
116084 | 17632 | nitrate | + | reduction |
116084 | 16301 | nitrite | + | reduction |
116084 | 17632 | nitrate | + | respiration |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
116084 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | indole test | voges-proskauer-test |
---|---|---|---|---|
68369 | 35581 | indole | - | |
116084 | 15688 | acetoin | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | + | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | + | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
116084 | oxidase | + | |
116084 | beta-galactosidase | - | 3.2.1.23 |
116084 | alcohol dehydrogenase | + | 1.1.1.1 |
116084 | gelatinase | + | |
116084 | amylase | - | |
116084 | DNase | + | |
116084 | caseinase | + | 3.4.21.50 |
116084 | tween esterase | + | |
116084 | lecithinase | + | |
116084 | lipase | + | |
116084 | lysine decarboxylase | - | 4.1.1.18 |
116084 | ornithine decarboxylase | - | 4.1.1.17 |
116084 | tryptophan deaminase | - | |
116084 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116084 | - | + | + | + | + | + | - | - | + | - | + | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
21109 | + | - | - | + | + | - | + | - | + | - | - | + | + | - | + | + | + | + | + | - | + |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116084 | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | + | - | - | - | - | + | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | + | - | - | + | - | - | + | - | - | + | + | - | + | + | + | + | - | + | - | - | - | + | + | + | + | + | + | + | - | + | + | + | + | + | + | - | - | + | + | + | + | + | + | + | + | + | + | + | + |
Isolation, sampling and environmental information
taxonmaps
- @ref: 69479
- File name: preview.99_42.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_36;97_37;98_40;99_42&stattab=map
- Last taxonomy: Pseudomonas aeruginosa
- 16S sequence: LC508775
- Sequence Identity:
- Total samples: 22757
- soil counts: 1669
- aquatic counts: 5046
- animal counts: 14748
- plant counts: 1294
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
21109 | yes | yes | 2 | Risk group (German classification) |
116084 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 67770
- description: Pseudomonas aeruginosa JCM 14176 gene for 16S ribosomal RNA, partial sequence
- accession: LC508775
- length: 1458
- database: ena
- NCBI tax ID: 287
External links
@ref: 21109
culture collection no.: DSM 50073, ATCC 12055, ICPB 2603, JCM 14176, BCRC 16043, CIP 104467
straininfo link
- @ref: 88284
- straininfo: 35492
literature
- topic: Metabolism
- Pubmed-ID: 26117599
- title: Direct measurement of efflux in Pseudomonas aeruginosa using an environment-sensitive fluorescent dye.
- authors: Iyer R, Erwin AL
- journal: Res Microbiol
- DOI: 10.1016/j.resmic.2015.06.006
- year: 2015
- mesh: Anti-Bacterial Agents/metabolism, Bacterial Proteins/*metabolism, Drug Discovery/*methods, Fluorescent Dyes/metabolism, *Genes, MDR, Glucose/metabolism, Malates/metabolism, Oxazines/*metabolism, Pseudomonas aeruginosa/*metabolism
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
21109 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-50073 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 50073) | |
35680 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/16379 | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
68369 | Automatically annotated from API 20NE | |||
68382 | Automatically annotated from API zym | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
88284 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID35492.1 | |
116084 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104467 | Collection of Institut Pasteur (CIP 104467) |