Strain identifier

BacDive ID: 24337

Type strain: No

Species: Porphyromonas gingivalis

Strain Designation: HG66

Strain history: <- J. Potempa <- R. Arnold, Emory University; HG66

NCBI tax ID(s): 837 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 20739

BacDive-ID: 24337

DSM-Number: 28984

keywords: genome sequence, Bacteria, anaerobe, mesophilic, human pathogen

description: Porphyromonas gingivalis HG66 is an anaerobe, mesophilic human pathogen that was isolated from human oral cavity.

NCBI tax id

  • NCBI tax id: 837
  • Matching level: species

strain history

  • @ref: 20739
  • history: <- J. Potempa <- R. Arnold, Emory University; HG66

doi: 10.13145/bacdive24337.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Porphyromonadaceae
  • genus: Porphyromonas
  • species: Porphyromonas gingivalis
  • full scientific name: Porphyromonas gingivalis (Coykendall et al. 1980) Shah and Collins 1988

  • synonyms

    • @ref: 20215
    • synonym: Bacteroides gingivalis

@ref: 20739

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Porphyromonadaceae

genus: Porphyromonas

species: Porphyromonas gingivalis

full scientific name: Porphyromonas gingivalis (Coykendall et al. 1980) Shah and Collins 1988 emend. Hahnke et al. 2016

strain designation: HG66

type strain: no

Morphology

colony morphology

  • @ref: 20739
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
20739COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
20739CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)yeshttps://mediadive.dsmz.de/medium/110Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water

culture temp

  • @ref: 20739
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 20739
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan+energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68380serine arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase+4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380alpha-galactosidase-3.2.1.22
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
20739---++/-----+-----++--+--+/--+--+/--
20739-+-++/-----+-----++--+--+/--+--+-
20739---+/------+-----++--+----+--+-

Isolation, sampling and environmental information

isolation

  • @ref: 20739
  • sample type: human oral cavity
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways
#Host Body-Site#Oral cavity and airways#Mouth

Safety information

risk assessment

  • @ref: 20739
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Porphyromonas gingivalis HG66GCA_000739415chromosomencbi837
66792Porphyromonas gingivalis HG662597490155draftimg837

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno90.794no
flagellatedno96.703no
gram-positiveno97.387no
anaerobicyes99.34yes
aerobicno98.605yes
halophileno81.037no
spore-formingno95.937no
glucose-utilyes80.841no
glucose-fermentno52.679no
thermophileno97.638yes

External links

@ref: 20739

culture collection no.: DSM 28984

straininfo link

  • @ref: 88263
  • straininfo: 407947

literature

  • topic: Genetics
  • Pubmed-ID: 25291768
  • title: Genome Sequence of Porphyromonas gingivalis Strain HG66 (DSM 28984).
  • authors: Siddiqui H, Yoder-Himes DR, Mizgalska D, Nguyen KA, Potempa J, Olsen I
  • journal: Genome Announc
  • DOI: 10.1128/genomeA.00947-14
  • year: 2014

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20739Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28984Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28984)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68380Automatically annotated from API rID32A
88263Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407947.1