Strain identifier
BacDive ID: 24298
Type strain:
Species: Protochlamydia amoebophila
Strain Designation: UWE25
Strain history: <- M. Horn, Univ. Vienna, Dept. Microbial Ecol., Austria; UWE25 <- T. R. Fritsche, Univ. of Washington, Dept. of Lab. Medicine
NCBI tax ID(s): 362787 (species)
General
@ref: 19360
BacDive-ID: 24298
DSM-Number: 27361
keywords: 16S sequence, Bacteria, mesophilic
description: Protochlamydia amoebophila UWE25 is a mesophilic bacterium that was isolated from natural bacterial endosymbiont of an Acanthamoeba sp. isolated from soil.
NCBI tax id
- NCBI tax id: 362787
- Matching level: species
strain history
- @ref: 19360
- history: <- M. Horn, Univ. Vienna, Dept. Microbial Ecol., Austria; UWE25 <- T. R. Fritsche, Univ. of Washington, Dept. of Lab. Medicine
doi: 10.13145/bacdive24298.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/chlamydiota
- domain: Bacteria
- phylum: Chlamydiota
- class: Chlamydiia
- order: Chlamydiales
- family: Parachlamydiaceae
- genus: Protochlamydia
- species: Protochlamydia amoebophila
- full scientific name: Candidatus Protochlamydia amoebophila Collingro et al. 2005
@ref: 19360
domain: Bacteria
phylum: Chlamydiae
class: Chlamydiia
order: Chlamydiales
family: Parachlamydiaceae
genus: Candidatus Protochlamydia
species: Candidatus Protochlamydia amoebophila
full scientific name: Candidatus Protochlamydia amoebophila Collingro et al. 2005
strain designation: UWE25
type strain: yes
Morphology
colony morphology
- @ref: 19360
- incubation period: 2-3 days
Culture and growth conditions
culture medium
- @ref: 19360
- name: ACANTHAMOEBA MEDIUM (DSMZ Medium 1500)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1500
- composition: Name: ACANTHAMOEBA MEDIUM (DSMZ Medium 1500) Composition: Trypticase soy broth 30.0 g/l Proteose peptone 20.0 g/l Glucose 18.0 g/l Yeast extract 2.0 g/l Sodium citrate dihydrate 1.0 g/l MgSO4 x 7 H2O 0.98 g/l Na2HPO4 x 7 H2O 0.355 g/l KH2PO4 0.34 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.02 g/l Distilled water
culture temp
- @ref: 19360
- growth: positive
- type: growth
- temperature: 25
- range: mesophilic
Isolation, sampling and environmental information
isolation
- @ref: 19360
- sample type: natural bacterial endosymbiont of an Acanthamoeba sp. isolated from soil
- host species: Acanthamoeba
- geographic location: western Washington State
- country: USA
- origin.country: USA
- continent: North America
- latitude: 47.6
- longitude: -122.317
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Host | #Other | #Endosymbiont |
#Host | #Microbial | #Bacteria |
Sequence information
16S sequences
- @ref: 19360
- description: Candidatus Protochlamydia amoebophila UWE25 strain UWE25 16S ribosomal RNA, complete sequence
- accession: NR_074271
- length: 1481
- database: nuccore
- NCBI tax ID: 264201
External links
@ref: 19360
culture collection no.: DSM 27361, ATCC PRA-7
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 15615594 | A genomic island present along the bacterial chromosome of the Parachlamydiaceae UWE25, an obligate amoebal endosymbiont, encodes a potentially functional F-like conjugative DNA transfer system. | Greub G, Collyn F, Guy L, Roten CA | BMC Microbiol | 10.1186/1471-2180-4-48 | 2004 | Acanthamoeba/microbiology, Animals, Base Composition/genetics, Chlamydiales/classification/*genetics, Chromosomes, Bacterial/*genetics, Computational Biology/methods, Conjugation, Genetic/*genetics, DNA, Bacterial/genetics, Gene Order/genetics, Genomic Islands/*genetics, Operon/*genetics, Phylogeny, Symbiosis | Genetics |
Genetics | 16703114 | Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens. | Ogata H, La Scola B, Audic S, Renesto P, Blanc G, Robert C, Fournier PE, Claverie JM, Raoult D | PLoS Genet | 10.1371/journal.pgen.0020076 | 2006 | Actins/metabolism, Amoeba/*genetics/*microbiology, Animals, Chlorocebus aethiops, Cluster Analysis, *Genome, Bacterial, *Models, Genetic, Multigene Family, Open Reading Frames, Phylogeny, Rickettsia/*genetics/*pathogenicity, Rickettsia Infections/genetics, Vero Cells | Phylogeny |
Metabolism | 16796686 | Tapping the nucleotide pool of the host: novel nucleotide carrier proteins of Protochlamydia amoebophila. | Haferkamp I, Schmitz-Esser S, Wagner M, Neigel N, Horn M, Neuhaus HE | Mol Microbiol | 10.1111/j.1365-2958.2006.05193.x | 2006 | Adenosine Triphosphate/metabolism, Amino Acid Sequence, Bacterial Proteins/chemistry/genetics/*metabolism, Biological Transport, Chlamydia/genetics/*metabolism, Genes, Bacterial/genetics, Guanosine Triphosphate/metabolism, Molecular Sequence Data, Nucleotide Transport Proteins/chemistry/genetics/*metabolism, Nucleotides/*metabolism, Protein Structure, Secondary, Substrate Specificity, Transcription, Genetic, Uridine Triphosphate/metabolism | Genetics |
Metabolism | 17760504 | Enlightening energy parasitism by analysis of an ATP/ADP transporter from chlamydiae. | Trentmann O, Horn M, van Scheltinga AC, Neuhaus HE, Haferkamp I | PLoS Biol | 10.1371/journal.pbio.0050231 | 2007 | Adenosine Triphosphate/*metabolism, Bacterial Proteins, Chlamydia/*metabolism/pathogenicity, *Energy Metabolism, Kinetics, Liposomes, Membrane Transport Proteins, Nucleotide Transport Proteins/isolation & purification/*metabolism | Enzymology |
18021397 | Analyses of six homologous proteins of Protochlamydia amoebophila UWE25 encoded by large GC-rich genes (lgr): a model of evolution and concatenation of leucine-rich repeats. | Eugster M, Roten CA, Greub G | BMC Evol Biol | 10.1186/1471-2148-7-231 | 2007 | Amino Acid Motifs, Bacterial Proteins/genetics, Chlamydiales/*genetics, *Evolution, Molecular, *GC Rich Sequence, *Genes, Bacterial, Genomic Islands, Leucine/*genetics, Leucine-Rich Repeat Proteins, Proteins/*genetics, Repetitive Sequences, Amino Acid, Sequence Alignment, Sequence Homology, Amino Acid | ||
18493612 | Chlamydiae has contributed at least 55 genes to Plantae with predominantly plastid functions. | Moustafa A, Reyes-Prieto A, Bhattacharya D | PLoS One | 10.1371/journal.pone.0002205 | 2008 | Chlamydia/*genetics, *Genes, Bacterial, Plants/genetics/*microbiology, *Plastids | ||
Metabolism | 20675479 | Inclusion membrane proteins of Protochlamydia amoebophila UWE25 reveal a conserved mechanism for host cell interaction among the Chlamydiae. | Heinz E, Rockey DD, Montanaro J, Aistleitner K, Wagner M, Horn M | J Bacteriol | 10.1128/JB.00605-10 | 2010 | Acanthamoeba castellanii/microbiology, Animals, Bacterial Proteins/genetics/*metabolism, Blotting, Western, Chlamydia/genetics/*growth & development/*metabolism/ultrastructure, Membrane Proteins/genetics/*metabolism, Microscopy, Immunoelectron | Pathogenicity |
Metabolism | 21500343 | Proteomic analysis reveals a virtually complete set of proteins for translation and energy generation in elementary bodies of the amoeba symbiont Protochlamydia amoebophila. | Sixt BS, Heinz C, Pichler P, Heinz E, Montanaro J, Op den Camp HJ, Ammerer G, Mechtler K, Wagner M, Horn M | Proteomics | 10.1002/pmic.201000510 | 2011 | Acanthamoeba/*microbiology, Bacterial Proteins/genetics/*metabolism, Chlamydiales/genetics/*metabolism/physiology, Electrophoresis, Gel, Two-Dimensional, Energy Metabolism, Metabolic Networks and Pathways, Microscopy, Electron, Transmission, Peptide Mapping, Protein Biosynthesis, Proteome/*chemistry, Proteomics, Symbiosis/physiology, Transcription, Genetic | Enzymology |
Metabolism | 23708130 | Chlamydia trachomatis transports NAD via the Npt1 ATP/ADP translocase. | Fisher DJ, Fernandez RE, Maurelli AT | J Bacteriol | 10.1128/JB.00433-13 | 2013 | Bacterial Proteins/genetics/*metabolism, Chlamydia trachomatis/*enzymology/genetics/*metabolism, Escherichia coli/genetics/metabolism, Gene Expression, Isotope Labeling, Kinetics, Membrane Transport Proteins/genetics/*metabolism, Mitochondrial ADP, ATP Translocases/genetics/*metabolism, Models, Biological, NAD/*metabolism, Phosphorus Radioisotopes/metabolism, Substrate Specificity | Enzymology |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
19360 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27361) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-27361 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ |