Strain identifier

BacDive ID: 24298

Type strain: Yes

Species: Protochlamydia amoebophila

Strain Designation: UWE25

Strain history: <- M. Horn, Univ. Vienna, Dept. Microbial Ecol., Austria; UWE25 <- T. R. Fritsche, Univ. of Washington, Dept. of Lab. Medicine

NCBI tax ID(s): 362787 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 19360

BacDive-ID: 24298

DSM-Number: 27361

keywords: 16S sequence, Bacteria, mesophilic

description: Protochlamydia amoebophila UWE25 is a mesophilic bacterium that was isolated from natural bacterial endosymbiont of an Acanthamoeba sp. isolated from soil.

NCBI tax id

  • NCBI tax id: 362787
  • Matching level: species

strain history

  • @ref: 19360
  • history: <- M. Horn, Univ. Vienna, Dept. Microbial Ecol., Austria; UWE25 <- T. R. Fritsche, Univ. of Washington, Dept. of Lab. Medicine

doi: 10.13145/bacdive24298.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/chlamydiota
  • domain: Bacteria
  • phylum: Chlamydiota
  • class: Chlamydiia
  • order: Chlamydiales
  • family: Parachlamydiaceae
  • genus: Protochlamydia
  • species: Protochlamydia amoebophila
  • full scientific name: Candidatus Protochlamydia amoebophila Collingro et al. 2005

@ref: 19360

domain: Bacteria

phylum: Chlamydiae

class: Chlamydiia

order: Chlamydiales

family: Parachlamydiaceae

genus: Candidatus Protochlamydia

species: Candidatus Protochlamydia amoebophila

full scientific name: Candidatus Protochlamydia amoebophila Collingro et al. 2005

strain designation: UWE25

type strain: yes

Morphology

colony morphology

  • @ref: 19360
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 19360
  • name: ACANTHAMOEBA MEDIUM (DSMZ Medium 1500)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1500
  • composition: Name: ACANTHAMOEBA MEDIUM (DSMZ Medium 1500) Composition: Trypticase soy broth 30.0 g/l Proteose peptone 20.0 g/l Glucose 18.0 g/l Yeast extract 2.0 g/l Sodium citrate dihydrate 1.0 g/l MgSO4 x 7 H2O 0.98 g/l Na2HPO4 x 7 H2O 0.355 g/l KH2PO4 0.34 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.02 g/l Distilled water

culture temp

  • @ref: 19360
  • growth: positive
  • type: growth
  • temperature: 25
  • range: mesophilic

Isolation, sampling and environmental information

isolation

  • @ref: 19360
  • sample type: natural bacterial endosymbiont of an Acanthamoeba sp. isolated from soil
  • host species: Acanthamoeba
  • geographic location: western Washington State
  • country: USA
  • origin.country: USA
  • continent: North America
  • latitude: 47.6
  • longitude: -122.317

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Other#Endosymbiont
#Host#Microbial#Bacteria

Sequence information

16S sequences

  • @ref: 19360
  • description: Candidatus Protochlamydia amoebophila UWE25 strain UWE25 16S ribosomal RNA, complete sequence
  • accession: NR_074271
  • length: 1481
  • database: nuccore
  • NCBI tax ID: 264201

External links

@ref: 19360

culture collection no.: DSM 27361, ATCC PRA-7

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15615594A genomic island present along the bacterial chromosome of the Parachlamydiaceae UWE25, an obligate amoebal endosymbiont, encodes a potentially functional F-like conjugative DNA transfer system.Greub G, Collyn F, Guy L, Roten CABMC Microbiol10.1186/1471-2180-4-482004Acanthamoeba/microbiology, Animals, Base Composition/genetics, Chlamydiales/classification/*genetics, Chromosomes, Bacterial/*genetics, Computational Biology/methods, Conjugation, Genetic/*genetics, DNA, Bacterial/genetics, Gene Order/genetics, Genomic Islands/*genetics, Operon/*genetics, Phylogeny, SymbiosisGenetics
Genetics16703114Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens.Ogata H, La Scola B, Audic S, Renesto P, Blanc G, Robert C, Fournier PE, Claverie JM, Raoult DPLoS Genet10.1371/journal.pgen.00200762006Actins/metabolism, Amoeba/*genetics/*microbiology, Animals, Chlorocebus aethiops, Cluster Analysis, *Genome, Bacterial, *Models, Genetic, Multigene Family, Open Reading Frames, Phylogeny, Rickettsia/*genetics/*pathogenicity, Rickettsia Infections/genetics, Vero CellsPhylogeny
Metabolism16796686Tapping the nucleotide pool of the host: novel nucleotide carrier proteins of Protochlamydia amoebophila.Haferkamp I, Schmitz-Esser S, Wagner M, Neigel N, Horn M, Neuhaus HEMol Microbiol10.1111/j.1365-2958.2006.05193.x2006Adenosine Triphosphate/metabolism, Amino Acid Sequence, Bacterial Proteins/chemistry/genetics/*metabolism, Biological Transport, Chlamydia/genetics/*metabolism, Genes, Bacterial/genetics, Guanosine Triphosphate/metabolism, Molecular Sequence Data, Nucleotide Transport Proteins/chemistry/genetics/*metabolism, Nucleotides/*metabolism, Protein Structure, Secondary, Substrate Specificity, Transcription, Genetic, Uridine Triphosphate/metabolismGenetics
Metabolism17760504Enlightening energy parasitism by analysis of an ATP/ADP transporter from chlamydiae.Trentmann O, Horn M, van Scheltinga AC, Neuhaus HE, Haferkamp IPLoS Biol10.1371/journal.pbio.00502312007Adenosine Triphosphate/*metabolism, Bacterial Proteins, Chlamydia/*metabolism/pathogenicity, *Energy Metabolism, Kinetics, Liposomes, Membrane Transport Proteins, Nucleotide Transport Proteins/isolation & purification/*metabolismEnzymology
18021397Analyses of six homologous proteins of Protochlamydia amoebophila UWE25 encoded by large GC-rich genes (lgr): a model of evolution and concatenation of leucine-rich repeats.Eugster M, Roten CA, Greub GBMC Evol Biol10.1186/1471-2148-7-2312007Amino Acid Motifs, Bacterial Proteins/genetics, Chlamydiales/*genetics, *Evolution, Molecular, *GC Rich Sequence, *Genes, Bacterial, Genomic Islands, Leucine/*genetics, Leucine-Rich Repeat Proteins, Proteins/*genetics, Repetitive Sequences, Amino Acid, Sequence Alignment, Sequence Homology, Amino Acid
18493612Chlamydiae has contributed at least 55 genes to Plantae with predominantly plastid functions.Moustafa A, Reyes-Prieto A, Bhattacharya DPLoS One10.1371/journal.pone.00022052008Chlamydia/*genetics, *Genes, Bacterial, Plants/genetics/*microbiology, *Plastids
Metabolism20675479Inclusion membrane proteins of Protochlamydia amoebophila UWE25 reveal a conserved mechanism for host cell interaction among the Chlamydiae.Heinz E, Rockey DD, Montanaro J, Aistleitner K, Wagner M, Horn MJ Bacteriol10.1128/JB.00605-102010Acanthamoeba castellanii/microbiology, Animals, Bacterial Proteins/genetics/*metabolism, Blotting, Western, Chlamydia/genetics/*growth & development/*metabolism/ultrastructure, Membrane Proteins/genetics/*metabolism, Microscopy, ImmunoelectronPathogenicity
Metabolism21500343Proteomic analysis reveals a virtually complete set of proteins for translation and energy generation in elementary bodies of the amoeba symbiont Protochlamydia amoebophila.Sixt BS, Heinz C, Pichler P, Heinz E, Montanaro J, Op den Camp HJ, Ammerer G, Mechtler K, Wagner M, Horn MProteomics10.1002/pmic.2010005102011Acanthamoeba/*microbiology, Bacterial Proteins/genetics/*metabolism, Chlamydiales/genetics/*metabolism/physiology, Electrophoresis, Gel, Two-Dimensional, Energy Metabolism, Metabolic Networks and Pathways, Microscopy, Electron, Transmission, Peptide Mapping, Protein Biosynthesis, Proteome/*chemistry, Proteomics, Symbiosis/physiology, Transcription, GeneticEnzymology
Metabolism23708130Chlamydia trachomatis transports NAD via the Npt1 ATP/ADP translocase.Fisher DJ, Fernandez RE, Maurelli ATJ Bacteriol10.1128/JB.00433-132013Bacterial Proteins/genetics/*metabolism, Chlamydia trachomatis/*enzymology/genetics/*metabolism, Escherichia coli/genetics/metabolism, Gene Expression, Isotope Labeling, Kinetics, Membrane Transport Proteins/genetics/*metabolism, Mitochondrial ADP, ATP Translocases/genetics/*metabolism, Models, Biological, NAD/*metabolism, Phosphorus Radioisotopes/metabolism, Substrate SpecificityEnzymology

Reference

@idauthorscataloguedoi/urltitle
19360Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27361)https://www.dsmz.de/collection/catalogue/details/culture/DSM-27361
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ