Strain identifier

BacDive ID: 24238

Type strain: Yes

Species: Mycobacterium bourgelatii

Strain Designation: 02-5273-A84, MLB-A84, MLB-A84 (5273)

Strain history: CIP <- 2012, B. Lamy, Montpellier Hosp., Montpellier, France: strain MLB-A84 (5273)

NCBI tax ID(s): 1273442 (species)

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General

@ref: 20673

BacDive-ID: 24238

DSM-Number: 45746

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-positive, rod-shaped, animal pathogen

description: Mycobacterium bourgelatii 02-5273-A84 is a mesophilic, Gram-positive, rod-shaped animal pathogen that was isolated from bovine lymph nodes.

NCBI tax id

  • NCBI tax id: 1273442
  • Matching level: species

strain history

@refhistory
20673<- V. Guérin-Faublée, VetAgro-Sup Campus de Lyon, France; MLB-A84
67770CIP 110557 <-- B. Lamy; Montpellier Hosp., France; MLB-A84.
116901CIP <- 2012, B. Lamy, Montpellier Hosp., Montpellier, France: strain MLB-A84 (5273)

doi: 10.13145/bacdive24238.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium bourgelatii
  • full scientific name: Mycobacterium bourgelatii Guérin-Faublée et al. 2013

@ref: 20673

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium bourgelatii

full scientific name: Mycobacterium bourgelatii Guérin-Faublée et al. 2013

strain designation: 02-5273-A84, MLB-A84, MLB-A84 (5273)

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
31096positiverod-shaped
69480no91.311
69480positive100
116901rod-shapedno

pigmentation

  • @ref: 31096
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
41221MEDIUM 55 - for Mycobacteriumyes
20673MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
116901CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
20673positivegrowth37mesophilic
31096positivegrowth20-42
31096positiveoptimum42thermophilic
41221positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.748

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836927689decanoate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
31096arylsulfatase+3.1.6.1
31096catalase+1.11.1.6
68369cytochrome oxidase-1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase-3.1.3.1
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20673--+/-++/-+----+---------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
20673------+-------+/--+/-----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
20673bovine lymph nodesEureFranceFRAEurope
67770Bovine lymph nodesFranceFRAEurope
116901Animal, BovineEureFranceFRAEurope2002

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Organ#Lymph node

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
20673yes, in single cases1Risk group (German classification)
1169011Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20673
  • description: Mycobacterium bourgelatii partial 16S rRNA gene, isolate 02-5273-A84
  • accession: HF674384
  • length: 1284
  • database: ena
  • NCBI tax ID: 1273442

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium bourgelatii JCM 30725GCA_010723575contigncbi1273442
66792Mycobacterium bourgelatii strain JCM 307251273442.8wgspatric1273442
66792Mycobacterium bourgelatii DSM 45746GCA_025821955scaffoldncbi1273442

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno74no
motileno94.979no
gram-positiveyes89.666yes
anaerobicno98.868no
aerobicyes90.433no
halophileno90.961no
spore-formingno78.046no
glucose-utilyes62.201yes
flagellatedno98.937no
thermophileno98.121no
glucose-fermentno92.881yes

External links

@ref: 20673

culture collection no.: DSM 45746, CIP 110557, JCM 30725

straininfo link

  • @ref: 88181
  • straininfo: 391579

literature

  • topic: Phylogeny
  • Pubmed-ID: 23990648
  • title: Mycobacterium bourgelatii sp. nov., a rapidly growing, non-chromogenic species isolated from the lymph nodes of cattle.
  • authors: Guerin-Faublee V, Flandrois JP, Pichat C, Boschiroli ML, Lamy B
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.051979-0
  • year: 2013
  • mesh: Animals, Bacterial Typing Techniques, Cattle/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, France, Genes, Bacterial, Lymph Nodes/*microbiology, Molecular Sequence Data, Mycobacterium/*classification/genetics/isolation & purification, Mycolic Acids/chemistry, *Phylogeny, RNA, Bacterial/genetics, Ribosome Subunits, Small, Bacterial/genetics
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20673Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-45746Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45746)
31096Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172742528776041
41221Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8429
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
88181Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID391579.1
116901Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110557Collection of Institut Pasteur (CIP 110557)