Strain identifier
BacDive ID: 2422
Type strain: 
Species: Chloroflexus aggregans
Strain Designation: YI-9
Strain history: <- S. Hanada, YI-9
NCBI tax ID(s): 152260 (species)
version 8.1 (current version)
General
@ref: 3608
BacDive-ID: 2422
DSM-Number: 9486
keywords: 16S sequence, Bacteria, anaerobe, thermophilic
description: Chloroflexus aggregans YI-9 is an anaerobe, thermophilic bacterium that was isolated from Yufuin hot spring.
NCBI tax id
- NCBI tax id: 152260
- Matching level: species
strain history
- @ref: 3608
- history: <- S. Hanada, YI-9
doi: 10.13145/bacdive2422.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/chloroflexota
- domain: Bacteria
- phylum: Chloroflexota
- class: Chloroflexia
- order: Chloroflexales
- family: Chloroflexaceae
- genus: Chloroflexus
- species: Chloroflexus aggregans
- full scientific name: Chloroflexus aggregans Hanada et al. 1995
@ref: 3608
domain: Bacteria
phylum: Chloroflexi
class: Chloroflexia
order: Chloroflexales, not validated
family: Chloroflexaceae
genus: Chloroflexus
species: Chloroflexus aggregans
full scientific name: Chloroflexus aggregans Hanada et al. 1995
strain designation: YI-9
type strain: no
Culture and growth conditions
culture medium
- @ref: 3608
- name: CHLOROFLEXUS AGGREGANS MEDIUM (DSMZ Medium 87a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/87a
- composition: Name: CHLOROFLEXUS AGGREGANS MEDIUM (DSMZ Medium 87a) Composition: Glycyl-glycine 0.952381 g/l Yeast extract 0.952381 g/l NaNO3 0.47619 g/l NaCl 0.0952381 g/l KNO3 0.0952381 g/l MgSO4 x 7 H2O 0.0952381 g/l Na2HPO4 x 2 H2O 0.0952381 g/l CaCl2 x 2 H2O 0.047619 g/l Na3-EDTA 0.00190476 g/l Nicotinic acid 0.000952381 g/l Thiamine-HCl x 2 H2O 0.000952381 g/l Folic acid 0.00047619 g/l Pyridoxine hydrochloride 0.00047619 g/l Calcium pantothenate 0.00047619 g/l p-Aminobenzoic acid 0.00047619 g/l H3BO3 0.000285714 g/l CoCl2 x 6 H2O 0.000190476 g/l ZnSO4 x 7 H2O 9.52381e-05 g/l Biotin 4.7619e-05 g/l Na2MoO4 x 2 H2O 2.85714e-05 g/l MnCl2 x 4 H2O 2.85714e-05 g/l NiCl2 x 6 H2O 1.90476e-05 g/l Vitamin B12 9.52381e-06 g/l CuCl2 x 2 H2O 9.52381e-06 g/l Fe(III) citrate Distilled water
culture temp
- @ref: 3608
- growth: positive
- type: growth
- temperature: 55
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 3608
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 3608
- sample type: Yufuin hot spring
- geographic location: Oh-Ita prefecture
- country: Japan
- origin.country: JPN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_5628.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_477;96_2708;97_3327;98_4194;99_5628&stattab=map
- Last taxonomy: Chloroflexus aggregans subclade
- 16S sequence: AJ308499
- Sequence Identity:
- Total samples: 100
- soil counts: 5
- aquatic counts: 90
- animal counts: 5
Safety information
risk assessment
- @ref: 3608
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Chloroflexus aggregans partial 16S rRNA gene, strain DSM 9486 | AJ308499 | 1355 | ena | 152260 |
| 3608 | Chloroflexus aggregans DSM 9485, complete genome | CP001337 | 4684931 | ena | 326427 |
GC content
- @ref: 3608
- GC-content: 56.7-57
- method: high performance liquid chromatography (HPLC)
External links
@ref: 3608
culture collection no.: DSM 9486
straininfo link
- @ref: 71972
- straininfo: 48637
literature
- topic: Phylogeny
- Pubmed-ID: 7547286
- title: Chloroflexus aggregans sp. nov., a filamentous phototrophic bacterium which forms dense cell aggregates by active gliding movement.
- authors: Hanada S, Hiraishi A, Shimada K, Matsuura K
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-45-4-676
- year: 1995
- mesh: Bacteria/*classification/metabolism, Base Sequence, DNA, Bacterial/analysis, Molecular Sequence Data, *Photosynthesis
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 3608 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9486) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-9486 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 71972 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48637.1 | StrainInfo: A central database for resolving microbial strain identifiers |