Strain identifier

BacDive ID: 2421

Type strain: Yes

Species: Chloroflexus aggregans

Strain Designation: MD-66

Strain history: <- S. Hanada, MD-66

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 3599

BacDive-ID: 2421

DSM-Number: 9485

keywords: genome sequence, Bacteria, anaerobe, photoheterotroph, thermophilic

description: Chloroflexus aggregans MD-66 is an anaerobe, photoheterotroph, thermophilic bacterium that was isolated from Meotobuchi hot spring.

NCBI tax id

NCBI tax idMatching level
152260species
326427strain

strain history

  • @ref: 3599
  • history: <- S. Hanada, MD-66

doi: 10.13145/bacdive2421.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/chloroflexota
  • domain: Bacteria
  • phylum: Chloroflexota
  • class: Chloroflexia
  • order: Chloroflexales
  • family: Chloroflexaceae
  • genus: Chloroflexus
  • species: Chloroflexus aggregans
  • full scientific name: Chloroflexus aggregans Hanada et al. 1995

@ref: 3599

domain: Bacteria

phylum: Chloroflexi

class: Chloroflexia

order: Chloroflexales, not validated

family: Chloroflexaceae

genus: Chloroflexus

species: Chloroflexus aggregans

full scientific name: Chloroflexus aggregans Hanada et al. 1995

strain designation: MD-66

type strain: yes

Morphology

cell morphology

@refcell lengthgram stainconfidence
229071-1.5 µm
69480positive94.131

Culture and growth conditions

culture medium

  • @ref: 3599
  • name: CHLOROFLEXUS AGGREGANS MEDIUM (DSMZ Medium 87a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/87a
  • composition: Name: CHLOROFLEXUS AGGREGANS MEDIUM (DSMZ Medium 87a) Composition: Glycyl-glycine 0.952381 g/l Yeast extract 0.952381 g/l NaNO3 0.47619 g/l NaCl 0.0952381 g/l KNO3 0.0952381 g/l MgSO4 x 7 H2O 0.0952381 g/l Na2HPO4 x 2 H2O 0.0952381 g/l CaCl2 x 2 H2O 0.047619 g/l Na3-EDTA 0.00190476 g/l Nicotinic acid 0.000952381 g/l Thiamine-HCl x 2 H2O 0.000952381 g/l Folic acid 0.00047619 g/l Pyridoxine hydrochloride 0.00047619 g/l Calcium pantothenate 0.00047619 g/l p-Aminobenzoic acid 0.00047619 g/l H3BO3 0.000285714 g/l CoCl2 x 6 H2O 0.000190476 g/l ZnSO4 x 7 H2O 9.52381e-05 g/l Biotin 4.7619e-05 g/l Na2MoO4 x 2 H2O 2.85714e-05 g/l MnCl2 x 4 H2O 2.85714e-05 g/l NiCl2 x 6 H2O 1.90476e-05 g/l Vitamin B12 9.52381e-06 g/l CuCl2 x 2 H2O 9.52381e-06 g/l Fe(III) citrate Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
22907positivegrowth50thermophilic
22907positivegrowth60thermophilic
3599positivegrowth55thermophilic
69480thermophilic90.251

Physiology and metabolism

oxygen tolerance

  • @ref: 3599
  • oxygen tolerance: anaerobe

nutrition type

@reftype
22907photoheterotroph
22907chemoheterotroph

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.717

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2290730089acetate+/-anaerobic growth with light
2290730089acetate-anaerobic growth in the dark
2290730089acetate-aerobic growth
2290735391aspartate+anaerobic growth with light
2290735391aspartate-anaerobic growth in the dark
2290735391aspartate-aerobic growth
2290717968butyrate+anaerobic growth with light
2290717968butyrate-anaerobic growth in the dark
2290717968butyrate-aerobic growth
22907casamino acids+anaerobic growth with light
22907casamino acids-anaerobic growth in the dark
22907casamino acids+aerobic growth
2290716947citrate+/-anaerobic growth with light
2290716947citrate-anaerobic growth in the dark
2290716947citrate-aerobic growth
2290716236ethanol+/-anaerobic growth with light
2290716236ethanol-anaerobic growth in the dark
2290716236ethanol-aerobic growth
2290728757fructose+/-anaerobic growth with light
2290728757fructose-anaerobic growth in the dark
2290728757fructose-aerobic growth
2290717234glucose+anaerobic growth with light
2290717234glucose+/-anaerobic growth in the dark
2290717234glucose-aerobic growth
2290729987glutamate+anaerobic growth with light
2290729987glutamate-anaerobic growth in the dark
2290729987glutamate-aerobic growth
2290717754glycerol+/-anaerobic growth with light
2290717754glycerol-anaerobic growth in the dark
2290717754glycerol-aerobic growth
2290717201glycylglycine-anaerobic growth with light
2290717201glycylglycine-anaerobic growth in the dark
2290717201glycylglycine-aerobic growth
2290717544hydrogencarbonate-anaerobic growth with light
2290717544hydrogencarbonate-anaerobic growth in the dark
2290717544hydrogencarbonate-aerobic growth
2290724996lactate+anaerobic growth with light
2290724996lactate-anaerobic growth in the dark
2290724996lactate-aerobic growth
2290725115malate+anaerobic growth with light
2290725115malate-anaerobic growth in the dark
2290725115malate-aerobic growth
2290729864mannitol+/-anaerobic growth with light
2290729864mannitol-anaerobic growth in the dark
2290729864mannitol-aerobic growth
2290717790methanol+/-anaerobic growth with light
2290717790methanol-anaerobic growth in the dark
2290715361pyruvate-anaerobic growth with light
2290715361pyruvate-anaerobic growth in the dark
2290715361pyruvate-aerobic growth
2290730031succinate+anaerobic growth with light
2290730031succinate-anaerobic growth in the dark
2290730031succinate-aerobic growth
22907yeast extract+anaerobic growth with light
22907yeast extract-anaerobic growth in the dark
22907yeast extract+aerobic growth
2290726948thiamine+required for growth
2290762501folate+required for growth

Isolation, sampling and environmental information

isolation

  • @ref: 3599
  • sample type: Meotobuchi hot spring
  • geographic location: Tochigi prefecture
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 3599
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Chloroflexus aggregans DSM 9485GCA_000021945completencbi326427
66792Chloroflexus aggregans DSM 9485326427.4completepatric326427
66792Chloroflexus aggregans DSM 9485643348527completeimg326427

GC content

  • @ref: 3599
  • GC-content: 56.7
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno88no
motileno83.123no
flagellatedno94.601no
gram-positiveno82.667no
anaerobicno73.698no
aerobicno77.867no
halophileno94.002no
spore-formingno82.611no
thermophileyes99.635no
glucose-utilyes82.196yes
glucose-fermentno82.792no

External links

@ref: 3599

culture collection no.: DSM 9485

straininfo link

  • @ref: 71971
  • straininfo: 48636

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7547286Chloroflexus aggregans sp. nov., a filamentous phototrophic bacterium which forms dense cell aggregates by active gliding movement.Hanada S, Hiraishi A, Shimada K, Matsuura KInt J Syst Bacteriol10.1099/00207713-45-4-6761995Bacteria/*classification/metabolism, Base Sequence, DNA, Bacterial/analysis, Molecular Sequence Data, *PhotosynthesisGenetics
Phylogeny28126046Chloroflexus islandicus sp. nov., a thermophilic filamentous anoxygenic phototrophic bacterium from a geyser.Gaisin VA, Kalashnikov AM, Grouzdev DS, Sukhacheva MV, Kuznetsov BB, Gorlenko VMInt J Syst Evol Microbiol10.1099/ijsem.0.0018202017Bacterial Proteins/chemistry, Bacterial Typing Techniques, Bacteriochlorophylls/chemistry, Base Composition, Carotenoids/chemistry, Chloroflexus/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Iceland, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water MicrobiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3599Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9485)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9485
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
22907SATOSHI HANADA, AKIRA HIRAISHI, KEIZO SHIMADA, KATSUMI MATSUURA10.1099/00207713-45-4-676Chloroflexus aggregans sp. nov., a Filamentous Phototrophic Bacterium Which Forms Dense Cell Aggregates by Active Gliding MovementIJSEM 45: 676-681 19957547286
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71971Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48636.1StrainInfo: A central database for resolving microbial strain identifiers