Strain identifier
BacDive ID: 2421
Type strain:
Species: Chloroflexus aggregans
Strain Designation: MD-66
Strain history: <- S. Hanada, MD-66
NCBI tax ID(s): 326427 (strain), 152260 (species)
General
@ref: 3599
BacDive-ID: 2421
DSM-Number: 9485
keywords: genome sequence, Bacteria, anaerobe, photoheterotroph, thermophilic
description: Chloroflexus aggregans MD-66 is an anaerobe, photoheterotroph, thermophilic bacterium that was isolated from Meotobuchi hot spring.
NCBI tax id
NCBI tax id | Matching level |
---|---|
152260 | species |
326427 | strain |
strain history
- @ref: 3599
- history: <- S. Hanada, MD-66
doi: 10.13145/bacdive2421.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/chloroflexota
- domain: Bacteria
- phylum: Chloroflexota
- class: Chloroflexia
- order: Chloroflexales
- family: Chloroflexaceae
- genus: Chloroflexus
- species: Chloroflexus aggregans
- full scientific name: Chloroflexus aggregans Hanada et al. 1995
@ref: 3599
domain: Bacteria
phylum: Chloroflexi
class: Chloroflexia
order: Chloroflexales, not validated
family: Chloroflexaceae
genus: Chloroflexus
species: Chloroflexus aggregans
full scientific name: Chloroflexus aggregans Hanada et al. 1995
strain designation: MD-66
type strain: yes
Morphology
cell morphology
@ref | cell length | gram stain | confidence |
---|---|---|---|
22907 | 1-1.5 µm | ||
69480 | positive | 94.131 |
Culture and growth conditions
culture medium
- @ref: 3599
- name: CHLOROFLEXUS AGGREGANS MEDIUM (DSMZ Medium 87a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/87a
- composition: Name: CHLOROFLEXUS AGGREGANS MEDIUM (DSMZ Medium 87a) Composition: Glycyl-glycine 0.952381 g/l Yeast extract 0.952381 g/l NaNO3 0.47619 g/l NaCl 0.0952381 g/l KNO3 0.0952381 g/l MgSO4 x 7 H2O 0.0952381 g/l Na2HPO4 x 2 H2O 0.0952381 g/l CaCl2 x 2 H2O 0.047619 g/l Na3-EDTA 0.00190476 g/l Nicotinic acid 0.000952381 g/l Thiamine-HCl x 2 H2O 0.000952381 g/l Folic acid 0.00047619 g/l Pyridoxine hydrochloride 0.00047619 g/l Calcium pantothenate 0.00047619 g/l p-Aminobenzoic acid 0.00047619 g/l H3BO3 0.000285714 g/l CoCl2 x 6 H2O 0.000190476 g/l ZnSO4 x 7 H2O 9.52381e-05 g/l Biotin 4.7619e-05 g/l Na2MoO4 x 2 H2O 2.85714e-05 g/l MnCl2 x 4 H2O 2.85714e-05 g/l NiCl2 x 6 H2O 1.90476e-05 g/l Vitamin B12 9.52381e-06 g/l CuCl2 x 2 H2O 9.52381e-06 g/l Fe(III) citrate Distilled water
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
22907 | positive | growth | 50 | thermophilic | |
22907 | positive | growth | 60 | thermophilic | |
3599 | positive | growth | 55 | thermophilic | |
69480 | thermophilic | 90.251 |
Physiology and metabolism
oxygen tolerance
- @ref: 3599
- oxygen tolerance: anaerobe
nutrition type
@ref | type |
---|---|
22907 | photoheterotroph |
22907 | chemoheterotroph |
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.717
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
22907 | 30089 | acetate | +/- | anaerobic growth with light |
22907 | 30089 | acetate | - | anaerobic growth in the dark |
22907 | 30089 | acetate | - | aerobic growth |
22907 | 35391 | aspartate | + | anaerobic growth with light |
22907 | 35391 | aspartate | - | anaerobic growth in the dark |
22907 | 35391 | aspartate | - | aerobic growth |
22907 | 17968 | butyrate | + | anaerobic growth with light |
22907 | 17968 | butyrate | - | anaerobic growth in the dark |
22907 | 17968 | butyrate | - | aerobic growth |
22907 | casamino acids | + | anaerobic growth with light | |
22907 | casamino acids | - | anaerobic growth in the dark | |
22907 | casamino acids | + | aerobic growth | |
22907 | 16947 | citrate | +/- | anaerobic growth with light |
22907 | 16947 | citrate | - | anaerobic growth in the dark |
22907 | 16947 | citrate | - | aerobic growth |
22907 | 16236 | ethanol | +/- | anaerobic growth with light |
22907 | 16236 | ethanol | - | anaerobic growth in the dark |
22907 | 16236 | ethanol | - | aerobic growth |
22907 | 28757 | fructose | +/- | anaerobic growth with light |
22907 | 28757 | fructose | - | anaerobic growth in the dark |
22907 | 28757 | fructose | - | aerobic growth |
22907 | 17234 | glucose | + | anaerobic growth with light |
22907 | 17234 | glucose | +/- | anaerobic growth in the dark |
22907 | 17234 | glucose | - | aerobic growth |
22907 | 29987 | glutamate | + | anaerobic growth with light |
22907 | 29987 | glutamate | - | anaerobic growth in the dark |
22907 | 29987 | glutamate | - | aerobic growth |
22907 | 17754 | glycerol | +/- | anaerobic growth with light |
22907 | 17754 | glycerol | - | anaerobic growth in the dark |
22907 | 17754 | glycerol | - | aerobic growth |
22907 | 17201 | glycylglycine | - | anaerobic growth with light |
22907 | 17201 | glycylglycine | - | anaerobic growth in the dark |
22907 | 17201 | glycylglycine | - | aerobic growth |
22907 | 17544 | hydrogencarbonate | - | anaerobic growth with light |
22907 | 17544 | hydrogencarbonate | - | anaerobic growth in the dark |
22907 | 17544 | hydrogencarbonate | - | aerobic growth |
22907 | 24996 | lactate | + | anaerobic growth with light |
22907 | 24996 | lactate | - | anaerobic growth in the dark |
22907 | 24996 | lactate | - | aerobic growth |
22907 | 25115 | malate | + | anaerobic growth with light |
22907 | 25115 | malate | - | anaerobic growth in the dark |
22907 | 25115 | malate | - | aerobic growth |
22907 | 29864 | mannitol | +/- | anaerobic growth with light |
22907 | 29864 | mannitol | - | anaerobic growth in the dark |
22907 | 29864 | mannitol | - | aerobic growth |
22907 | 17790 | methanol | +/- | anaerobic growth with light |
22907 | 17790 | methanol | - | anaerobic growth in the dark |
22907 | 15361 | pyruvate | - | anaerobic growth with light |
22907 | 15361 | pyruvate | - | anaerobic growth in the dark |
22907 | 15361 | pyruvate | - | aerobic growth |
22907 | 30031 | succinate | + | anaerobic growth with light |
22907 | 30031 | succinate | - | anaerobic growth in the dark |
22907 | 30031 | succinate | - | aerobic growth |
22907 | yeast extract | + | anaerobic growth with light | |
22907 | yeast extract | - | anaerobic growth in the dark | |
22907 | yeast extract | + | aerobic growth | |
22907 | 26948 | thiamine | + | required for growth |
22907 | 62501 | folate | + | required for growth |
Isolation, sampling and environmental information
isolation
- @ref: 3599
- sample type: Meotobuchi hot spring
- geographic location: Tochigi prefecture
- country: Japan
- origin.country: JPN
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 3599
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Chloroflexus aggregans DSM 9485 | GCA_000021945 | complete | ncbi | 326427 |
66792 | Chloroflexus aggregans DSM 9485 | 326427.4 | complete | patric | 326427 |
66792 | Chloroflexus aggregans DSM 9485 | 643348527 | complete | img | 326427 |
GC content
- @ref: 3599
- GC-content: 56.7
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 88 | no |
motile | no | 83.123 | no |
flagellated | no | 94.601 | no |
gram-positive | no | 82.667 | no |
anaerobic | no | 73.698 | no |
aerobic | no | 77.867 | no |
halophile | no | 94.002 | no |
spore-forming | no | 82.611 | no |
thermophile | yes | 99.635 | no |
glucose-util | yes | 82.196 | yes |
glucose-ferment | no | 82.792 | no |
External links
@ref: 3599
culture collection no.: DSM 9485
straininfo link
- @ref: 71971
- straininfo: 48636
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 7547286 | Chloroflexus aggregans sp. nov., a filamentous phototrophic bacterium which forms dense cell aggregates by active gliding movement. | Hanada S, Hiraishi A, Shimada K, Matsuura K | Int J Syst Bacteriol | 10.1099/00207713-45-4-676 | 1995 | Bacteria/*classification/metabolism, Base Sequence, DNA, Bacterial/analysis, Molecular Sequence Data, *Photosynthesis | Genetics |
Phylogeny | 28126046 | Chloroflexus islandicus sp. nov., a thermophilic filamentous anoxygenic phototrophic bacterium from a geyser. | Gaisin VA, Kalashnikov AM, Grouzdev DS, Sukhacheva MV, Kuznetsov BB, Gorlenko VM | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001820 | 2017 | Bacterial Proteins/chemistry, Bacterial Typing Techniques, Bacteriochlorophylls/chemistry, Base Composition, Carotenoids/chemistry, Chloroflexus/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Iceland, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water Microbiology | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
3599 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9485) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-9485 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
22907 | SATOSHI HANADA, AKIRA HIRAISHI, KEIZO SHIMADA, KATSUMI MATSUURA | 10.1099/00207713-45-4-676 | Chloroflexus aggregans sp. nov., a Filamentous Phototrophic Bacterium Which Forms Dense Cell Aggregates by Active Gliding Movement | IJSEM 45: 676-681 1995 | 7547286 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
71971 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48636.1 | StrainInfo: A central database for resolving microbial strain identifiers |