Strain identifier
BacDive ID: 24172
Type strain: 
Species: Methylobacterium thuringiense
Strain Designation: T19+3-5, T5
Strain history: <- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; T19+3-5 <- S.A. Wellner
NCBI tax ID(s): 1003091 (species)
General
@ref: 20647
BacDive-ID: 24172
DSM-Number: 23631
keywords: 16S sequence, Bacteria, mesophilic
description: Methylobacterium thuringiense T19+3-5 is a mesophilic bacterium that was isolated from phyllosphere of Trifolium repens.
NCBI tax id
- NCBI tax id: 1003091
- Matching level: species
strain history
- @ref: 20647
- history: <- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; T19+3-5 <- S.A. Wellner
doi: 10.13145/bacdive24172.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Methylobacteriaceae
- genus: Methylobacterium
- species: Methylobacterium thuringiense
- full scientific name: Methylobacterium thuringiense Wellner et al. 2013
@ref: 20647
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Methylobacteriaceae
genus: Methylobacterium
species: Methylobacterium thuringiense
full scientific name: Methylobacterium thuringiense Wellner et al. 2013
strain designation: T19+3-5, T5
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 20647 | METHYLOBACTERIUM MEDIUM (DSMZ Medium 125) | yes | https://mediadive.dsmz.de/medium/125 | Name: METHYLOBACTERIUM MEDIUM (DSMZ Medium 125) Composition: Agar 12.0 g/l KNO3 1.0 g/l Na2HPO4 0.23 g/l MgSO4 x 7 H2O 0.2 g/l NaH2PO4 0.07 g/l CaCl2 x 2 H2O 0.02 g/l FeSO4 x 7 H2O 0.001 g/l ZnSO4 x 7 H2O 7e-05 g/l H3BO3 1e-05 g/l MnSO4 x 5 H2O 1e-05 g/l MoO3 1e-05 g/l CuSO4 x 5 H2O 5e-06 g/l Distilled water |
| 20647 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1; with strain-specific modifications) Composition: Agar 15.0 g/l Methanol 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 20647 | positive | growth | 28 | mesophilic |
| 20647 | positive | growth | 20-28 |
Isolation, sampling and environmental information
isolation
- @ref: 20647
- sample type: phyllosphere of Trifolium repens
- host species: Trifolium repens
- geographic location: Thüringen, exploratory Hainich-Dün
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Leaf (Phyllosphere) |
Safety information
risk assessment
- @ref: 20647
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20647
- description: Methylobacterium sp. T5 partial 16S rRNA gene, strain DSM 23631, isolate T5
- accession: FR847846
- length: 1350
- database: ena
- NCBI tax ID: 1003090
External links
@ref: 20647
culture collection no.: DSM 23631, CCM 7790
straininfo link
- @ref: 88127
- straininfo: 400233
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 20647 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23631 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23631) | |
| 88127 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID400233.1 |