Strain identifier

BacDive ID: 2417

Type strain: Yes

Species: Chloroflexus aurantiacus

Strain Designation: J-10-fl

Strain history: <- T.D. Brock <- R.W. Castenholz, J-10-fl

NCBI tax ID(s): 324602 (strain), 1108 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 337

BacDive-ID: 2417

DSM-Number: 635

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-positive

description: Chloroflexus aurantiacus J-10-fl is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from hakone Hot Spring area.

NCBI tax id

NCBI tax idMatching level
1108species
324602strain

strain history

  • @ref: 337
  • history: <- T.D. Brock <- R.W. Castenholz, J-10-fl

doi: 10.13145/bacdive2417.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/chloroflexota
  • domain: Bacteria
  • phylum: Chloroflexota
  • class: Chloroflexia
  • order: Chloroflexales
  • family: Chloroflexaceae
  • genus: Chloroflexus
  • species: Chloroflexus aurantiacus
  • full scientific name: Chloroflexus aurantiacus Pierson and Castenholz 1974 (Approved Lists 1980)

@ref: 337

domain: Bacteria

phylum: Chloroflexi

class: Chloroflexia

order: Chloroflexales, not validated

family: Chloroflexaceae

genus: Chloroflexus

species: Chloroflexus aurantiacus

full scientific name: Chloroflexus aurantiacus Pierson and Castenholz 1974 emend. Nouioui et al. 2018

strain designation: J-10-fl

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 96.482

Culture and growth conditions

culture medium

  • @ref: 337
  • name: CHLOROFLEXUS MEDIUM (modified) (DSMZ Medium 87)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/87
  • composition: Name: CHLOROFLEXUS MEDIUM (modified) (DSMZ Medium 87) Composition: Yeast extract 0.94697 g/l Glycyl-glycine 0.94697 g/l NaNO3 0.473485 g/l MgSO4 x 7 H2O 0.094697 g/l KNO3 0.094697 g/l NaCl 0.094697 g/l Na2HPO4 x 2 H2O 0.094697 g/l CaCl2 x 2 H2O 0.0473485 g/l H3BO3 0.000284091 g/l CoCl2 x 6 H2O 0.000189394 g/l ZnSO4 x 7 H2O 9.4697e-05 g/l MnCl2 x 4 H2O 2.84091e-05 g/l Na2MoO4 x 2 H2O 2.84091e-05 g/l NiCl2 x 6 H2O 1.89394e-05 g/l CuCl2 x 2 H2O 9.4697e-06 g/l Fe(III) citrate Distilled water

culture temp

  • @ref: 337
  • growth: positive
  • type: growth
  • temperature: 49
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 337
  • oxygen tolerance: anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.84

Isolation, sampling and environmental information

isolation

  • @ref: 337
  • sample type: hakone Hot Spring area
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 337
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Chloroflexus aurantiacus gene for 16S ribosomal RNA, partial sequence
  • accession: D38365
  • length: 1401
  • database: ena
  • NCBI tax ID: 324602

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Chloroflexus aurantiacus J-10-flGCA_000018865completencbi324602
66792Chloroflexus aurantiacus J-10-fl324602.8completepatric324602
66792Chloroflexus aurantiacus J-10-fl641228485completeimg324602

GC content

  • @ref: 337
  • GC-content: 54.9

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno80no
motileno83.536no
flagellatedno93.862no
gram-positiveno75.885no
anaerobicno83.531no
aerobicno72.175yes
halophileno92.808no
spore-formingno83.483no
thermophileyes99.155yes
glucose-utilyes79.289no
glucose-fermentno84.185no

External links

@ref: 337

culture collection no.: DSM 635, ATCC 29366

straininfo link

  • @ref: 71967
  • straininfo: 46690

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism2350541Red shift of absorption maxima in chlorobiineae through enzymic methylation of their antenna bacteriochlorophylls.Bobe FW, Pfennig N, Swanson KL, Smith KMBiochemistry10.1021/bi00470a0121990Bacteria/*metabolism, Bacteriochlorophylls/*metabolism, Carbon Isotopes, Chlorophyll/*analogs & derivatives, Chromatography, High Pressure Liquid, Magnetic Resonance Spectroscopy, Methionine/*metabolism, Methylation, Photosynthesis, Spectrophotometry
Phylogeny7275928Isolation and development of chlorosomes in the green bacterium Chloroflexus aurantiacus.Sprague SG, Staehelin LA, DiBartolomeis MJ, Fuller RCJ Bacteriol10.1128/jb.147.3.1021-1031.19811981Bacteriochlorophylls/biosynthesis, Cell Fractionation, Cell Membrane/ultrastructure, Freeze Fracturing, Light, Microscopy, Electron, Organoids/ultrastructure, Oxygen/physiology, *Photosynthesis, Rhodospirillales/metabolism/*ultrastructureMetabolism
Metabolism7275929Semiaerobic induction of bacteriochlorophyll synthesis in the green bacterium Chloroflexus aurantiacus.Sprague SG, Staehelin LA, Fuller RCJ Bacteriol10.1128/jb.147.3.1032-1039.19811981Aerobiosis, Bacteriochlorophylls/*biosynthesis, Chlorophyll/*analogs & derivatives, Freeze Fracturing, Light, Microscopy, Electron, Oxygen/*physiology, Rhodospirillales/growth & development/*metabolism/ultrastructure
Enzymology7764368Purification and characterization of a thermostable neutral metalloprotease I from Chloroflexus aurantiacus J-10-fl.Watanabe A, Kamio Y, Kimura W, Izaki KBiosci Biotechnol Biochem10.1271/bbb.57.21601993Amino Acid Sequence, Bacteria/*enzymology, Calcium Chloride/pharmacology, Chromatography, High Pressure Liquid, Culture Media, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Insulin/metabolism, Isoelectric Point, Metalloendopeptidases/chemistry/*isolation & purification/*metabolism, Molecular Sequence Data, Molecular Weight, Pancreatic Elastase/metabolism, TemperatureMetabolism
Enzymology20465238Kinetic and structural characterization of a heterohexamer 4-oxalocrotonate tautomerase from Chloroflexus aurantiacus J-10-fl: implications for functional and structural diversity in the tautomerase superfamily .Burks EA, Fleming CD, Mesecar AD, Whitman CP, Pegan SDBiochemistry10.1021/bi100502z2010Amino Acid Sequence, Bacterial Proteins/*chemistry/genetics, Catalytic Domain, *Chloroflexus, Crystallography, X-Ray, Enzyme Stability, Isoenzymes/chemistry/genetics, Isomerases/*chemistry/genetics, Kinetics, Models, Molecular, Molecular Sequence Data, Mutation, Protein Conformation, Protein Multimerization, Protein Subunits/chemistry, Recombinant Proteins/chemistry, Sequence AlignmentGenetics
Metabolism21723339Production of 3-hydroxypropionic acid via malonyl-CoA pathway using recombinant Escherichia coli strains.Rathnasingh C, Raj SM, Lee Y, Catherine C, Ashok S, Park SJ Biotechnol10.1016/j.jbiotec.2011.06.0082011Acetyl-CoA Carboxylase/metabolism, Aerobiosis, Anaerobiosis, Escherichia coli/cytology/enzymology/*genetics/growth & development, Gene Deletion, Glucose/metabolism, Lactic Acid/*analogs & derivatives/biosynthesis, Malonyl Coenzyme A/*metabolism, *Metabolic Networks and Pathways, NADP Transhydrogenases/metabolism, Oxidoreductases/metabolism, Plasmids/genetics, Recombination, Genetic/*genetics, Temperature, Time FactorsEnzymology
Metabolism22249883Comparison of Chloroflexus aurantiacus strain J-10-fl proteomes of cells grown chemoheterotrophically and photoheterotrophically.Cao L, Bryant DA, Schepmoes AA, Vogl K, Smith RD, Lipton MS, Callister SJPhotosynth Res10.1007/s11120-011-9711-82012Bacterial Proteins/metabolism, Biosynthetic Pathways/radiation effects, Carbon Dioxide/metabolism, Chloroflexus/cytology/*growth & development/*metabolism/radiation effects, Heterotrophic Processes/*radiation effects, *Light, Peptides/metabolism, Proteome/*metabolism, Proteomics, Reproducibility of ResultsProteome
Genetics25526209Evaluating the annotation of protein-coding genes in bacterial genomes: Chloroflexus aurantiacus strain J-10-fl and Natrinema sp J7-2 as case studies.Zhang HX, Li SJ, Zhou HQGenet Mol Res10.4238/2014.December.19.102014Archaeal Proteins/genetics, Bacterial Proteins/genetics, Chloroflexus/classification/*genetics, Genome Size, Genome, Archaeal, Genome, Bacterial, Halobacteriaceae/classification/*genetics, Molecular Sequence Annotation/*methods, Sequence Analysis, DNAPhylogeny
Metabolism26669591Live Cell Discovery of Microbial Vitamin Transport and Enzyme-Cofactor Interactions.Anderson LN, Koech PK, Plymale AE, Landorf EV, Konopka A, Collart FR, Lipton MS, Romine MF, Wright ATACS Chem Biol10.1021/acschembio.5b009182015Bacterial Proteins/*metabolism, Biological Transport, Chloroflexus/cytology/*metabolism, Molecular Probe Techniques, Optical Imaging, Proteome/metabolism, Staining and Labeling, Vitamin B Complex/*metabolismEnzymology
26732979Glycine Cleavage Powers Photoheterotrophic Growth of Chloroflexus aurantiacus in the Absence of H 2.He L, Wang Y, You L, Khin Y, Tang JK, Tang YJFront Microbiol10.3389/fmicb.2015.014672015
Metabolism32418929Aerobic Production of Bacteriochlorophylls in the Filamentous Anoxygenic Photosynthetic Bacterium, Chloroflexus aurantiacus in the Light.Izaki K, Haruta SMicrobes Environ10.1264/jsme2.ME200152020Adenosine Triphosphate/biosynthesis, Bacterial Proteins/genetics, Bacteriochlorophylls/*biosynthesis/genetics, Chloroflexus/*metabolism, Gene Expression Regulation, Bacterial, *Light, Oxygen/*metabolism, Photosynthesis, Photosynthetic Reaction Center Complex Proteins/geneticsBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
337Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 635)https://www.dsmz.de/collection/catalogue/details/culture/DSM-635
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71967Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46690.1StrainInfo: A central database for resolving microbial strain identifiers