Strain identifier
BacDive ID: 2417
Type strain:
Species: Chloroflexus aurantiacus
Strain Designation: J-10-fl
Strain history: <- T.D. Brock <- R.W. Castenholz, J-10-fl
NCBI tax ID(s): 324602 (strain), 1108 (species)
General
@ref: 337
BacDive-ID: 2417
DSM-Number: 635
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-positive
description: Chloroflexus aurantiacus J-10-fl is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from hakone Hot Spring area.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1108 | species |
324602 | strain |
strain history
- @ref: 337
- history: <- T.D. Brock <- R.W. Castenholz, J-10-fl
doi: 10.13145/bacdive2417.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/chloroflexota
- domain: Bacteria
- phylum: Chloroflexota
- class: Chloroflexia
- order: Chloroflexales
- family: Chloroflexaceae
- genus: Chloroflexus
- species: Chloroflexus aurantiacus
- full scientific name: Chloroflexus aurantiacus Pierson and Castenholz 1974 (Approved Lists 1980)
@ref: 337
domain: Bacteria
phylum: Chloroflexi
class: Chloroflexia
order: Chloroflexales, not validated
family: Chloroflexaceae
genus: Chloroflexus
species: Chloroflexus aurantiacus
full scientific name: Chloroflexus aurantiacus Pierson and Castenholz 1974 emend. Nouioui et al. 2018
strain designation: J-10-fl
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 96.482
Culture and growth conditions
culture medium
- @ref: 337
- name: CHLOROFLEXUS MEDIUM (modified) (DSMZ Medium 87)
- growth: yes
- link: https://mediadive.dsmz.de/medium/87
- composition: Name: CHLOROFLEXUS MEDIUM (modified) (DSMZ Medium 87) Composition: Yeast extract 0.94697 g/l Glycyl-glycine 0.94697 g/l NaNO3 0.473485 g/l MgSO4 x 7 H2O 0.094697 g/l KNO3 0.094697 g/l NaCl 0.094697 g/l Na2HPO4 x 2 H2O 0.094697 g/l CaCl2 x 2 H2O 0.0473485 g/l H3BO3 0.000284091 g/l CoCl2 x 6 H2O 0.000189394 g/l ZnSO4 x 7 H2O 9.4697e-05 g/l MnCl2 x 4 H2O 2.84091e-05 g/l Na2MoO4 x 2 H2O 2.84091e-05 g/l NiCl2 x 6 H2O 1.89394e-05 g/l CuCl2 x 2 H2O 9.4697e-06 g/l Fe(III) citrate Distilled water
culture temp
- @ref: 337
- growth: positive
- type: growth
- temperature: 49
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 337
- oxygen tolerance: anaerobe
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.84
Isolation, sampling and environmental information
isolation
- @ref: 337
- sample type: hakone Hot Spring area
- country: Japan
- origin.country: JPN
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 337
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20218
- description: Chloroflexus aurantiacus gene for 16S ribosomal RNA, partial sequence
- accession: D38365
- length: 1401
- database: ena
- NCBI tax ID: 324602
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Chloroflexus aurantiacus J-10-fl | GCA_000018865 | complete | ncbi | 324602 |
66792 | Chloroflexus aurantiacus J-10-fl | 324602.8 | complete | patric | 324602 |
66792 | Chloroflexus aurantiacus J-10-fl | 641228485 | complete | img | 324602 |
GC content
- @ref: 337
- GC-content: 54.9
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 80 | no |
motile | no | 83.536 | no |
flagellated | no | 93.862 | no |
gram-positive | no | 75.885 | no |
anaerobic | no | 83.531 | no |
aerobic | no | 72.175 | yes |
halophile | no | 92.808 | no |
spore-forming | no | 83.483 | no |
thermophile | yes | 99.155 | yes |
glucose-util | yes | 79.289 | no |
glucose-ferment | no | 84.185 | no |
External links
@ref: 337
culture collection no.: DSM 635, ATCC 29366
straininfo link
- @ref: 71967
- straininfo: 46690
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Metabolism | 2350541 | Red shift of absorption maxima in chlorobiineae through enzymic methylation of their antenna bacteriochlorophylls. | Bobe FW, Pfennig N, Swanson KL, Smith KM | Biochemistry | 10.1021/bi00470a012 | 1990 | Bacteria/*metabolism, Bacteriochlorophylls/*metabolism, Carbon Isotopes, Chlorophyll/*analogs & derivatives, Chromatography, High Pressure Liquid, Magnetic Resonance Spectroscopy, Methionine/*metabolism, Methylation, Photosynthesis, Spectrophotometry | |
Phylogeny | 7275928 | Isolation and development of chlorosomes in the green bacterium Chloroflexus aurantiacus. | Sprague SG, Staehelin LA, DiBartolomeis MJ, Fuller RC | J Bacteriol | 10.1128/jb.147.3.1021-1031.1981 | 1981 | Bacteriochlorophylls/biosynthesis, Cell Fractionation, Cell Membrane/ultrastructure, Freeze Fracturing, Light, Microscopy, Electron, Organoids/ultrastructure, Oxygen/physiology, *Photosynthesis, Rhodospirillales/metabolism/*ultrastructure | Metabolism |
Metabolism | 7275929 | Semiaerobic induction of bacteriochlorophyll synthesis in the green bacterium Chloroflexus aurantiacus. | Sprague SG, Staehelin LA, Fuller RC | J Bacteriol | 10.1128/jb.147.3.1032-1039.1981 | 1981 | Aerobiosis, Bacteriochlorophylls/*biosynthesis, Chlorophyll/*analogs & derivatives, Freeze Fracturing, Light, Microscopy, Electron, Oxygen/*physiology, Rhodospirillales/growth & development/*metabolism/ultrastructure | |
Enzymology | 7764368 | Purification and characterization of a thermostable neutral metalloprotease I from Chloroflexus aurantiacus J-10-fl. | Watanabe A, Kamio Y, Kimura W, Izaki K | Biosci Biotechnol Biochem | 10.1271/bbb.57.2160 | 1993 | Amino Acid Sequence, Bacteria/*enzymology, Calcium Chloride/pharmacology, Chromatography, High Pressure Liquid, Culture Media, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Insulin/metabolism, Isoelectric Point, Metalloendopeptidases/chemistry/*isolation & purification/*metabolism, Molecular Sequence Data, Molecular Weight, Pancreatic Elastase/metabolism, Temperature | Metabolism |
Enzymology | 20465238 | Kinetic and structural characterization of a heterohexamer 4-oxalocrotonate tautomerase from Chloroflexus aurantiacus J-10-fl: implications for functional and structural diversity in the tautomerase superfamily . | Burks EA, Fleming CD, Mesecar AD, Whitman CP, Pegan SD | Biochemistry | 10.1021/bi100502z | 2010 | Amino Acid Sequence, Bacterial Proteins/*chemistry/genetics, Catalytic Domain, *Chloroflexus, Crystallography, X-Ray, Enzyme Stability, Isoenzymes/chemistry/genetics, Isomerases/*chemistry/genetics, Kinetics, Models, Molecular, Molecular Sequence Data, Mutation, Protein Conformation, Protein Multimerization, Protein Subunits/chemistry, Recombinant Proteins/chemistry, Sequence Alignment | Genetics |
Metabolism | 21723339 | Production of 3-hydroxypropionic acid via malonyl-CoA pathway using recombinant Escherichia coli strains. | Rathnasingh C, Raj SM, Lee Y, Catherine C, Ashok S, Park S | J Biotechnol | 10.1016/j.jbiotec.2011.06.008 | 2011 | Acetyl-CoA Carboxylase/metabolism, Aerobiosis, Anaerobiosis, Escherichia coli/cytology/enzymology/*genetics/growth & development, Gene Deletion, Glucose/metabolism, Lactic Acid/*analogs & derivatives/biosynthesis, Malonyl Coenzyme A/*metabolism, *Metabolic Networks and Pathways, NADP Transhydrogenases/metabolism, Oxidoreductases/metabolism, Plasmids/genetics, Recombination, Genetic/*genetics, Temperature, Time Factors | Enzymology |
Metabolism | 22249883 | Comparison of Chloroflexus aurantiacus strain J-10-fl proteomes of cells grown chemoheterotrophically and photoheterotrophically. | Cao L, Bryant DA, Schepmoes AA, Vogl K, Smith RD, Lipton MS, Callister SJ | Photosynth Res | 10.1007/s11120-011-9711-8 | 2012 | Bacterial Proteins/metabolism, Biosynthetic Pathways/radiation effects, Carbon Dioxide/metabolism, Chloroflexus/cytology/*growth & development/*metabolism/radiation effects, Heterotrophic Processes/*radiation effects, *Light, Peptides/metabolism, Proteome/*metabolism, Proteomics, Reproducibility of Results | Proteome |
Genetics | 25526209 | Evaluating the annotation of protein-coding genes in bacterial genomes: Chloroflexus aurantiacus strain J-10-fl and Natrinema sp J7-2 as case studies. | Zhang HX, Li SJ, Zhou HQ | Genet Mol Res | 10.4238/2014.December.19.10 | 2014 | Archaeal Proteins/genetics, Bacterial Proteins/genetics, Chloroflexus/classification/*genetics, Genome Size, Genome, Archaeal, Genome, Bacterial, Halobacteriaceae/classification/*genetics, Molecular Sequence Annotation/*methods, Sequence Analysis, DNA | Phylogeny |
Metabolism | 26669591 | Live Cell Discovery of Microbial Vitamin Transport and Enzyme-Cofactor Interactions. | Anderson LN, Koech PK, Plymale AE, Landorf EV, Konopka A, Collart FR, Lipton MS, Romine MF, Wright AT | ACS Chem Biol | 10.1021/acschembio.5b00918 | 2015 | Bacterial Proteins/*metabolism, Biological Transport, Chloroflexus/cytology/*metabolism, Molecular Probe Techniques, Optical Imaging, Proteome/metabolism, Staining and Labeling, Vitamin B Complex/*metabolism | Enzymology |
26732979 | Glycine Cleavage Powers Photoheterotrophic Growth of Chloroflexus aurantiacus in the Absence of H 2. | He L, Wang Y, You L, Khin Y, Tang JK, Tang YJ | Front Microbiol | 10.3389/fmicb.2015.01467 | 2015 | |||
Metabolism | 32418929 | Aerobic Production of Bacteriochlorophylls in the Filamentous Anoxygenic Photosynthetic Bacterium, Chloroflexus aurantiacus in the Light. | Izaki K, Haruta S | Microbes Environ | 10.1264/jsme2.ME20015 | 2020 | Adenosine Triphosphate/biosynthesis, Bacterial Proteins/genetics, Bacteriochlorophylls/*biosynthesis/genetics, Chloroflexus/*metabolism, Gene Expression Regulation, Bacterial, *Light, Oxygen/*metabolism, Photosynthesis, Photosynthetic Reaction Center Complex Proteins/genetics | Biotechnology |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
337 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 635) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-635 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
71967 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID46690.1 | StrainInfo: A central database for resolving microbial strain identifiers |