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Strain identifier

BacDive ID: 24166

Type strain: No

Species: Methanosarcina mazei

Strain Designation: O1M9701

Strain history: <- M.-C. Lai; O1M9701

NCBI tax ID(s): 2209 (species)

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General

@ref: 21016

BacDive-ID: 24166

DSM-Number: 27785

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanosarcina mazei O1M9701 is an anaerobe, mesophilic archaeon that was isolated from sediment of estuarine environment.

NCBI tax id

  • NCBI tax id: 2209
  • Matching level: species

strain history: <- M.-C. Lai; O1M9701

doi: 10.13145/bacdive24166.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/euryarchaeota
  • domain: Archaea
  • phylum: Euryarchaeota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanosarcina
  • species: Methanosarcina mazei
  • full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984
  • synonyms

    @refsynonym
    20215Methanosarcina frisia
    20215Methanococcus frisius
    20215Methanosarcina mazeii
    20215Methanococcus mazei

@ref: 21016

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina mazei

full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984

strain designation: O1M9701

type strain: no

Morphology

colony morphology

  • @ref: 21016
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 21016
  • name: METHANOSARCINA MB MEDIUM (1 G/L NACL) (DSMZ Medium 924b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/924b
  • composition: Name: METHANOSARCINA MB MEDIUM (1 g/l NaCl) (DSMZ Medium 924b; with strain-specific modifications) Composition: NaHCO3 3.95648 g/l Methanol 3.13353 g/l Yeast extract 1.97824 g/l Trypticase peptone 1.97824 g/l MgCl2 x 6 H2O 0.98912 g/l NH4Cl 0.98912 g/l KCl 0.49456 g/l K2HPO4 0.395648 g/l Na2S x 9 H2O 0.24728 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l H3BO3 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

  • @ref: 21016
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 21016
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 21016
  • sample type: sediment of estuarine environment
  • geographic location: Eriln Shi
  • country: Taiwan
  • origin.country: TWN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Estuary
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_187097.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_68776;97_89418;98_121696;99_187097&stattab=map
  • Last taxonomy: Methanosarcina
  • 16S sequence: AF411469
  • Sequence Identity:
  • Total samples: 7202
  • soil counts: 2215
  • aquatic counts: 3372
  • animal counts: 684
  • plant counts: 931

Safety information

risk assessment

  • @ref: 21016
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21016
  • description: Methanosarcina mazei strain O1M9701 16S ribosomal RNA gene, partial sequence
  • accession: AF411469
  • length: 1271
  • database: ena
  • NCBI tax ID: 2209

External links

@ref: 21016

culture collection no.: DSM 27785, OCM 748

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21016Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-27785Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27785)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/