General

@ref: 21016

BacDive-ID: 24166

DSM-Number: 27785

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanosarcina mazei O1M9701 is an anaerobe, mesophilic archaeon that was isolated from sediment of estuarine environment.

NCBI tax id

• NCBI tax id: 2209
• Matching level: species

strain history: <- M.-C. Lai; O1M9701

doi: 10.13145/bacdive24166.20221219.7.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/euryarchaeota
• domain: Archaea
• phylum: Euryarchaeota
• class: Methanomicrobia
• order: Methanosarcinales
• family: Methanosarcinaceae
• genus: Methanosarcina
• species: Methanosarcina mazei
• full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

• synonyms

  @ref	synonym
  20215	Methanosarcina frisia
  20215	Methanococcus frisius
  20215	Methanosarcina mazeii
  20215	Methanococcus mazei

@ref: 21016

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina mazei

full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984

strain designation: O1M9701

type strain: no

Morphology

colony morphology

• @ref: 21016
• incubation period: 2-3 days

Culture and growth conditions

culture medium

• @ref: 21016
• name: METHANOSARCINA MB MEDIUM (1 G/L NACL) (DSMZ Medium 924b)
• growth: yes
• link: https://mediadive.dsmz.de/medium/924b
• composition: Name: METHANOSARCINA MB MEDIUM (1 g/l NaCl) (DSMZ Medium 924b; with strain-specific modifications) Composition: NaHCO3 3.95648 g/l Methanol 3.13353 g/l Yeast extract 1.97824 g/l Trypticase peptone 1.97824 g/l MgCl2 x 6 H2O 0.98912 g/l NH4Cl 0.98912 g/l KCl 0.49456 g/l K2HPO4 0.395648 g/l Na2S x 9 H2O 0.24728 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l H3BO3 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

• @ref: 21016
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 21016
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 21016
• sample type: sediment of estuarine environment
• geographic location: Eriln Shi
• country: Taiwan
• origin.country: TWN
• continent: Asia

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_187097.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_68776;97_89418;98_121696;99_187097&stattab=map
• Last taxonomy: Methanosarcina
• 16S sequence: AF411469
• Sequence Identity:
• Total samples: 7202
• soil counts: 2215
• aquatic counts: 3372
• animal counts: 684
• plant counts: 931

Safety information

risk assessment

• @ref: 21016
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 21016
• description: Methanosarcina mazei strain O1M9701 16S ribosomal RNA gene, partial sequence
• accession: AF411469
• length: 1271
• database: ena
• NCBI tax ID: 2209

External links

@ref: 21016

culture collection no.: DSM 27785, OCM 748

Reference