Strain identifier
BacDive ID: 24164
Type strain: 
Species: Methanolobus chelungpuianus
Strain Designation: St545Mb
Strain history: M.-C. Lai St545Mb.
NCBI tax ID(s): 502115 (species)
version 8.1 (current version)
General
@ref: 21019
BacDive-ID: 24164
DSM-Number: 19953
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanolobus chelungpuianus St545Mb is an anaerobe, mesophilic archaeon that was isolated from Sandstone sample at a depth of 545 m.
NCBI tax id
- NCBI tax id: 502115
- Matching level: species
strain history
| @ref | history |
|---|---|
| 21019 | <- M.-C. Lai, Dept. Life Sci., Natl. Chung Hsing Univ., Taichung, Taiwan; St545Mb <- S.-Y. Wu and M.-C. Lai {2007} |
| 67770 | M.-C. Lai St545Mb. |
doi: 10.13145/bacdive24164.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanolobus
- species: Methanolobus chelungpuianus
- full scientific name: Methanolobus chelungpuianus Wu and Lai 2015
@ref: 21019
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanolobus
species: Methanolobus chelungpuianus
full scientific name: Methanolobus chelungpuianus Wu and Lai 2015
strain designation: St545Mb
type strain: yes
Culture and growth conditions
culture medium
- @ref: 21019
- name: METHANOFOLLIS MB MEDIUM (5 G/L NACL) (DSMZ Medium 924a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/924a
- composition: Name: METHANOFOLLIS MB MEDIUM (5 g/l NaCl) (DSMZ Medium 924a; with strain-specific modifications) Composition: NaHCO3 3.95648 g/l Yeast extract 1.97824 g/l Trypticase peptone 1.97824 g/l Methanol 1.17507 g/l MgCl2 x 6 H2O 0.98912 g/l NH4Cl 0.98912 g/l KCl 0.49456 g/l K2HPO4 0.395648 g/l Na2S x 9 H2O 0.24728 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 21019 | positive | growth | 37 | mesophilic |
| 67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 21019
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 21019 | Sandstone sample at a depth of 545 m | Taiwan, Taichung, Chelungpu fault | China | CHN | Asia |
| 67770 | Deep subsurface in sandstone in Chelungpu fault near Dai-Keng | Taiwan | TWN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Geologic
taxonmaps
- @ref: 69479
- File name: preview.99_189600.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_69981;97_90888;98_123509;99_189600&stattab=map
- Last taxonomy: Methanolobus chelungpuianus subclade
- 16S sequence: EU293796
- Sequence Identity:
- Total samples: 5
- aquatic counts: 5
Safety information
risk assessment
- @ref: 21019
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21019
- description: Methanolobus chelungpuianus strain St545Mb 16S ribosomal RNA gene, partial sequence
- accession: EU293796
- length: 1309
- database: ena
- NCBI tax ID: 502115
Genome sequences
- @ref: 66792
- description: Methanolobus chelungpuianus St545Mb
- accession: GCA_024500045
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 502115
GC content
- @ref: 21019
- GC-content: 48.3
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| flagellated | no | 96.904 | no |
| gram-positive | no | 96.69 | no |
| anaerobic | yes | 97.918 | no |
| aerobic | no | 94.866 | no |
| halophile | no | 79.093 | no |
| spore-forming | no | 95.461 | no |
| motile | no | 86.722 | no |
| glucose-ferment | no | 64.653 | no |
| thermophile | no | 91.869 | yes |
| glucose-util | yes | 70.261 | no |
External links
@ref: 21019
culture collection no.: DSM 19953, BCRC AR10030, JCM 15159
straininfo link
- @ref: 88120
- straininfo: 400193
literature
- topic: Phylogeny
- Pubmed-ID: 21148697
- title: Methanogenic archaea isolated from Taiwan's Chelungpu fault.
- authors: Wu SY, Lai MC
- journal: Appl Environ Microbiol
- DOI: 10.1128/AEM.01539-10
- year: 2010
- mesh: Archaeal Proteins/analysis, Base Composition, Culture Media, *Earthquakes, Genes, rRNA, Geologic Sediments/*microbiology, Methane/*metabolism, Methanobacterium/*classification/genetics/*isolation & purification/physiology, Methanosarcinaceae/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Taiwan
- topic2: Cultivation
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21019 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19953 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19953) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 88120 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID400193.1 |