General

@ref: 20646

BacDive-ID: 24160

DSM-Number: 27305

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanomethylovorans uponensis EK1 is an anaerobe, mesophilic archaeon that was isolated from wetland sediment.

NCBI tax id

• NCBI tax id: 1341726
• Matching level: species

strain history

doi: 10.13145/bacdive24160.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanosarcinales
• family: Methanosarcinaceae
• genus: Methanomethylovorans
• species: Methanomethylovorans uponensis
• full scientific name: Methanomethylovorans uponensis Cha et al. 2014

@ref: 20646

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanomethylovorans

species: Methanomethylovorans uponensis

full scientific name: Methanomethylovorans uponensis Cha et al. 2014

strain designation: EK1

type strain: yes

Morphology

colony morphology

• @ref: 20646
• incubation period: 3-7 days

Culture and growth conditions

culture medium

• @ref: 20646
• name: ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503)
• growth: yes
• link: https://mediadive.dsmz.de/medium/503
• composition: Name: ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503; with strain-specific modifications) Composition: Trimethylamine-HCl 1.99402 g/l Na2CO3 1.49551 g/l NaCl 0.997008 g/l Methanol 0.631705 g/l KCl 0.498505 g/l MgCl2 x 6 H2O 0.398803 g/l Na2S x 9 H2O 0.299103 g/l NH4Cl 0.249252 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149552 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498505 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l Nicotinic acid 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Calcium pantothenate 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water

culture temp

Physiology and metabolism

oxygen tolerance

• @ref: 20646
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

isolation source categories

Safety information

risk assessment

• @ref: 20646
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 20646
• description: Methanomethylovorans uponensis strain EK1 from South Korea 16S ribosomal RNA gene, partial sequence
• accession: KC876048
• length: 1387
• database: ena
• NCBI tax ID: 1341726

GC content

• @ref: 20646
• GC-content: 39.2
• method: thermal denaturation, midpoint method (Tm)

External links

@ref: 20646

culture collection no.: DSM 27305, JCM 19217, KCTC 4119, NBRC 109636

straininfo link

• @ref: 88116
• straininfo: 398372

literature

• topic: Phylogeny
• Pubmed-ID: 24000091
• title: Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment.
• authors: Cha IT, Min UG, Kim SJ, Yim KJ, Roh SW, Rhee SK
• journal: Antonie Van Leeuwenhoek
• DOI: 10.1007/s10482-013-0020-4
• year: 2013
• mesh: Base Composition, Carbon/metabolism, Cluster Analysis, DNA, Archaeal/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Energy Metabolism, Genes, rRNA, Geologic Sediments/*microbiology, Hydrogen-Ion Concentration, Methane/metabolism, Methanosarcinaceae/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidoreductases/genetics, Phylogeny, RNA, Archaeal/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, Temperature, Wetlands
• topic2: Metabolism

Reference