Strain identifier
BacDive ID: 24154
Type strain:
Species: Methanobacterium movilense
Strain Designation: MC-20
Strain history: D. Wagner MC-20.
NCBI tax ID(s): 1036678 (species)
General
@ref: 21009
BacDive-ID: 24154
DSM-Number: 26032
keywords: 16S sequence, Archaea, anaerobe, mesophilic
description: Methanobacterium movilense MC-20 is an anaerobe, mesophilic archaeon that was isolated from anaerobic sediment a of subsurface lake.
NCBI tax id
- NCBI tax id: 1036678
- Matching level: species
strain history
@ref | history |
---|---|
21009 | <- D. Wagner, Helmholtz Centre Potsdam, GFZ German Res. Centre for Geosciences, Germany; MC-20 <- D. Wagner {2004} |
67770 | D. Wagner MC-20. |
doi: 10.13145/bacdive24154.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanobacterium
- species: Methanobacterium movilense
- full scientific name: Methanobacterium movilense Schirmack et al. 2014
@ref: 21009
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanobacterium
species: Methanobacterium movilense
full scientific name: Methanobacterium movilense Schirmack et al. 2014
strain designation: MC-20
type strain: yes
Morphology
colony morphology
- @ref: 21009
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 21009
- name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
- growth: yes
- link: https://mediadive.dsmz.de/medium/119
- composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
21009 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 21009
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
---|---|---|---|---|---|---|---|
21009 | anaerobic sediment a of subsurface lake | Mangalia, Movile cave | Romania | ROU | Europe | 43.8167 | 28.55 |
67770 | Anoxic sediment of a subsurface lake in Movile Cave | Mangalia | Romania | ROU | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Lake (large) |
#Environmental | #Aquatic | #Sediment |
#Condition | #Anoxic (anaerobic) |
taxonmaps
- @ref: 69479
- File name: preview.99_192475.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_71054;97_92308;98_125467;99_192475&stattab=map
- Last taxonomy: Methanobacterium movilense subclade
- 16S sequence: JF812256
- Sequence Identity:
- Total samples: 134
- soil counts: 69
- aquatic counts: 54
- animal counts: 10
- plant counts: 1
Safety information
risk assessment
- @ref: 21009
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21009
- description: Methanobacterium movilense strain MC-20 from Romania 16S ribosomal RNA gene, partial sequence
- accession: JF812256
- length: 1347
- database: ena
- NCBI tax ID: 1036678
GC content
@ref | GC-content | method |
---|---|---|
21009 | 33.0 | high performance liquid chromatography (HPLC) |
67770 | 33 | high performance liquid chromatography (HPLC) |
External links
@ref: 21009
culture collection no.: DSM 26032, JCM 18470
straininfo link
- @ref: 88112
- straininfo: 398850
literature
- topic: Phylogeny
- Pubmed-ID: 24108325
- title: Methanobacterium movilense sp. nov., a hydrogenotrophic, secondary-alcohol-utilizing methanogen from the anoxic sediment of a subsurface lake.
- authors: Schirmack J, Mangelsdorf K, Ganzert L, Sand W, Hillebrand-Voiculescu A, Wagner D
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.057224-0
- year: 2013
- mesh: Base Composition, Caves/*microbiology, DNA, Archaeal/genetics, Geologic Sediments/*microbiology, Lakes/microbiology, Methanobacterium/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Romania, Sequence Analysis, DNA
- topic2: Genetics
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
21009 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26032 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26032) | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
88112 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398850.1 |