We are hiring!

Strain identifier

BacDive ID: 24154

Type strain: Yes

Species: Methanobacterium movilense

Strain Designation: MC-20

Strain history: D. Wagner MC-20.

NCBI tax ID(s): 1036678 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 7.1 (current version):
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 7.1 (current version)

General

@ref: 21009

BacDive-ID: 24154

DSM-Number: 26032

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanobacterium movilense MC-20 is an anaerobe, mesophilic archaeon that was isolated from anaerobic sediment a of subsurface lake.

NCBI tax id

  • NCBI tax id: 1036678
  • Matching level: species

strain history

doi: 10.13145/bacdive24154.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/euryarchaeota
  • domain: Archaea
  • phylum: Euryarchaeota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanobacterium
  • species: Methanobacterium movilense
  • full scientific name: Methanobacterium movilense Schirmack et al. 2014

@ref: 21009

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium movilense

full scientific name: Methanobacterium movilense Schirmack et al. 2014

strain designation: MC-20

type strain: yes

Morphology

colony morphology

  • @ref: 21009
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 21009
  • name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/119
  • composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

@refgrowthtypetemperaturerange
21009positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 21009
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
21009anaerobic sediment a of subsurface lakeMangalia, Movile caveRomaniaROUEurope43.816728.55
67770Anoxic sediment of a subsurface lake in Movile CaveMangaliaRomaniaROUEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_192475.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_71054;97_92308;98_125467;99_192475&stattab=map
  • Last taxonomy: Methanobacterium movilense subclade
  • 16S sequence: JF812256
  • Sequence Identity:
  • Total samples: 134
  • soil counts: 69
  • aquatic counts: 54
  • animal counts: 10
  • plant counts: 1

Safety information

risk assessment

  • @ref: 21009
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21009
  • description: Methanobacterium movilense strain MC-20 from Romania 16S ribosomal RNA gene, partial sequence
  • accession: JF812256
  • length: 1347
  • database: ena
  • NCBI tax ID: 1036678

GC content

@refGC-contentmethod
2100933.0high performance liquid chromatography (HPLC)
6777033high performance liquid chromatography (HPLC)

External links

@ref: 21009

culture collection no.: DSM 26032, JCM 18470

straininfo link

@refpassport
20218http://www.straininfo.net/strains/893380
20218http://www.straininfo.net/strains/893381

literature

  • topic: Phylogeny
  • Pubmed-ID: 24108325
  • title: Methanobacterium movilense sp. nov., a hydrogenotrophic, secondary-alcohol-utilizing methanogen from the anoxic sediment of a subsurface lake.
  • authors: Schirmack J, Mangelsdorf K, Ganzert L, Sand W, Hillebrand-Voiculescu A, Wagner D
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.057224-0
  • year: 2013
  • mesh: Base Composition, Caves/*microbiology, DNA, Archaeal/genetics, Geologic Sediments/*microbiology, Lakes/microbiology, Methanobacterium/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Romania, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.StrainInfo introduces electronic passports for microorganisms.10.1016/j.syapm.2013.11.002Syst Appl Microbiol. 37: 42-50 201424321274
21009Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26032Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26032)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/