Strain identifier
BacDive ID: 24137
Type strain:
Species: Ligilactobacillus faecis
Strain Designation: AFL 13-2, AFL13-2
Strain history: CIP <- 2014, DSMZ <- A. Endo,Stellenbosch Univ., Stellenbosch, South Africa: strain AFL13-2
NCBI tax ID(s): 762833 (species)
General
@ref: 20997
BacDive-ID: 24137
DSM-Number: 23956
keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped
description: Ligilactobacillus faecis AFL 13-2 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from faeces of jackal .
NCBI tax id
- NCBI tax id: 762833
- Matching level: species
strain history
@ref | history |
---|---|
20997 | <- A. Endo, Dept. Microbiology, Stellenbosch Univ., South Africa; AFL 13-2 |
67770 | A. Endo AFL13-2. |
119221 | CIP <- 2014, DSMZ <- A. Endo,Stellenbosch Univ., Stellenbosch, South Africa: strain AFL13-2 |
doi: 10.13145/bacdive24137.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Lactobacillaceae
- genus: Ligilactobacillus
- species: Ligilactobacillus faecis
- full scientific name: Ligilactobacillus faecis (Endo et al. 2013) Zheng et al. 2020
synonyms
- @ref: 20215
- synonym: Lactobacillus faecis
@ref: 20997
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Lactobacillaceae
genus: Ligilactobacillus
species: Ligilactobacillus faecis
full scientific name: Ligilactobacillus faecis (Endo et al. 2013) Zheng et al. 2020
strain designation: AFL 13-2, AFL13-2
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
31129 | positive | 5 µm | 0.8 µm | rod-shaped | no |
119221 | positive | rod-shaped | no |
pigmentation
- @ref: 31129
- production: yes
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
41600 | MEDIUM 40- for Lactobacillus and Leuconostoc | yes | Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g) | |
20997 | MRS MEDIUM (DSMZ Medium 11) | yes | Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water | https://mediadive.dsmz.de/medium/11 |
119221 | CIP Medium 40 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
20997 | positive | growth | 37 | mesophilic |
31129 | positive | growth | 20-50 | |
41600 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
culture pH
- @ref: 31129
- ability: positive
- type: growth
- pH: 5.0-8.0
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
20997 | microaerophile |
31129 | facultative anaerobe |
halophily
- @ref: 31129
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31129 | 28260 | galactose | + | carbon source |
31129 | 17716 | lactose | + | carbon source |
31129 | 29864 | mannitol | + | carbon source |
31129 | 28053 | melibiose | + | carbon source |
31129 | 506227 | N-acetylglucosamine | + | carbon source |
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | 24265 | gluconate | - | builds acid from |
68371 | 18403 | L-arabitol | - | builds acid from |
68371 | 18333 | D-arabitol | - | builds acid from |
68371 | 18287 | L-fucose | - | builds acid from |
68371 | 28847 | D-fucose | - | builds acid from |
68371 | 16443 | D-tagatose | - | builds acid from |
68371 | 62318 | D-lyxose | - | builds acid from |
68371 | 32528 | turanose | - | builds acid from |
68371 | 28066 | gentiobiose | + | builds acid from |
68371 | 17151 | xylitol | - | builds acid from |
68371 | 28087 | glycogen | - | builds acid from |
68371 | 28017 | starch | - | builds acid from |
68371 | 16634 | raffinose | + | builds acid from |
68371 | 6731 | melezitose | - | builds acid from |
68371 | 15443 | inulin | - | builds acid from |
68371 | 27082 | trehalose | - | builds acid from |
68371 | 17992 | sucrose | + | builds acid from |
68371 | 28053 | melibiose | + | builds acid from |
68371 | 17716 | lactose | + | builds acid from |
68371 | 17306 | maltose | + | builds acid from |
68371 | 17057 | cellobiose | + | builds acid from |
68371 | 17814 | salicin | - | builds acid from |
68371 | 4853 | esculin | - | builds acid from |
68371 | 18305 | arbutin | - | builds acid from |
68371 | 27613 | amygdalin | - | builds acid from |
68371 | 59640 | N-acetylglucosamine | + | builds acid from |
68371 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
68371 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
68371 | 17924 | D-sorbitol | - | builds acid from |
68371 | 16899 | D-mannitol | - | builds acid from |
68371 | 17268 | myo-inositol | - | builds acid from |
68371 | 16813 | galactitol | - | builds acid from |
68371 | 62345 | L-rhamnose | - | builds acid from |
68371 | 17266 | L-sorbose | - | builds acid from |
68371 | 16024 | D-mannose | + | builds acid from |
68371 | 15824 | D-fructose | + | builds acid from |
68371 | 17634 | D-glucose | + | builds acid from |
68371 | 12936 | D-galactose | + | builds acid from |
68371 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
68371 | 15963 | ribitol | - | builds acid from |
68371 | 65328 | L-xylose | - | builds acid from |
68371 | 65327 | D-xylose | - | builds acid from |
68371 | 16988 | D-ribose | - | builds acid from |
68371 | 30849 | L-arabinose | - | builds acid from |
68371 | 17108 | D-arabinose | - | builds acid from |
68371 | 17113 | erythritol | - | builds acid from |
68371 | 17754 | glycerol | - | builds acid from |
API 50CHac
@ref | Q | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20997 | - | - | - | - | - | - | - | - | - | - | + | + | + | + | - | - | - | - | - | - | - | - | + | - | - | - | - | + | + | + | + | + | - | - | - | + | - | - | - | + | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent |
---|---|---|---|---|---|---|
20997 | faeces of jackal (Canis mesomelas) | Canis mesomelas | Cape Town | South Africa | ZAF | Africa |
67770 | Feces of a jackal (Canis mesomelas) | Canis mesomelas | Cape Town | South Africa | ZAF | Africa |
119221 | Faeces of jackal, Canis mesolelas | Cape Town | South Africa | ZAF | Africa |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Canidae (Dog) |
#Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
20997 | 1 | Risk group (German classification) |
119221 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20997 | Lactobacillus faecis gene for 16S ribosomal RNA, partial sequence, strain: AFL13-2 | AB812750 | 1545 | ena | 762833 |
67770 | Lactobacillus faecis JCM 17300 gene for 16S rRNA, partial sequence | LC519991 | 1486 | ena | 762833 |
GC content
@ref | GC-content | method |
---|---|---|
20997 | 41.1 | |
67770 | 41.1 | high performance liquid chromatography (HPLC) |
External links
@ref: 20997
culture collection no.: DSM 23956, CIP 110788, JCM 17300
straininfo link
- @ref: 88095
- straininfo: 391584
literature
- topic: Phylogeny
- Pubmed-ID: 23907223
- title: Lactobacillus faecis sp. nov., isolated from animal faeces.
- authors: Endo A, Irisawa T, Futagawa-Endo Y, Salminen S, Ohkuma M, Dicks L
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.052985-0
- year: 2013
- mesh: Animals, Canidae/microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Raccoons/microbiology, Sequence Analysis, DNA, South Africa
- topic2: Genetics
Reference
@id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
20997 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23956 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23956) | |||
31129 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27458 | 28776041 | |
41600 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/30600 | ||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
88095 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID391584.1 | |||
119221 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110788 | Collection of Institut Pasteur (CIP 110788) |