Strain identifier

BacDive ID: 24137

Type strain: Yes

Species: Ligilactobacillus faecis

Strain Designation: AFL 13-2, AFL13-2

Strain history: CIP <- 2014, DSMZ <- A. Endo,Stellenbosch Univ., Stellenbosch, South Africa: strain AFL13-2

NCBI tax ID(s): 762833 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 20997

BacDive-ID: 24137

DSM-Number: 23956

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Ligilactobacillus faecis AFL 13-2 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from faeces of jackal .

NCBI tax id

  • NCBI tax id: 762833
  • Matching level: species

strain history

@refhistory
20997<- A. Endo, Dept. Microbiology, Stellenbosch Univ., South Africa; AFL 13-2
67770A. Endo AFL13-2.
119221CIP <- 2014, DSMZ <- A. Endo,Stellenbosch Univ., Stellenbosch, South Africa: strain AFL13-2

doi: 10.13145/bacdive24137.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Ligilactobacillus
  • species: Ligilactobacillus faecis
  • full scientific name: Ligilactobacillus faecis (Endo et al. 2013) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus faecis

@ref: 20997

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Ligilactobacillus

species: Ligilactobacillus faecis

full scientific name: Ligilactobacillus faecis (Endo et al. 2013) Zheng et al. 2020

strain designation: AFL 13-2, AFL13-2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31129positive5 µm0.8 µmrod-shapedno
119221positiverod-shapedno

pigmentation

  • @ref: 31129
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41600MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
20997MRS MEDIUM (DSMZ Medium 11)yesName: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled waterhttps://mediadive.dsmz.de/medium/11
119221CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
20997positivegrowth37mesophilic
31129positivegrowth20-50
41600positivegrowth37mesophilic
67770positivegrowth37mesophilic

culture pH

  • @ref: 31129
  • ability: positive
  • type: growth
  • pH: 5.0-8.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
20997microaerophile
31129facultative anaerobe

halophily

  • @ref: 31129
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3112928260galactose+carbon source
3112917716lactose+carbon source
3112929864mannitol+carbon source
3112928053melibiose+carbon source
31129506227N-acetylglucosamine+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose+builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
20997----------++++--------+----+++++---+---+----------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
20997faeces of jackal (Canis mesomelas)Canis mesomelasCape TownSouth AfricaZAFAfrica
67770Feces of a jackal (Canis mesomelas)Canis mesomelasCape TownSouth AfricaZAFAfrica
119221Faeces of jackal, Canis mesolelasCape TownSouth AfricaZAFAfrica

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
209971Risk group (German classification)
1192211Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20997Lactobacillus faecis gene for 16S ribosomal RNA, partial sequence, strain: AFL13-2AB8127501545ena762833
67770Lactobacillus faecis JCM 17300 gene for 16S rRNA, partial sequenceLC5199911486ena762833

GC content

@refGC-contentmethod
2099741.1
6777041.1high performance liquid chromatography (HPLC)

External links

@ref: 20997

culture collection no.: DSM 23956, CIP 110788, JCM 17300

straininfo link

  • @ref: 88095
  • straininfo: 391584

literature

  • topic: Phylogeny
  • Pubmed-ID: 23907223
  • title: Lactobacillus faecis sp. nov., isolated from animal faeces.
  • authors: Endo A, Irisawa T, Futagawa-Endo Y, Salminen S, Ohkuma M, Dicks L
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.052985-0
  • year: 2013
  • mesh: Animals, Canidae/microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Raccoons/microbiology, Sequence Analysis, DNA, South Africa
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20997Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-23956Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23956)
31129Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172745828776041
41600Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/30600
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
88095Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID391584.1
119221Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110788Collection of Institut Pasteur (CIP 110788)