Strain identifier
BacDive ID: 24121
Type strain: 
Species: Mobilitalea sibirica
Strain Designation: P3M-3
Strain history: <- DSM <- OA Podosocorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia, P3M-3
NCBI tax ID(s): 1462919 (species)
version 8.1 (current version)
General
@ref: 21347
BacDive-ID: 24121
DSM-Number: 26468
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, motile, rod-shaped
description: Mobilitalea sibirica P3M-3 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from microbial mat in a wooden bath flooded with hot water from a 2775 m-deep well.
NCBI tax id
- NCBI tax id: 1462919
- Matching level: species
strain history
| @ref | history |
|---|---|
| 21347 | <- O. A. Podosocorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; P3M-3 <- O. A. Podosokorskaya {2009} |
| 67771 | <- DSM <- OA Podosocorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia, P3M-3 |
doi: 10.13145/bacdive24121.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Lachnospiraceae
- genus: Mobilitalea
- species: Mobilitalea sibirica
- full scientific name: Mobilitalea sibirica Podosokorskaya et al. 2014
@ref: 21347
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Lachnospiraceae
genus: Mobilitalea
species: Mobilitalea sibirica
full scientific name: Mobilitalea sibirica Podosokorskaya et al. 2014
strain designation: P3M-3
type strain: yes
Morphology
cell morphology
| @ref | cell shape | motility | gram stain | confidence |
|---|---|---|---|---|
| 67771 | rod-shaped | yes | ||
| 67771 | positive | |||
| 69480 | yes | 91.325 | ||
| 69480 | positive | 99.999 |
Culture and growth conditions
culture medium
- @ref: 21347
- name: DESULFOFARCIMEN MEDIUM (DSMZ Medium 124)
- growth: yes
- link: https://mediadive.dsmz.de/medium/124
- composition: Name: DESULFOFARCIMEN MEDIUM (DSMZ Medium 124) Composition: NaHCO3 4.48654 g/l Na2SO4 2.83151 g/l Na-acetate 1.39581 g/l Na-butyrate 1.39581 g/l NaCl 1.1665 g/l Yeast extract 0.997009 g/l MgCl2 x 6 H2O 0.398804 g/l Na2S x 9 H2O 0.398804 g/l KCl 0.299103 g/l NH4Cl 0.269192 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Thiamine HCl 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 21347 | positive | growth | 37 | mesophilic |
| 67771 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 21347 | anaerobe | |
| 67771 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
| @ref | type of spore | spore formation | confidence |
|---|---|---|---|
| 67771 | spore | yes | |
| 69481 | yes | 100 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 21347 | microbial mat in a wooden bath flooded with hot water from a 2775 m-deep well | Tomsk region, Parabel' district (58° 50' 05.3'' N 81° 30' 07.5'' E) | Russia | RUS | Asia | 58.8348 | 81.5021 |
| 67771 | From microbial mat in a wooden bath flooded with hot water from a 2775 m-deep well | Parabel' district, Tomsk region(Siberia) | Russia | RUS | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | |
| #Environmental | #Microbial community | #Microbial mat |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_156727.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_33;96_4278;97_48331;98_103298;99_156727&stattab=map
- Last taxonomy: Mobilitalea sibirica subclade
- 16S sequence: KF931641
- Sequence Identity:
- Total samples: 37
- soil counts: 4
- aquatic counts: 20
- animal counts: 13
Safety information
risk assessment
- @ref: 21347
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21347
- description: Mobilitalea sibirica strain P3M-3 16S ribosomal RNA gene, partial sequence
- accession: KF931641
- length: 1444
- database: ena
- NCBI tax ID: 1462919
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Mobilitalea sibirica DSM 26468 | GCA_015999265 | contig | ncbi | 1462919 |
| 66792 | Mobilitalea sibirica strain DSM 26468 | 1462919.3 | wgs | patric | 1462919 |
GC content
- @ref: 21347
- GC-content: 33.4
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | yes | 85.027 | yes |
| gram-positive | yes | 88.315 | yes |
| anaerobic | yes | 98.017 | no |
| aerobic | no | 97.425 | yes |
| halophile | no | 81.355 | no |
| spore-forming | yes | 91.574 | yes |
| thermophile | no | 81.852 | no |
| glucose-util | yes | 85.996 | no |
| flagellated | no | 53.496 | no |
| glucose-ferment | yes | 69.341 | no |
External links
@ref: 21347
culture collection no.: DSM 26468, KCTC 15522, VKM B-2804
straininfo link
- @ref: 88081
- straininfo: 407666
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24827706 | Mobilitalea sibirica gen. nov., sp. nov., a halotolerant polysaccharide-degrading bacterium. | Podosokorskaya OA, Bonch-Osmolovskaya EA, Beskorovaynyy AV, Toshchakov SV, Kolganova TV, Kublanov IV | Int J Syst Evol Microbiol | 10.1099/ijs.0.057109-0 | 2014 | Bacteria, Anaerobic/classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification, Hot Temperature, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, Polysaccharides/metabolism, RNA, Ribosomal, 16S/genetics, Russia, Salt Tolerance, Sequence Analysis, DNA, *Water Microbiology, Water Wells/*microbiology | Enzymology |
| Phylogeny | 26755447 | Mobilisporobacter senegalensis gen. nov., sp. nov., an anaerobic bacterium isolated from tropical shea cake. | Mbengue M, Thioye A, Labat M, Casalot L, Joseph M, Samb A, Ben Ali Gam Z | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000889 | 2016 | ||
| Genetics | 33795340 | Draft Genome Sequence of Mobilitalea sibirica Strain P3M-3(T), the Sole Representative of the Genus Mobilitalea. | Thieme N, Rettenmaier R, Liebl W, Zverlov VV | Microbiol Resour Announc | 10.1128/MRA.00129-21 | 2021 | ||
| Phylogeny | 34731077 | Variimorphobacter saccharofermentans gen. nov., sp. nov., a new member of the family Lachnospiraceae, isolated from a maize-fed biogas fermenter. | Rettenmaier R, Thieme N, Streubel J, Di Bello L, Kowollik ML, Huang L, Maus I, Klingl A, Liebl W, Zverlov VV | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005044 | 2021 | Bacterial Typing Techniques, Base Composition, *Biofuels/microbiology, Clostridiales/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Silage/*microbiology, *Zea mays/microbiology | Transcriptome |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21347 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26468 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26468) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 88081 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID407666.1 |