Strain identifier

BacDive ID: 24046

Type strain: Yes

Species: Christiangramia forsetii

Strain Designation: KT0803

Strain history: <- R. Amann, Max-Planck-Inst. Marine Microbiology, Bremen, Germany <- H. Eilers, Max-Planck-Inst. Marine Microbiology, Bremen, Germany; KT0803

NCBI tax ID(s): 411153 (species)

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General

@ref: 21083

BacDive-ID: 24046

DSM-Number: 17595

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, chemoorganotroph, mesophilic, Gram-negative, motile, rod-shaped, colony-forming, pigmented

description: Christiangramia forsetii KT0803 is an obligate aerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and has a orange–yellow pigmentation.

NCBI tax id

  • NCBI tax id: 411153
  • Matching level: species

strain history

  • @ref: 21083
  • history: <- R. Amann, Max-Planck-Inst. Marine Microbiology, Bremen, Germany <- H. Eilers, Max-Planck-Inst. Marine Microbiology, Bremen, Germany; KT0803

doi: 10.13145/bacdive24046.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Christiangramia
  • species: Christiangramia forsetii
  • full scientific name: Christiangramia forsetii (Panschin et al. 2017) Deshmukh and Oren 2023

  • synonyms

    • @ref: 20215
    • synonym: Gramella forsetii

@ref: 21083

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Gramella

species: Gramella forsetii

full scientific name: Gramella forsetii Panschin et al. 2017

strain designation: KT0803

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
25239negative1.5-3.0 µm0.6-0.8 µmrod-shapedyes
69480negative99.977

colony morphology

  • @ref: 25239
  • colony color: orange-yellow
  • colony shape: circular

pigmentation

@refproductioncolorname
25239yesorange–yellowCarotenoid pigments
25239noFlexirubin-type

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25239HaHa mediumyes
21083BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21083positivegrowth25mesophilic
25239positiveoptimum22.0-25.0
25239positivegrowth2.0-30.0

culture pH

@refabilitytypepH
25239positiveoptimum7.5
25239positivegrowth6.0-8.3

Physiology and metabolism

oxygen tolerance

  • @ref: 25239
  • oxygen tolerance: obligate aerobe

nutrition type

@reftype
25239chemoorganotroph
25239heterotroph

spore formation

@refspore formationconfidence
25239no
69481no100
69480no99.997

halophily

@refsalttested relationconcentrationgrowth
25239NaCloptimum2.0-3.0 %
25239NaClgrowth0.13-6.0 %(w/v)positive

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
25239286442-oxopentanoate-respiration
25239181014-hydroxyphenylacetic acid-respiration
252392509agar-hydrolysis
2523922605arabinitol-builds acid from
2523928427arabinoxylan-hydrolysis
252393435carrageenan-hydrolysis
2523962968cellulose-hydrolysis
2523917029chitin-hydrolysis
2523916261chitosan-hydrolysis
2523916947citrate-builds acid from
2523915963ribitol-builds acid from
2523962318D-lyxose-builds acid from
2523916899D-mannitol-assimilation
2523916024D-mannose-builds acid from
2523916988D-ribose-builds acid from
2523917924D-sorbitol-assimilation
2523917924D-sorbitol-builds acid from
2523916443D-tagatose-builds acid from
2523923652dextrin-respiration
2523933984fucose-builds acid from
2523916813galactitol-builds acid from
2523927680galactomannan-hydrolysis
2523928066gentiobiose-builds acid from
2523924265gluconate-builds acid from
2523985249hydroxyethylcellulose-hydrolysis
2523917268myo-inositol-builds acid from
2523917196L-asparagine-assimilation
2523917196L-asparagine-respiration
2523962345L-rhamnose-assimilation
2523962345L-rhamnose-builds acid from
2523928053melibiose-builds acid from
2523951850methyl pyruvate-respiration
25239506227N-acetylglucosamine-builds acid from
2523917632nitrate-reduction
2523916301nitrite-reduction
2523917309pectin-hydrolysis
2523941865sebacic acid-respiration
2523927922sorbose-builds acid from
2523930031succinate-assimilation
2523917992sucrose-builds acid from
2523927897tryptophan-energy source
2523953426tween 80-hydrolysis
2523937166xylan-hydrolysis
2523918222xylose-builds acid from
25239376711,3-beta-d-glucan+hydrolysis
25239370543-hydroxybutyrate+respiration
25239182404-hydroxy-L-proline+respiration
25239167244-hydroxybutyrate+assimilation
25239167244-hydroxybutyrate+respiration
2523930089acetate+assimilation
2523930089acetate+respiration
2523915963ribitol+respiration
2523958187alginate+hydrolysis
2523940585alpha-cyclodextrin+assimilation
2523940585alpha-cyclodextrin+respiration
2523936219alpha-lactose+respiration
25239495055beta-cyclodextrin+assimilation
2523917057cellobiose+assimilation
2523917057cellobiose+builds acid from
2523916947citrate+assimilation
2523917057cellobiose+respiration
2523915824D-fructose+assimilation
2523915824D-fructose+respiration
2523915824D-fructose+builds acid from
2523912936D-galactose+assimilation
252398391D-gluconate+respiration
2523917784D-glucosaminic acid+respiration
2523917634D-glucose+assimilation
2523917634D-glucose+builds acid from
2523914314D-glucose 6-phosphate+respiration
2523915748D-glucuronate+respiration
2523916899D-mannitol+builds acid from
2523916024D-mannose+assimilation
2523916024D-mannose+respiration
2523916024D-mannose+builds acid from
2523932528turanose+builds acid from
2523923652dextrin+assimilation
2523916991dna+hydrolysis
252394853esculin+hydrolysis
2523915740formate+respiration
2523937165galactan+hydrolysis
2523928260galactose+builds acid from
2523916865gamma-aminobutyric acid+respiration
252395291gelatin+hydrolysis
2523928066gentiobiose+respiration
2523917754glycerol+assimilation
2523917754glycerol+builds acid from
2523928087glycogen+respiration
2523928087glycogen+builds acid from
2523917596inosine+respiration
2523917268myo-inositol+assimilation
2523917268myo-inositol+respiration
2523930849L-arabinose+assimilation
2523930849L-arabinose+builds acid from
2523929985L-glutamate+respiration
2523962345L-rhamnose+respiration
2523924996lactate+assimilation
2523917716lactose+assimilation
2523917716lactose+builds acid from
252396359lactulose+respiration
252396364laminarin+hydrolysis
2523917306maltose+assimilation
2523917306maltose+builds acid from
2523928053melibiose+assimilation
2523928053melibiose+respiration
2523975146monomethyl succinate+respiration
2523928037N-acetylgalactosamine+respiration
25239506227N-acetylglucosamine+assimilation
2523973784glycyl-l-glutamate+respiration
2523916634raffinose+assimilation
2523916634raffinose+builds acid from
2523928017starch+hydrolysis
25239143136succinamate+respiration
2523930031succinate+respiration
2523917992sucrose+assimilation
2523917992sucrose+respiration
2523917992sucrose+builds acid from
2523917748thymidine+respiration
2523927082trehalose+assimilation
2523927082trehalose+respiration
2523927082trehalose+builds acid from
2523932528turanose+respiration
2523953423tween 40+hydrolysis
2523953423tween 40+respiration
2523953426tween 80+respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
2523948923erythromycinyesyes15 µg/mL
2523917076streptomycinyesyes10.0-50.0 µg/mL
2523927902tetracyclineyesyes30 µg/mL
2523928001vancomycinyesyes30 µg/mL
2523928971ampicillinyesyes10 µg/mL
2523928077rifampicinyesyes5.0-30.0 µg/mL

metabolite production

  • @ref: 25239
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 25239
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
25239acid phosphatase+3.1.3.2
25239alkaline phosphatase+3.1.3.1
25239alpha-chymotrypsin-3.4.21.1
25239alpha-fucosidase-3.2.1.51
25239alpha-galactosidase-3.2.1.22
25239alpha-glucosidase+3.2.1.20
25239alpha-mannosidase-3.2.1.24
25239beta-galactosidase-3.2.1.23
25239beta-glucosidase-3.2.1.21
25239beta-glucuronidase-3.2.1.31
25239catalase+1.11.1.6
25239cystine arylamidase+3.4.11.3
25239cytochrome oxidase+1.9.3.1
25239esterase (C 4)+
25239esterase Lipase (C 8)+
25239leucine arylamidase+3.4.11.1
25239lipase (C 14)+
25239N-acetyl-beta-glucosaminidase+3.2.1.52
25239naphthol-AS-BI-phosphohydrolase+
25239trypsin-3.4.21.4
25239tryptophan deaminase-4.1.99.1
25239valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    25239C15:0 anteiso5.9
    25239anteiso-C17:1ω8c3.2
    25239C15:06.4
    25239C15:0 2OH3.2
    25239C15:1ω6c2.9
    25239C16:1ω7c8.1
    25239C17:0 2OH3.6
    25239C17:1ω6c5.5
    25239C17:1ω8c1.9
    25239C15:0 iso15.2
    25239iso-C15:0 2-OH6.8
    25239C15:0 iso 3OH1.9
    25239C16:0 iso2.1
    25239C16:0 iso 3OH2.3
    25239C16:1 iso H1.6
    25239C17:0 iso 3OH10.2
    25239iso-C17:1ω7c7.2
    25239Unknown (ECL) 13.5663.714
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine broth (DSMZ medium 514)
  • agar/liquid: liquid
  • incubation temperature: 25
  • incubation time: 2
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA 40
  • system: MIS MIDI
  • method/protocol: Kämpfer & Kroppenstedt 1996
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateenrichment cultureenrichment culture compositionenrichment culture temperature
21083coastal eutrophic surface seawaterNorth Sea, near the island of Helgoland, Station "Kabeltonne" (54° 09' N 7° 52' E, at a depth of 1 m)GermanyDEUEurope54.157.8667
25239coastal eutrophic surface waters (1 m depth)North Sea, near the island of Helgoland, Station "Kabeltonne" (54° 09' N 7° 52' E, at a depth of 1 m)GermanyDEUEurope547August 1999agar platesartificial seawater after Schut et al. [15], supplemented with a mixture of amino acids, carbohydrates, alcohols and carboxylic acids25

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Surface water

taxonmaps

  • @ref: 69479
  • File name: preview.99_3707.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_52;96_1604;97_1915;98_2834;99_3707&stattab=map
  • Last taxonomy: Gramella forsetii
  • 16S sequence: AF235117
  • Sequence Identity:
  • Total samples: 104
  • soil counts: 6
  • aquatic counts: 95
  • animal counts: 3

Safety information

risk assessment

  • @ref: 21083
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21083
  • description: Gramella forsetii KT0803 16S ribosomal RNA gene, partial sequence
  • accession: AF235117
  • length: 1436
  • database: ena
  • NCBI tax ID: 411154

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Christiangramia forsetii KT0803GCA_000060345completencbi411154
66792Christiangramia forsetii CGMCC 1.15422GCA_014642775scaffoldncbi411153
66792Gramella forsetii KT0803411154.11completepatric411154
66792Gramella forsetii strain CGMCC 1.15422411153.5wgspatric411153
66792Gramella forsetii KT0803639633025completeimg411154

GC content

@refGC-contentmethod
2523936.6genome sequence analysis
2108336.6sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno84.106yes
flagellatedno95.446no
gram-positiveno98.022yes
anaerobicno99.389yes
aerobicyes90.911yes
halophileyes54.274no
spore-formingno96.417yes
thermophileno99.045yes
glucose-utilyes87.216yes
glucose-fermentno91.006no

External links

@ref: 21083

culture collection no.: DSM 17595, CGMCC 1.15422

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism17107561Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter.Bauer M, Kube M, Teeling H, Richter M, Lombardot T, Allers E, Wurdemann CA, Quast C, Kuhl H, Knaust F, Woebken D, Bischof K, Mussmann M, Choudhuri JV, Meyer F, Reinhardt R, Amann RI, Glockner FOEnviron Microbiol10.1111/j.1462-2920.2006.01152.x2006Adaptation, Physiological, Adhesins, Bacterial/genetics, Flavobacteriaceae/enzymology/*genetics/metabolism, Genome/*genetics, Hydrolases/genetics, Molecular Weight, Organic Chemicals/metabolism, Peptide Hydrolases/genetics, Polymers/chemistry/*metabolism, Proteome/*genetics, Seawater/microbiology, Transferases/geneticsEnzymology
Phylogeny17488508Phylogeny and molecular signatures (conserved proteins and indels) that are specific for the Bacteroidetes and Chlorobi species.Gupta RS, Lorenzini EBMC Evol Biol10.1186/1471-2148-7-712007Amino Acid Sequence, Bacterial Proteins/*genetics, Bacteroidetes/*genetics, Chlorobi/*genetics, Conserved Sequence, *Genome, Bacterial, Mutation, Open Reading Frames, *Phylogeny, Sequence AlignmentGenetics
Genetics22750556The limitations of draft assemblies for understanding prokaryotic adaptation and evolution.Ricker N, Qian H, Fulthorpe RRGenomics10.1016/j.ygeno.2012.06.0092012*Adaptation, Biological, Bordetella bronchiseptica/genetics, Caulobacter/genetics, Chromosomes, Bacterial/genetics, Computational Biology/methods, Computer Simulation, Cupriavidus/genetics, DNA Transposable Elements, DNA, Bacterial/analysis/genetics, Databases, Genetic, *Evolution, Molecular, *Genome, Bacterial, Genomic Instability, Genomic Islands, Molecular Sequence Annotation, Prokaryotic Cells/*cytology, Rhodobacter sphaeroides/genetics, Sequence Alignment, Sequence Analysis, DNA/methodsTranscriptome
Metabolism24522261Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803.Kabisch A, Otto A, Konig S, Becher D, Albrecht D, Schuler M, Teeling H, Amann RI, Schweder TISME J10.1038/ismej.2014.42014Alginates/*metabolism, Bacteroidetes/*genetics/metabolism, Carbon Cycle/genetics, Gene Expression Regulation, Bacterial, *Genetic Loci, Glucans/metabolism, Glucuronic Acid/genetics/metabolism, Hexuronic Acids/metabolism, Membrane Proteins/*genetics/metabolism, North Sea, Polysaccharides/genetics/*metabolism, Seawater/*microbiology, Synteny
Phylogeny27902319Description of Gramella forsetii sp. nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of Gramella gaetbulicola Cho et al. 2011.Panschin I, Becher M, Verbarg S, Sproer C, Rohde M, Schuler M, Amann RI, Harder J, Tindall BJ, Hahnke RLInt J Syst Evol Microbiol10.1099/ijsem.0.0017002017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Germany, North Sea, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny36355042Gramella sediminis sp. nov., isolated from a tidal flat of the Yellow Sea.Niu HJ, Dong KS, Guan L, Sun LP, Wang Q, Zhang YJ, Li Y, Xia CQ, Pei CXInt J Syst Evol Microbiol10.1099/ijsem.0.0056212022RNA, Ribosomal, 16S/genetics, Phylogeny, *Seawater, Base Composition, Bacterial Typing Techniques, DNA, Bacterial/genetics, Sequence Analysis, DNA, *Fatty Acids/chemistry, Vitamin K 2, Glycoside Hydrolases/geneticsTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21083Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-17595Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17595)
25239I. B. Panschin, Mareike,Verbarg, Susanne,Spröer, Cathrin,Rohde, Manfred,Schüler, Margarete,Amann, Rudolf I,Harder, Jens,Tindall, Brian J,Hahnke, Richard LDescription of Gramella forsetii sp. nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of Gramella gaetbulicola Cho et al. 201110.1099/ijsem.0.001700IJSEM 67: 697-703 201727902319
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1