Strain identifier
BacDive ID: 24005
Type strain: 
Species: Enterococcus olivae
Strain Designation: IGG16.11
Strain history: <- A. Maldonado, CSIC, Inst. de la Grasa, Sevilla; IGG16.11 <- H. L. Padrós
NCBI tax ID(s): 1242999 (species)
version 8.1 (current version)
General
@ref: 20959
BacDive-ID: 24005
DSM-Number: 25431
keywords: 16S sequence, Bacteria, microaerophile, mesophilic
description: Enterococcus olivae IGG16.11 is a microaerophile, mesophilic bacterium that was isolated from spanish-style green olive fermentations.
NCBI tax id
- NCBI tax id: 1242999
- Matching level: species
strain history
- @ref: 20959
- history: <- A. Maldonado, CSIC, Inst. de la Grasa, Sevilla; IGG16.11 <- H. L. Padrós
doi: 10.13145/bacdive24005.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Enterococcaceae
- genus: Enterococcus
- species: Enterococcus olivae
- full scientific name: Enterococcus olivae Lucena-Padrós et al. 2014
@ref: 20959
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Enterococcaceae
genus: Enterococcus
species: Enterococcus olivae
full scientific name: Enterococcus olivae Lucena-Padrós et al. 2014
strain designation: IGG16.11
type strain: yes
Morphology
colony morphology
- @ref: 20959
- type of hemolysis: gamma
- incubation period: 1-2 days
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 20959 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
| 20959 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
culture temp
- @ref: 20959
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 20959
- oxygen tolerance: microaerophile
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68381 | 29016 | arginine | - | hydrolysis |
| 68381 | 16988 | D-ribose | - | builds acid from |
| 68381 | 16899 | D-mannitol | + | builds acid from |
| 68381 | 30911 | sorbitol | + | builds acid from |
| 68381 | 17716 | lactose | + | builds acid from |
| 68381 | 27082 | trehalose | + | builds acid from |
| 68381 | 16634 | raffinose | + | builds acid from |
| 68381 | 17992 | sucrose | + | builds acid from |
| 68381 | 30849 | L-arabinose | - | builds acid from |
| 68381 | 18333 | D-arabitol | + | builds acid from |
| 68381 | 40585 | alpha-cyclodextrin | + | builds acid from |
| 68381 | 606565 | hippurate | - | hydrolysis |
| 68381 | 28087 | glycogen | - | builds acid from |
| 68381 | 27941 | pullulan | - | builds acid from |
| 68381 | 17306 | maltose | + | builds acid from |
| 68381 | 28053 | melibiose | + | builds acid from |
| 68381 | 6731 | melezitose | - | builds acid from |
| 68381 | 320055 | methyl beta-D-glucopyranoside | - | builds acid from |
| 68381 | 16443 | D-tagatose | + | builds acid from |
| 68381 | 16199 | urea | - | hydrolysis |
metabolite production
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- production: no
metabolite tests
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 20959 | cytochrome-c oxidase | - | 1.9.3.1 |
| 68381 | urease | - | 3.5.1.5 |
| 68381 | beta-mannosidase | - | 3.2.1.25 |
| 68381 | glycyl tryptophan arylamidase | + | |
| 68381 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
| 68381 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
| 68381 | beta-galactosidase | - | 3.2.1.23 |
| 68381 | Alanyl-Phenylalanyl-Proline arylamidase | - | |
| 68381 | alkaline phosphatase | - | 3.1.3.1 |
| 68381 | alpha-galactosidase | + | 3.2.1.22 |
| 68381 | beta-glucuronidase | - | 3.2.1.31 |
| 68381 | beta-glucosidase | + | 3.2.1.21 |
| 68381 | arginine dihydrolase | - | 3.5.3.6 |
API rID32STR
| @ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 20959 | - | + | - | - | + | - | - | + | + | + | + | + | + | - | + | + | - | - | - | + | - | + | - | - | - | + | + | - | - | + | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 20959
- sample type: spanish-style green olive fermentations
- geographic location: Sevilla
- country: Spain
- origin.country: ESP
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Food production | #Vegetable (incl. Grains) |
| #Host | #Plants | #Tree |
| #Host Body-Site | #Plant | #Fruit (Seed) |
| #Engineered | #Food production | #Fermented |
Safety information
risk assessment
- @ref: 20959
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20959
- description: Enterococcus olivae strain IGG16.11 16S ribosomal RNA gene, partial sequence
- accession: JQ283454
- length: 1406
- database: ena
- NCBI tax ID: 1242999
GC content
- @ref: 20959
- GC-content: 40.4
- method: fluorimetric
External links
@ref: 20959
culture collection no.: DSM 25431, CCM 8603, CECT 8063
straininfo link
- @ref: 87980
- straininfo: 394401
literature
- topic: Phylogeny
- Pubmed-ID: 24812366
- title: Enterococcus olivae sp. nov., isolated from Spanish-style green-olive fermentations.
- authors: Lucena-Padros H, Gonzalez JM, Caballero-Guerrero B, Ruiz-Barba JL, Maldonado-Barragan A
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.062208-0
- year: 2014
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Enterococcus/*classification/genetics/isolation & purification, *Fermentation, *Food Microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Olea/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain
- topic2: Biotechnology
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 20959 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25431 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25431) | |
| 68381 | Automatically annotated from API rID32STR | |||
| 87980 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID394401.1 |