Strain identifier
BacDive ID: 24004
Type strain:
Species: Enterococcus diestrammenae
Strain Designation: ORL-24
Strain history: J.-W. Bae ORL-24.
NCBI tax ID(s): 1155073 (species)
General
@ref: 20958
BacDive-ID: 24004
DSM-Number: 29072
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, coccus-shaped
description: Enterococcus diestrammenae ORL-24 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from gut of the camel cricket Diestrammena coreana.
NCBI tax id
- NCBI tax id: 1155073
- Matching level: species
strain history
@ref | history |
---|---|
20958 | <- JCM <- J.-W. Bae, Kyung Hee Univ., Seoul, Republic of Korea; ORL-24 |
67770 | J.-W. Bae ORL-24. |
doi: 10.13145/bacdive24004.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Enterococcaceae
- genus: Enterococcus
- species: Enterococcus diestrammenae
- full scientific name: Enterococcus diestrammenae Kim et al. 2013
@ref: 20958
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Enterococcaceae
genus: Enterococcus
species: Enterococcus diestrammenae
full scientific name: Enterococcus diestrammenae Kim et al. 2013
strain designation: ORL-24
type strain: yes
Morphology
cell morphology
- @ref: 31026
- gram stain: positive
- cell length: 1.15 µm
- cell width: 1.15 µm
- cell shape: coccus-shaped
- motility: no
colony morphology
- @ref: 20958
- type of hemolysis: alpha/beta
- hemolysis ability: 1
- incubation period: 1-2 days
pigmentation
- @ref: 31026
- production: yes
Culture and growth conditions
culture medium
- @ref: 20958
- name: MRS MEDIUM (DSMZ Medium 11)
- growth: yes
- link: https://mediadive.dsmz.de/medium/11
- composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
20958 | positive | growth | 37 | mesophilic |
31026 | positive | growth | 10-37 | |
31026 | positive | optimum | 33.5 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
31026 | positive | growth | 6.0-10.0 | alkaliphile |
31026 | positive | optimum | 8 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
20958 | anaerobe |
31026 | facultative anaerobe |
spore formation
- @ref: 31026
- spore formation: no
halophily
- @ref: 31026
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-5 %
observation
- @ref: 31026
- observation: aggregates in clumps
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31026 | 28644 | 2-oxopentanoate | + | carbon source |
31026 | 24996 | lactate | + | carbon source |
31026 | 15361 | pyruvate | + | carbon source |
31026 | 51850 | methyl pyruvate | + | carbon source |
31026 | 17748 | thymidine | + | carbon source |
31026 | 16704 | uridine | + | carbon source |
68381 | 29016 | arginine | - | hydrolysis |
68381 | 16899 | D-mannitol | - | builds acid from |
68381 | 30911 | sorbitol | - | builds acid from |
68381 | 27082 | trehalose | + | builds acid from |
68381 | 16634 | raffinose | - | builds acid from |
68381 | 17992 | sucrose | - | builds acid from |
68381 | 30849 | L-arabinose | + | builds acid from |
68381 | 18333 | D-arabitol | - | builds acid from |
68381 | 40585 | alpha-cyclodextrin | + | builds acid from |
68381 | 606565 | hippurate | - | hydrolysis |
68381 | 28087 | glycogen | - | builds acid from |
68381 | 27941 | pullulan | - | builds acid from |
68381 | 17306 | maltose | + | builds acid from |
68381 | 28053 | melibiose | - | builds acid from |
68381 | 6731 | melezitose | - | builds acid from |
68381 | 320055 | methyl beta-D-glucopyranoside | + | builds acid from |
68381 | 16443 | D-tagatose | - | builds acid from |
68381 | 16199 | urea | - | hydrolysis |
metabolite production
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- production: no
metabolite tests
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
20958 | catalase | - | 1.11.1.6 |
20958 | cytochrome-c oxidase | - | 1.9.3.1 |
31026 | acid phosphatase | + | 3.1.3.2 |
68381 | urease | - | 3.5.1.5 |
68381 | beta-mannosidase | - | 3.2.1.25 |
68381 | glycyl tryptophan arylamidase | + | |
68381 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68381 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68381 | beta-galactosidase | + | 3.2.1.23 |
68381 | Alanyl-Phenylalanyl-Proline arylamidase | - | |
68381 | alkaline phosphatase | - | 3.1.3.1 |
68381 | alpha-galactosidase | - | 3.2.1.22 |
68381 | beta-glucuronidase | - | 3.2.1.31 |
68381 | beta-glucosidase | + | 3.2.1.21 |
68381 | arginine dihydrolase | - | 3.5.3.6 |
API rID32STR
@ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20958 | - | + | + | - | - | - | +/- | - | - | +/- | + | - | - | + | - | + | - | - | + | - | + | + | - | - | - | + | - | - | + | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | country | origin.country | continent |
---|---|---|---|---|---|
20958 | gut of the camel cricket Diestrammena coreana | Diestrammena coreana | Republic of Korea | KOR | Asia |
67770 | Gut of the camel cricket |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Arthropoda | #Insecta |
#Host Body-Site | #Gastrointestinal tract | #Stomach |
Safety information
risk assessment
- @ref: 20958
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20958 | Enterococcus diestrammenae strain ORL-24 16S ribosomal RNA gene, partial sequence | JQ650245 | 1439 | ena | 1155073 |
67770 | Enterococcus diestrammenae JCM 18359 gene for 16S rRNA, partial sequence | LC521987 | 1489 | ena | 1155073 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Enterococcus diestrammenae strain DSM 29072 | 1155073.5 | wgs | patric | 1155073 |
66792 | Enterococcus diestrammenae DSM 29072 | 2901213754 | draft | img | 1155073 |
GC content
@ref | GC-content | method |
---|---|---|
31026 | 41.3 | |
67770 | 41.3 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 93.86 | yes |
gram-positive | yes | 93.596 | no |
anaerobic | no | 95.407 | no |
aerobic | no | 96.609 | yes |
halophile | yes | 73.43 | no |
spore-forming | no | 88.308 | yes |
flagellated | no | 97.397 | no |
thermophile | no | 98.794 | yes |
glucose-util | yes | 89.431 | no |
glucose-ferment | yes | 85.04 | no |
External links
@ref: 20958
culture collection no.: DSM 29072, JCM 18359, KACC 16708
straininfo link
- @ref: 87979
- straininfo: 402198
literature
- topic: Phylogeny
- Pubmed-ID: 23907226
- title: Enterococcus diestrammenae sp. nov., isolated from the gut of Diestrammena coreana.
- authors: Kim JY, Shin NR, Na HK, Hyun DW, Whon TW, Kim PS, Yun JH, Bae JW
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.050062-0
- year: 2013
- mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Enterococcus/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Gastrointestinal Tract/*microbiology, Genes, Bacterial, Gryllidae/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA
- topic2: Genetics
Reference
@id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
20958 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29072 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29072) | |||
31026 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27356 | 28776041 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68381 | Automatically annotated from API rID32STR | |||||
87979 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402198.1 |