Strain identifier

BacDive ID: 24004

Type strain: Yes

Species: Enterococcus diestrammenae

Strain Designation: ORL-24

Strain history: J.-W. Bae ORL-24.

NCBI tax ID(s): 1155073 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 20958

BacDive-ID: 24004

DSM-Number: 29072

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, coccus-shaped

description: Enterococcus diestrammenae ORL-24 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from gut of the camel cricket Diestrammena coreana.

NCBI tax id

  • NCBI tax id: 1155073
  • Matching level: species

strain history

@refhistory
20958<- JCM <- J.-W. Bae, Kyung Hee Univ., Seoul, Republic of Korea; ORL-24
67770J.-W. Bae ORL-24.

doi: 10.13145/bacdive24004.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus diestrammenae
  • full scientific name: Enterococcus diestrammenae Kim et al. 2013

@ref: 20958

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus diestrammenae

full scientific name: Enterococcus diestrammenae Kim et al. 2013

strain designation: ORL-24

type strain: yes

Morphology

cell morphology

  • @ref: 31026
  • gram stain: positive
  • cell length: 1.15 µm
  • cell width: 1.15 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 20958
  • type of hemolysis: alpha/beta
  • hemolysis ability: 1
  • incubation period: 1-2 days

pigmentation

  • @ref: 31026
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 20958
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
20958positivegrowth37mesophilic
31026positivegrowth10-37
31026positiveoptimum33.5mesophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
31026positivegrowth6.0-10.0alkaliphile
31026positiveoptimum8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
20958anaerobe
31026facultative anaerobe

spore formation

  • @ref: 31026
  • spore formation: no

halophily

  • @ref: 31026
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

observation

  • @ref: 31026
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
31026286442-oxopentanoate+carbon source
3102624996lactate+carbon source
3102615361pyruvate+carbon source
3102651850methyl pyruvate+carbon source
3102617748thymidine+carbon source
3102616704uridine+carbon source
6838129016arginine-hydrolysis
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
20958catalase-1.11.1.6
20958cytochrome-c oxidase-1.9.3.1
31026acid phosphatase+3.1.3.2
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
20958-++---+/---+/-+--+-+--+-++---+--+---

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinent
20958gut of the camel cricket Diestrammena coreanaDiestrammena coreanaRepublic of KoreaKORAsia
67770Gut of the camel cricket

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body-Site#Gastrointestinal tract#Stomach

Safety information

risk assessment

  • @ref: 20958
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20958Enterococcus diestrammenae strain ORL-24 16S ribosomal RNA gene, partial sequenceJQ6502451439ena1155073
67770Enterococcus diestrammenae JCM 18359 gene for 16S rRNA, partial sequenceLC5219871489ena1155073

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus diestrammenae strain DSM 290721155073.5wgspatric1155073
66792Enterococcus diestrammenae DSM 290722901213754draftimg1155073

GC content

@refGC-contentmethod
3102641.3
6777041.3thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno93.86yes
gram-positiveyes93.596no
anaerobicno95.407no
aerobicno96.609yes
halophileyes73.43no
spore-formingno88.308yes
flagellatedno97.397no
thermophileno98.794yes
glucose-utilyes89.431no
glucose-fermentyes85.04no

External links

@ref: 20958

culture collection no.: DSM 29072, JCM 18359, KACC 16708

straininfo link

  • @ref: 87979
  • straininfo: 402198

literature

  • topic: Phylogeny
  • Pubmed-ID: 23907226
  • title: Enterococcus diestrammenae sp. nov., isolated from the gut of Diestrammena coreana.
  • authors: Kim JY, Shin NR, Na HK, Hyun DW, Whon TW, Kim PS, Yun JH, Bae JW
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.050062-0
  • year: 2013
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Enterococcus/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Gastrointestinal Tract/*microbiology, Genes, Bacterial, Gryllidae/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20958Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29072Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29072)
31026Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172735628776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68381Automatically annotated from API rID32STR
87979Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402198.1