Strain identifier

BacDive ID: 2398

Type strain: Yes

Species: Chlorobaculum thiosulfatiphilum

Strain Designation: 6230

Culture col. no.: DSM 249

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General

@ref: 88

BacDive-ID: 2398

DSM-Number: 249

keywords: mesophilic, phototroph, anaerobe, Bacteria, 16S sequence, genome sequence, rod-shaped

description: Chlorobaculum thiosulfatiphilum 6230 is an anaerobe, phototroph, mesophilic bacterium that was isolated from tassajara hot spring.

strain history: <- N. Pfennig, 6230 deposited as (Chlorobium limicola f. thiosulfatophilum)

doi: 10.13145/bacdive2398.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/chlorobi
  • domain: Bacteria
  • phylum: Chlorobi
  • class: Chlorobia
  • order: Chlorobiales
  • family: Chlorobiaceae
  • genus: Chlorobaculum
  • species: Chlorobaculum thiosulfatiphilum
  • full scientific name: Chlorobaculum thiosulfatiphilum Imhoff 2003

@ref: 88

domain: Bacteria

phylum: Chlorobi

class: Chlorobea

order: Chlorobiales

family: Chlorobiaceae

genus: Chlorobaculum

species: Chlorobaculum thiosulfatiphilum

full scientific name: Chlorobaculum thiosulfatiphilum Imhoff 2003

strain designation: 6230

type strain: yes

Morphology

cell morphology

  • @ref: 23074
  • cell width: 0.7-1.1 µm
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

  • @ref: 88
  • name: PFENNIG'S MEDIUM II (modified 1988, for green sulfur bacteria) (DSMZ Medium 29), anaerobic in light
  • growth: yes
  • link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium29.pdf

culture temp

  • @ref: 88
  • growth: positive
  • type: growth
  • temperature: 25
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
88anaerobe
23074obligate anaerobe

nutrition type

  • @ref: 23074
  • type: phototroph

compound production

  • @ref: 88
  • compound: pyruvate synthase

metabolite utilization

  • @ref: 23074
  • Chebi-ID: 30411
  • metabolite: cobalamin
  • utilization activity: +
  • kind of utilization tested: growth

Isolation, sampling and environmental information

isolation

  • @ref: 88
  • sample type: tassajara hot spring
  • geographic location: California
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 88
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Chlorobaculum thiosulfatiphilum partial 16S rRNA-23S rRNA ITS, strain DSM 249AM049277479ena115852
23074Chlorobium limicola f. thiosulfatophilum 16S rRNA geneY081021419nuccore115852

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Chlorobaculum thiosulfatiphilum DSM 249GCA_006265165contigncbi115852
66792Chlorobaculum thiosulfatiphilum strain DSM 249115852.5wgspatric115852

GC content

@refGC-contentmethod
2307458.1Buoyant density centrifugation (BD)
8858.1

External links

@ref: 88

culture collection no.: DSM 249

straininfo link

  • @ref: 20218
  • passport: http://www.straininfo.net/strains/156533

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
88Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 249)https://www.dsmz.de/collection/catalogue/details/culture/DSM-249
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23074Johannes F. Imhoff10.1099/ijs.0.02403-0Phylogenetic taxonomy of the family Chlorobiaceae on the basis of 16S rRNA and fmo (Fenna-Matthews-Olson protein) gene sequencesIJSEM 53: 941-951 2003
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)