Strain identifier

BacDive ID: 2395

Type strain: Yes

Species: Chlorobaculum tepidum

Strain Designation: TLS

Strain history: <- M. T. Madigan

NCBI tax ID(s): 1097 (species)

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General

@ref: 4579

BacDive-ID: 2395

DSM-Number: 12025

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, phototroph, thermophilic

description: Chlorobaculum tepidum TLS is an anaerobe, phototroph, thermophilic bacterium that was isolated from acidic high sulfide hot springs.

NCBI tax id

  • NCBI tax id: 1097
  • Matching level: species

strain history

  • @ref: 4579
  • history: <- M. T. Madigan

doi: 10.13145/bacdive2395.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/chlorobiota
  • domain: Bacteria
  • phylum: Chlorobiota
  • class: Chlorobiia
  • order: Chlorobiales
  • family: Chlorobiaceae
  • genus: Chlorobaculum
  • species: Chlorobaculum tepidum
  • full scientific name: Chlorobaculum tepidum (Wahlund et al. 1996) Imhoff 2003
  • synonyms

    • @ref: 20215
    • synonym: Chlorobium tepidum

@ref: 4579

domain: Bacteria

phylum: Chlorobi

class: Chlorobia

order: Chlorobiales

family: Chlorobiaceae

genus: Chlorobaculum

species: Chlorobaculum tepidum

full scientific name: Chlorobaculum tepidum (Wahlund et al. 1996) Imhoff 2003

strain designation: TLS

type strain: yes

Morphology

cell morphology

  • @ref: 23074
  • cell width: 0.6-0.8 µm

Culture and growth conditions

culture medium

  • @ref: 4579
  • name: PFENNIG'S MEDIUM II (modified 1988, for green sulfur bacteria) (DSMZ Medium 29)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/29
  • composition: Name: PFENNIG'S MEDIUM II (DSMZ Medium 29; with strain-specific modifications) Composition: NaHCO3 1.5 g/l Thiosulfate 1.0 g/l Na2S x 9 H2O 0.592592 g/l MgSO4 x 7 H2O 0.52 g/l Ammonium chloride 0.364 g/l KCl 0.364 g/l KH2PO4 0.364 g/l Dextrose 0.26 g/l Pyruvic acid sodium salt 0.26 g/l Ammonium acetate 0.26 g/l CaCl2 x 2 H2O 0.25 g/l Yeast extract 0.25 g/l Resazurin 0.00225 g/l HCl 0.002002 g/l FeSO4 x 7 H2O 0.00156 g/l Vitamin B12 0.001 g/l H3BO3 0.000312 g/l CoCl2 x 6 H2O 0.0001976 g/l MnCl2 x 4 H2O 0.000104 g/l ZnCl2 7.28e-05 g/l Na2MoO4 x 2 H2O 3.744e-05 g/l NiCl2 x 6 H2O 2.496e-05 g/l CuCl2 x 2 H2O 2.08e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
4579positivegrowth47thermophilic
23074thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4579anaerobe
23074obligate anaerobe

nutrition type

  • @ref: 23074
  • type: phototroph

metabolite utilization

  • @ref: 23074
  • Chebi-ID: 26710
  • metabolite: sodium chloride
  • utilization activity: +
  • kind of utilization tested: growth

Isolation, sampling and environmental information

isolation

  • @ref: 4579
  • sample type: acidic high sulfide hot springs
  • geographic location: Travelodge Stream, Sulphur Bay, Rotorna
  • country: New Zealand
  • origin.country: NZL
  • continent: Australia and Oceania

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Acidic
#Condition#Sulfuric
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 4579
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 23074
  • description: Chlorobaculum tepidum strain TLS 16S ribosomal RNA gene, partial sequence
  • accession: M58468
  • length: 1450
  • database: nuccore
  • NCBI tax ID: 1097

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Chlorobaculum tepidum TLSGCA_000006985completencbi194439
66792Chlorobaculum tepidum TLS637000073completeimg194439

GC content

  • @ref: 23074
  • GC-content: 56.5
  • method: Buoyant density centrifugation (BD)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno91.792no
flagellatedno97.969no
gram-positiveno98.683no
anaerobicyes97.405yes
halophileno84.87no
spore-formingno95.459no
glucose-utilyes56.459no
aerobicno96.677no
thermophileyes72.719yes
glucose-fermentno83.129no

External links

@ref: 4579

culture collection no.: DSM 12025, ATCC 49652

straininfo link

  • @ref: 71950
  • straininfo: 49224

literature

  • topic: Phylogeny
  • Pubmed-ID: 12115058
  • title: Phylogeny of green sulfur bacteria on the basis of gene sequences of 16S rRNA and of the Fenna-Matthews-Olson protein.
  • authors: Alexander B, Andersen JH, Cox RP, Imhoff JF
  • journal: Arch Microbiol
  • DOI: 10.1007/s00203-002-0432-4
  • year: 2002
  • mesh: Amino Acid Sequence, *Bacterial Proteins, Base Sequence, Chlorobi/*classification/*genetics, DNA Primers/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Genes, Bacterial, *Light-Harvesting Protein Complexes, Molecular Sequence Data, Photosynthetic Reaction Center Complex Proteins/*genetics, *Phylogeny, Polymerase Chain Reaction, RNA, Bacterial/*genetics, RNA, Ribosomal, 16S/*genetics, Sequence Homology, Amino Acid
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4579Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12025)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12025
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23074Johannes F. Imhoff10.1099/ijs.0.02403-0Phylogenetic taxonomy of the family Chlorobiaceae on the basis of 16S rRNA and fmo (Fenna-Matthews-Olson protein) gene sequencesIJSEM 53: 941-951 200312892110
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71950Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49224.1StrainInfo: A central database for resolving microbial strain identifiers