Strain identifier

BacDive ID: 23914

Type strain: No

Species: Enterobacter sp.

Strain Designation: WS 20255

Strain history: <- Bakteriologisches Institut der Sueddeutschen Versuchs- und Forschungsanstalt fuer Milchwirtschaft, Freising-Weihenstephan, Germany; WS 20255

NCBI tax ID(s): 2747372 (species)

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General

@ref: 20543

BacDive-ID: 23914

DSM-Number: 30060

keywords: genome sequence, Bacteria, mesophilic, Gram-negative

description: Enterobacter sp. WS 20255 is a mesophilic, Gram-negative bacterium that was isolated from minced meat.

NCBI tax id

  • NCBI tax id: 2747372
  • Matching level: species

strain history

  • @ref: 20543
  • history: <- Bakteriologisches Institut der Sueddeutschen Versuchs- und Forschungsanstalt fuer Milchwirtschaft, Freising-Weihenstephan, Germany; WS 20255

doi: 10.13145/bacdive23914.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Enterobacter
  • species: Enterobacter sp.
  • full scientific name: Enterobacter Hormaeche and Edwards 1960 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Cloaca

@ref: 20543

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Enterobacter

species: Enterobacter sp.

full scientific name: Enterobacter sp.

strain designation: WS 20255

type strain: no

Morphology

cell morphology

@refgram stainconfidencemotility
69480negative99.994
6948097.458yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
20543COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
20543TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 20543
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.955

compound production

  • @ref: 20543
  • compound: restriction endonuclease EccI

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
20543+--++----+-++-++++++-

Isolation, sampling and environmental information

isolation

  • @ref: 20543
  • sample type: minced meat

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Meat

Safety information

risk assessment

  • @ref: 20543
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterobacter sp. DSM 30060GCA_013375955completencbi2747372
66792Enterobacter sp. DSM 300602747372.3completepatric2747372

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes87.975no
flagellatedno65.1no
gram-positiveno97.972no
anaerobicno96.18no
aerobicyes83.982no
halophileno86.786no
spore-formingno93.024no
thermophileno99.148no
glucose-utilyes95.024no
glucose-fermentyes93.359yes

External links

@ref: 20543

culture collection no.: DSM 30060

straininfo link

  • @ref: 87921
  • straininfo: 113620

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20543Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-30060Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 30060)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
87921Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID113620.1