Strain identifier
BacDive ID: 23792
Type strain:
Species: Alloscardovia macacae
Strain Designation: M8
Strain history: <- J. Killer, Inst. Animal Physiol. and Genetics, Czech Acad. Sci., Prague; M8
NCBI tax ID(s): 1160091 (species)
General
@ref: 21175
BacDive-ID: 23792
DSM-Number: 24762
keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, mesophilic, Gram-positive, ovoid-shaped
description: Alloscardovia macacae M8 is an obligate anaerobe, mesophilic, Gram-positive bacterium that was isolated from milk of a female macaque bred.
NCBI tax id
- NCBI tax id: 1160091
- Matching level: species
strain history
- @ref: 21175
- history: <- J. Killer, Inst. Animal Physiol. and Genetics, Czech Acad. Sci., Prague; M8
doi: 10.13145/bacdive23792.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Bifidobacteriales
- family: Bifidobacteriaceae
- genus: Alloscardovia
- species: Alloscardovia macacae
- full scientific name: Alloscardovia macacae Killer et al. 2013
@ref: 21175
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Bifidobacteriales
family: Bifidobacteriaceae
genus: Alloscardovia
species: Alloscardovia macacae
full scientific name: Alloscardovia macacae Killer et al. 2013
strain designation: M8
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
31073 | positive | 1.45 µm | 0.65 µm | ovoid-shaped | ||
69480 | no | 93.742 | ||||
69480 | positive | 100 |
pigmentation
- @ref: 31073
- production: yes
Culture and growth conditions
culture medium
- @ref: 21175
- name: MRS MEDIUM (DSMZ Medium 11)
- growth: yes
- link: https://mediadive.dsmz.de/medium/11
- composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
culture temp
- @ref: 21175
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
21175 | obligate anaerobe | |
31073 | anaerobe | |
69480 | anaerobe | 98.648 |
spore formation
- @ref: 69480
- spore formation: no
- confidence: 100
murein
- @ref: 21175
- murein short key: A11.31
- type: A4alpha L-Lys-D-Asp
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31073 | 22599 | arabinose | + | carbon source |
31073 | 17057 | cellobiose | + | carbon source |
31073 | 28260 | galactose | + | carbon source |
31073 | 17716 | lactose | + | carbon source |
31073 | 37684 | mannose | + | carbon source |
31073 | 28053 | melibiose | + | carbon source |
31073 | 18222 | xylose | + | carbon source |
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | 24265 | gluconate | + | builds acid from |
68371 | 18403 | L-arabitol | - | builds acid from |
68371 | 18333 | D-arabitol | - | builds acid from |
68371 | 18287 | L-fucose | - | builds acid from |
68371 | 28847 | D-fucose | - | builds acid from |
68371 | 16443 | D-tagatose | - | builds acid from |
68371 | 62318 | D-lyxose | - | builds acid from |
68371 | 32528 | turanose | + | builds acid from |
68371 | 28066 | gentiobiose | + | builds acid from |
68371 | 17151 | xylitol | - | builds acid from |
68371 | 28087 | glycogen | - | builds acid from |
68371 | 28017 | starch | - | builds acid from |
68371 | 16634 | raffinose | + | builds acid from |
68371 | 6731 | melezitose | + | builds acid from |
68371 | 17992 | sucrose | + | builds acid from |
68371 | 28053 | melibiose | + | builds acid from |
68371 | 17716 | lactose | + | builds acid from |
68371 | 17306 | maltose | + | builds acid from |
68371 | 17057 | cellobiose | + | builds acid from |
68371 | 17814 | salicin | + | builds acid from |
68371 | 4853 | esculin | + | builds acid from |
68371 | 18305 | arbutin | + | builds acid from |
68371 | 27613 | amygdalin | + | builds acid from |
68371 | 17924 | D-sorbitol | - | builds acid from |
68371 | 16899 | D-mannitol | - | builds acid from |
68371 | 17268 | myo-inositol | - | builds acid from |
68371 | 16813 | galactitol | - | builds acid from |
68371 | 62345 | L-rhamnose | - | builds acid from |
68371 | 17266 | L-sorbose | - | builds acid from |
68371 | 16024 | D-mannose | - | builds acid from |
68371 | 15824 | D-fructose | + | builds acid from |
68371 | 17634 | D-glucose | + | builds acid from |
68371 | 12936 | D-galactose | + | builds acid from |
68371 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
68371 | 15963 | ribitol | - | builds acid from |
68371 | 65328 | L-xylose | - | builds acid from |
68371 | 65327 | D-xylose | + | builds acid from |
68371 | 16988 | D-ribose | + | builds acid from |
68371 | 30849 | L-arabinose | + | builds acid from |
68371 | 17108 | D-arabinose | - | builds acid from |
68371 | 17113 | erythritol | - | builds acid from |
68371 | 17754 | glycerol | - | builds acid from |
API 50CHac
@ref | Q | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
21175 | - | - | - | - | + | + | + | - | - | - | + | + | + | - | - | - | - | - | - | - | +/- | +/- | +/- | + | + | + | + | + | + | + | + | + | +/- | +/- | + | + | - | - | - | + | + | - | - | - | - | - | - | + | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 21175
- sample type: milk of a female macaque bred
- geographic location: Central Bohemia
- country: Czech Republic
- origin.country: CZE
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Primates |
#Host Body Product | #Fluids | #Milk |
taxonmaps
- @ref: 69479
- File name: preview.99_109352.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_24;96_43791;97_56070;98_74241;99_109352&stattab=map
- Last taxonomy: Alloscardovia macacae subclade
- 16S sequence: JQ363617
- Sequence Identity:
- Total samples: 574
- soil counts: 4
- aquatic counts: 4
- animal counts: 566
Safety information
risk assessment
- @ref: 21175
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21175
- description: Alloscardovia macacae strain M8 16S ribosomal RNA gene, partial sequence
- accession: JQ363617
- length: 1436
- database: ena
- NCBI tax ID: 1160091
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Alloscardovia macacae DSM 24762 | GCA_002259705 | contig | ncbi | 1160091 |
66792 | Alloscardovia macacae strain DSM 24762 | 1160091.5 | wgs | patric | 1160091 |
66792 | Alloscardovia macacae DSM 24762 | 2788500374 | draft | img | 1160091 |
GC content
- @ref: 21175
- GC-content: 50.1
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 59 | no |
motile | no | 95.988 | no |
gram-positive | yes | 88.051 | no |
anaerobic | yes | 94.229 | no |
aerobic | no | 96.815 | yes |
halophile | no | 51.922 | no |
spore-forming | no | 96.855 | no |
flagellated | no | 96.704 | no |
thermophile | no | 96.923 | yes |
glucose-util | yes | 83.202 | no |
glucose-ferment | yes | 84.119 | no |
External links
@ref: 21175
culture collection no.: DSM 24762, CCM 7944
straininfo link
- @ref: 87823
- straininfo: 399068
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 23907220 | Alloscardovia macacae sp. nov., isolated from the milk of a macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov. | Killer J, Rockova S, Vlkova E, Rada V, Havlik J, Kopecny J, Bunesova V, Benada O, Kofronova O, Pechar R, Profousova I | Int J Syst Evol Microbiol | 10.1099/ijs.0.051326-0 | 2013 | Actinobacteria/*classification/genetics/isolation & purification, Aldehyde-Lyases/metabolism, Animals, Bacterial Typing Techniques, Base Composition, Carbohydrates/chemistry, DNA, Bacterial/genetics, Fatty Acids/chemistry, Female, Genes, Bacterial, Macaca mulatta/*microbiology, Milk/*microbiology, Molecular Sequence Data, Multilocus Sequence Typing, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Genetics |
Phylogeny | 31395120 | Alloscardovia theropitheci sp. nov., isolated from the faeces of gelada baboon, the 'bleeding heart' monkey (Theropithecus gelada). | Modesto M, Satti M, Watanabe K, Sciavilla P, Felis GE, Sandri C, Spiezio C, Arita M, Mattarelli P | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003584 | 2019 | Actinobacteria/*classification/isolation & purification, Aldehyde-Lyases, Animals, Animals, Zoo/microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, Italy, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Theropithecus/*microbiology | Transcriptome |
Reference
@id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed | journal |
---|---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||||
21175 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-24762 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24762) | ||||
31073 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27402 | 28776041 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
68371 | Automatically annotated from API 50CH acid | ||||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
87823 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID399068.1 |