Strain identifier

BacDive ID: 2378

Type strain: Yes

Species: Oerskovia turbata

Strain Designation: 27, AB 1476, N32

Culture col. no.: DSM 20577, ATCC 25835, CIP 100331, DSM 43880, JCM 3160, NCIB 10587

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8927

BacDive-ID: 2378

DSM-Number: 20577

keywords: mesophilic, Bacteria, 16S sequence, genome sequence, human pathogen

description: Oerskovia turbata 27 is a mesophilic human pathogen that was isolated from soil.

strain history: <- NCIB <- M. Goodfellow, N32 <- H. Lautrop, AB 1476 <- (J. Oerskov, 27)

doi: 10.13145/bacdive2378.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Micrococcales
  • family: Promicromonosporaceae
  • genus: Oerskovia
  • species: Oerskovia turbata
  • full scientific name: Oerskovia turbata (Erikson 1954) Prauser et al. 1970 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Nocardia turbata
    20215Cellulomonas turbata

@ref: 8927

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Promicromonosporaceae

genus: Oerskovia

species: Oerskovia turbata

full scientific name: Oerskovia turbata (Erikson 1954) Prauser et al. 1970 emend. Nouioui et al. 2018

strain designation: 27, AB 1476, N32

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18449Beige (1001)10-14 daysISP 2
18449Sulfur yellow (1016)10-14 daysISP 3
18449Broom yellow (1032)10-14 daysISP 4
18449Broom yellow (1032)10-14 daysISP 5
18449Broom yellow (1032)10-14 daysISP 6
18449Broom yellow (1032)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18449noISP 2
18449noISP 3
18449noISP 4
18449noISP 5
18449noISP 6
18449noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8927CORYNEBACTERIUM AGAR (DSMZ Medium 53)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium53.pdf
18449ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18449ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
18449ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18449ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18449ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18449ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
38084MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)

culture temp

@refgrowthtypetemperaturerange
18449positiveoptimum30mesophilic
8927positivegrowth30mesophilic
38084positivegrowth30mesophilic

Physiology and metabolism

halophily

  • @ref: 18449
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 7.5 %

murein

  • @ref: 8927
  • murein short key: A11.45
  • type: A4alpha L-Lys-L-Thr-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1844917234glucose+
1844922599arabinose+
1844917992sucrose+
1844918222xylose-
1844917268myo-inositol-
1844929864mannitol-
1844928757fructose+
1844926546rhamnose-
1844916634raffinose-
1844962968cellulose-

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18449+-++-++++--++++++++

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18449+++-++---++-+-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18449---------+-

Isolation, sampling and environmental information

isolation

  • @ref: 8927
  • sample type: soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 8927
  • pathogenicity human: yes
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218C.turbata 16S rRNA geneX838061451ena1322249
20218Oerskovia turbata NCIMB 10587 16S rDNAX794541510ena1322249

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Oerskovia turbata NRRL B-8019GCA_000718325contigncbi1713
66792Oerskovia turbata JCM3160GCA_004121315contigncbi1713
66792Oerskovia turbata strain JCM31601713.5wgspatric1713
66792Oerskovia turbata strain NRRL B-80191713.3wgspatric1713

GC content

  • @ref: 8927
  • GC-content: 70.5

External links

@ref: 8927

culture collection no.: DSM 20577, ATCC 25835, CIP 100331, DSM 43880, JCM 3160, NCIB 10587

straininfo link

@refpassport
20218http://www.straininfo.net/strains/52048
20218http://www.straininfo.net/strains/52046
20218http://www.straininfo.net/strains/149749
20218http://www.straininfo.net/strains/52049
20218http://www.straininfo.net/strains/52055
20218http://www.straininfo.net/strains/52058

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8927Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20577)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20577
18449Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20577.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38084Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11784
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)