Strain identifier
BacDive ID: 23771
Type strain:
Species: Thermolongibacillus kozakliensis
Strain Designation: E173a
Strain history: <- A. Coleri Cihan, Ankara Univ., Turkey; E173a <- A. Coleri Cihan, Ankara Univ., Turkey; E173a
NCBI tax ID(s): 575186 (species)
General
@ref: 21302
BacDive-ID: 23771
DSM-Number: 24978
keywords: 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped
description: Thermolongibacillus kozakliensis E173a is an aerobe, spore-forming, thermophilic bacterium that was isolated from soil beside a hotspring.
NCBI tax id
- NCBI tax id: 575186
- Matching level: species
strain history
- @ref: 21302
- history: <- A. Coleri Cihan, Ankara Univ., Turkey; E173a <- A. Coleri Cihan, Ankara Univ., Turkey; E173a
doi: 10.13145/bacdive23771.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Thermolongibacillus
- species: Thermolongibacillus kozakliensis
- full scientific name: Thermolongibacillus kozakliensis Cihan et al. 2014
@ref: 21302
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Thermolongibacillus
species: Thermolongibacillus kozakliensis
full scientific name: Thermolongibacillus kozakliensis Cihan et al. 2014
strain designation: E173a
type strain: yes
Morphology
cell morphology
- @ref: 31140
- gram stain: positive
- cell length: 3.0-8.0 µm
- cell width: 0.6-1 µm
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
- @ref: 21302
- name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
- growth: yes
- link: https://mediadive.dsmz.de/medium/220
- composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
21302 | positive | growth | 55 | thermophilic |
31140 | positive | growth | 40-70 | thermophilic |
31140 | positive | optimum | 60 | thermophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
31140 | positive | growth | 5.0-11.0 | alkaliphile |
31140 | positive | optimum | 9 |
Physiology and metabolism
oxygen tolerance
- @ref: 31140
- oxygen tolerance: aerobe
spore formation
- @ref: 31140
- spore formation: yes
halophily
- @ref: 31140
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 0.5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31140 | 17234 | glucose | + | carbon source |
31140 | 29864 | mannitol | + | carbon source |
31140 | 37684 | mannose | + | carbon source |
31140 | 33942 | ribose | + | carbon source |
31140 | 17992 | sucrose | + | carbon source |
31140 | 18222 | xylose | + | carbon source |
31140 | 17632 | nitrate | + | reduction |
Isolation, sampling and environmental information
isolation
- @ref: 21302
- sample type: soil beside a hotspring
- geographic location: Cappadocia area, Nevsehir, Kozakli Municipality thermal hot spring
- country: Turkey
- origin.country: TUR
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Environmental | #Terrestrial | #Soil |
#Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_169532.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_56247;97_82066;98_111148;99_169532&stattab=map
- Last taxonomy: Thermolongibacillus kozakliensis subclade
- 16S sequence: FJ430056
- Sequence Identity:
- Total samples: 45
- soil counts: 6
- aquatic counts: 16
- animal counts: 16
- plant counts: 7
Safety information
risk assessment
- @ref: 21302
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21302
- description: Thermolongibacillus kozakliensis strain E173a 16S ribosomal RNA gene, partial sequence
- accession: FJ430056
- length: 1400
- database: ena
- NCBI tax ID: 575186
GC content
@ref | GC-content | method |
---|---|---|
21302 | 44.8 | high performance liquid chromatography (HPLC) |
31140 | 44.8 |
External links
@ref: 21302
culture collection no.: DSM 24978, NCIMB 14849
straininfo link
- @ref: 87806
- straininfo: 403283
literature
- topic: Phylogeny
- Pubmed-ID: 24048871
- title: Thermolongibacillus altinsuensis gen. nov., sp. nov. and Thermolongibacillus kozakliensis sp. nov., aerobic, thermophilic, long bacilli isolated from hot springs.
- authors: Cihan AC, Koc M, Ozcan B, Tekin N, Cokmus C
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.053280-0
- year: 2013
- mesh: Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hot Springs/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Turkey, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Genetics
Reference
@id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
21302 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-24978 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24978) | |||
31140 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27468 | 28776041 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
87806 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID403283.1 |