Strain identifier

BacDive ID: 23771

Type strain: Yes

Species: Thermolongibacillus kozakliensis

Strain Designation: E173a

Strain history: <- A. Coleri Cihan, Ankara Univ., Turkey; E173a <- A. Coleri Cihan, Ankara Univ., Turkey; E173a

NCBI tax ID(s): 575186 (species)

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General

@ref: 21302

BacDive-ID: 23771

DSM-Number: 24978

keywords: 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped

description: Thermolongibacillus kozakliensis E173a is an aerobe, spore-forming, thermophilic bacterium that was isolated from soil beside a hotspring.

NCBI tax id

  • NCBI tax id: 575186
  • Matching level: species

strain history

  • @ref: 21302
  • history: <- A. Coleri Cihan, Ankara Univ., Turkey; E173a <- A. Coleri Cihan, Ankara Univ., Turkey; E173a

doi: 10.13145/bacdive23771.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Thermolongibacillus
  • species: Thermolongibacillus kozakliensis
  • full scientific name: Thermolongibacillus kozakliensis Cihan et al. 2014

@ref: 21302

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Thermolongibacillus

species: Thermolongibacillus kozakliensis

full scientific name: Thermolongibacillus kozakliensis Cihan et al. 2014

strain designation: E173a

type strain: yes

Morphology

cell morphology

  • @ref: 31140
  • gram stain: positive
  • cell length: 3.0-8.0 µm
  • cell width: 0.6-1 µm
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

  • @ref: 21302
  • name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/220
  • composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21302positivegrowth55thermophilic
31140positivegrowth40-70thermophilic
31140positiveoptimum60thermophilic

culture pH

@refabilitytypepHPH range
31140positivegrowth5.0-11.0alkaliphile
31140positiveoptimum9

Physiology and metabolism

oxygen tolerance

  • @ref: 31140
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31140
  • spore formation: yes

halophily

  • @ref: 31140
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 0.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3114017234glucose+carbon source
3114029864mannitol+carbon source
3114037684mannose+carbon source
3114033942ribose+carbon source
3114017992sucrose+carbon source
3114018222xylose+carbon source
3114017632nitrate+reduction

Isolation, sampling and environmental information

isolation

  • @ref: 21302
  • sample type: soil beside a hotspring
  • geographic location: Cappadocia area, Nevsehir, Kozakli Municipality thermal hot spring
  • country: Turkey
  • origin.country: TUR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Environmental#Terrestrial#Soil
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_169532.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_56247;97_82066;98_111148;99_169532&stattab=map
  • Last taxonomy: Thermolongibacillus kozakliensis subclade
  • 16S sequence: FJ430056
  • Sequence Identity:
  • Total samples: 45
  • soil counts: 6
  • aquatic counts: 16
  • animal counts: 16
  • plant counts: 7

Safety information

risk assessment

  • @ref: 21302
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21302
  • description: Thermolongibacillus kozakliensis strain E173a 16S ribosomal RNA gene, partial sequence
  • accession: FJ430056
  • length: 1400
  • database: ena
  • NCBI tax ID: 575186

GC content

@refGC-contentmethod
2130244.8high performance liquid chromatography (HPLC)
3114044.8

External links

@ref: 21302

culture collection no.: DSM 24978, NCIMB 14849

straininfo link

  • @ref: 87806
  • straininfo: 403283

literature

  • topic: Phylogeny
  • Pubmed-ID: 24048871
  • title: Thermolongibacillus altinsuensis gen. nov., sp. nov. and Thermolongibacillus kozakliensis sp. nov., aerobic, thermophilic, long bacilli isolated from hot springs.
  • authors: Cihan AC, Koc M, Ozcan B, Tekin N, Cokmus C
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.053280-0
  • year: 2013
  • mesh: Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hot Springs/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Turkey, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21302Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-24978Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24978)
31140Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172746828776041
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
87806Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403283.1