Strain identifier
BacDive ID: 23770
Type strain: 
Species: Thermolongibacillus altinsuensis
Strain Designation: E265
Strain history: <- A. Coleri Cihan, Ankara Univ., Turkey; E265
NCBI tax ID(s): 575256 (species)
version 8.1 (current version)
General
@ref: 21301
BacDive-ID: 23770
DSM-Number: 24979
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped
description: Thermolongibacillus altinsuensis E265 is an aerobe, spore-forming, thermophilic bacterium that was isolated from hot spring sediment.
NCBI tax id
- NCBI tax id: 575256
- Matching level: species
strain history
- @ref: 21301
- history: <- A. Coleri Cihan, Ankara Univ., Turkey; E265
doi: 10.13145/bacdive23770.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Thermolongibacillus
- species: Thermolongibacillus altinsuensis
- full scientific name: Thermolongibacillus altinsuensis Cihan et al. 2014
@ref: 21301
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Thermolongibacillus
species: Thermolongibacillus altinsuensis
full scientific name: Thermolongibacillus altinsuensis Cihan et al. 2014
strain designation: E265
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 31140 | positive | 3.5-8.0 µm | 0.7-1.1 µm | rod-shaped | yes | |
| 69480 | yes | 95.538 | ||||
| 69480 | positive | 100 |
Culture and growth conditions
culture medium
- @ref: 21301
- name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
- growth: yes
- link: https://mediadive.dsmz.de/medium/220
- composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 21301 | positive | growth | 55 | thermophilic | |
| 31140 | positive | growth | 40-70 | thermophilic | |
| 31140 | positive | optimum | 55 | thermophilic | |
| 69480 | thermophilic | 95.906 |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 31140 | positive | growth | 5.0-11.0 | alkaliphile |
| 31140 | positive | optimum | 8.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 31140
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 31140 | yes | |
| 69481 | yes | 100 |
| 69480 | yes | 100 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 31140 | NaCl | positive | growth | 0-5 % |
| 31140 | NaCl | positive | optimum | 3 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 31140 | 22599 | arabinose | + | carbon source |
| 31140 | 28260 | galactose | + | carbon source |
| 31140 | 17716 | lactose | + | carbon source |
| 31140 | 29864 | mannitol | + | carbon source |
| 31140 | 37684 | mannose | + | carbon source |
| 31140 | 16634 | raffinose | + | carbon source |
| 31140 | 30911 | sorbitol | + | carbon source |
| 31140 | 18222 | xylose | + | carbon source |
| 31140 | 17632 | nitrate | + | reduction |
Isolation, sampling and environmental information
isolation
- @ref: 21301
- sample type: hot spring sediment
- geographic location: Cappadocia area, Nevsehir, Kozakli, Altinsu hot spring (34° 43' E 38° 38' N)
- country: Turkey
- origin.country: TUR
- continent: Asia
- latitude: 38.6333
- longitude: 34.7167
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Sediment |
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_147671.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_56247;97_72670;98_97710;99_147671&stattab=map
- Last taxonomy: Thermolongibacillus altinsuensis subclade
- 16S sequence: FJ429590
- Sequence Identity:
- Total samples: 152
- soil counts: 75
- aquatic counts: 28
- animal counts: 34
- plant counts: 15
Safety information
risk assessment
- @ref: 21301
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21301
- description: Thermolongibacillus altinsuensis strain E265 16S ribosomal RNA gene, partial sequence
- accession: FJ429590
- length: 1432
- database: ena
- NCBI tax ID: 575256
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermolongibacillus altinsuensis DSM 24979 | GCA_004341205 | scaffold | ncbi | 575256 |
| 66792 | Thermolongibacillus altinsuensis strain DSM 24979 | 575256.3 | wgs | patric | 575256 |
| 66792 | Thermolongibacillus altinsuensis DSM 24979 | 2788499848 | draft | img | 575256 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 21301 | 43.5 | high performance liquid chromatography (HPLC) |
| 31140 | 43.5 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| flagellated | yes | 71.84 | no |
| gram-positive | yes | 88.287 | no |
| anaerobic | no | 95.14 | no |
| aerobic | yes | 77.65 | yes |
| halophile | no | 88.025 | no |
| spore-forming | yes | 94.187 | yes |
| thermophile | yes | 99.615 | yes |
| glucose-util | yes | 87.071 | no |
| motile | yes | 89.232 | yes |
| glucose-ferment | no | 85.305 | no |
External links
@ref: 21301
culture collection no.: DSM 24979, NCIMB 14850
straininfo link
- @ref: 87805
- straininfo: 397100
literature
- topic: Phylogeny
- Pubmed-ID: 24048871
- title: Thermolongibacillus altinsuensis gen. nov., sp. nov. and Thermolongibacillus kozakliensis sp. nov., aerobic, thermophilic, long bacilli isolated from hot springs.
- authors: Cihan AC, Koc M, Ozcan B, Tekin N, Cokmus C
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.053280-0
- year: 2013
- mesh: Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hot Springs/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Turkey, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Genetics
Reference
| @id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||||
| 21301 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-24979 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24979) | ||||
| 31140 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27468 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | ||||
| 87805 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID397100.1 |