Strain identifier
BacDive ID: 23764
Type strain:
Species: Tumebacillus flagellatus
Strain Designation: GST4, NFB
Strain history: <- Q. Wang, Guangxi Academy of Sciences, Nat. Eng. Res. Center, Nanning, China; GST4
NCBI tax ID(s): 1157490 (species)
General
@ref: 21187
BacDive-ID: 23764
DSM-Number: 25748
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped
description: Tumebacillus flagellatus GST4 is an aerobe, spore-forming, mesophilic bacterium that was isolated from wastewater of cassava starch factory.
NCBI tax id
- NCBI tax id: 1157490
- Matching level: species
strain history
- @ref: 21187
- history: <- Q. Wang, Guangxi Academy of Sciences, Nat. Eng. Res. Center, Nanning, China; GST4
doi: 10.13145/bacdive23764.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Alicyclobacillaceae
- genus: Tumebacillus
- species: Tumebacillus flagellatus
- full scientific name: Tumebacillus flagellatus Wang et al. 2013
@ref: 21187
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Alicyclobacillaceae
genus: Tumebacillus
species: Tumebacillus flagellatus
full scientific name: Tumebacillus flagellatus Wang et al. 2013
strain designation: GST4, NFB
type strain: yes
Morphology
cell morphology
- @ref: 30825
- gram stain: positive
- cell length: 3.65 µm
- cell width: 0.5 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 30825
- production: yes
Culture and growth conditions
culture medium
- @ref: 21187
- name: R2A MEDIUM (DSMZ Medium 830)
- growth: yes
- link: https://mediadive.dsmz.de/medium/830
- composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
21187 | positive | growth | 37 | mesophilic |
30825 | positive | growth | 20-42 | |
30825 | positive | optimum | 37 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
30825 | positive | growth | 4.5-8.5 | alkaliphile |
30825 | positive | optimum | 5.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 30825
- oxygen tolerance: aerobe
spore formation
- @ref: 30825
- spore formation: yes
compound production
@ref | compound |
---|---|
21187 | alpha amylase |
21187 | pullulanase |
halophily
- @ref: 30825
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-1.0 %
murein
- @ref: 21187
- murein short key: A31
- type: A1gamma m-Dpm-direct
observation
- @ref: 30825
- observation: aggregates in clumps
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30825 | 17057 | cellobiose | + | carbon source |
30825 | 28260 | galactose | + | carbon source |
30825 | 17234 | glucose | + | carbon source |
30825 | 17716 | lactose | + | carbon source |
30825 | 29864 | mannitol | + | carbon source |
30825 | 30911 | sorbitol | + | carbon source |
30825 | 27082 | trehalose | + | carbon source |
30825 | 18222 | xylose | + | carbon source |
enzymes
@ref | value | activity | ec |
---|---|---|---|
30825 | gelatinase | + | |
30825 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
- @ref: 21187
- sample type: wastewater of cassava starch factory
- geographic location: Guangxi Autonomous Region (22° 17' 43'' N 107° 68' 27'' E)
- country: China
- origin.country: CHN
- continent: Asia
- latitude: 22.2953
- longitude: 108.141
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Industrial | #Plant (Factory) |
#Engineered | #Waste | #Wastewater |
taxonmaps
- @ref: 69479
- File name: preview.99_108545.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1221;96_15361;97_18791;98_73719;99_108545&stattab=map
- Last taxonomy: Tumebacillus flagellatus subclade
- 16S sequence: JQ421297
- Sequence Identity:
- Total samples: 1163
- soil counts: 825
- aquatic counts: 133
- animal counts: 154
- plant counts: 51
Safety information
risk assessment
- @ref: 21187
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21187
- description: Tumebacillus flagellatus strain GST4 16S ribosomal RNA gene, partial sequence
- accession: JQ421297
- length: 1502
- database: ena
- NCBI tax ID: 1157490
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Tumebacillus flagellatus GST4 | GCA_000714935 | contig | ncbi | 1157490 |
66792 | Tumebacillus flagellatus GST4 | 2585427972 | draft | img | 1157490 |
GC content
@ref | GC-content | method |
---|---|---|
21187 | 53.7 | thermal denaturation, midpoint method (Tm) |
30825 | 53.7 |
External links
@ref: 21187
culture collection no.: DSM 25748, CGMCC 1.12170
straininfo link
- @ref: 87800
- straininfo: 407215
literature
- topic: Phylogeny
- Pubmed-ID: 23435245
- title: Tumebacillus flagellatus sp. nov., an alpha-amylase/pullulanase-producing bacterium isolated from cassava wastewater.
- authors: Wang Q, Xie N, Qin Y, Shen N, Zhu J, Mi H, Huang R
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.045351-0
- year: 2013
- mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Glycoside Hydrolases/*biosynthesis, Gram-Positive Rods/*classification/genetics/isolation & purification, Manihot, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysis, Waste Water/*microbiology, alpha-Amylases/*biosynthesis
- topic2: Genetics
Reference
@id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed | journal |
---|---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||||
21187 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25748 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25748) | ||||
30825 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27156 | 28776041 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||||
87800 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID407215.1 |