Strain identifier

BacDive ID: 23764

Type strain: Yes

Species: Tumebacillus flagellatus

Strain Designation: GST4, NFB

Strain history: <- Q. Wang, Guangxi Academy of Sciences, Nat. Eng. Res. Center, Nanning, China; GST4

NCBI tax ID(s): 1157490 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21187

BacDive-ID: 23764

DSM-Number: 25748

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Tumebacillus flagellatus GST4 is an aerobe, spore-forming, mesophilic bacterium that was isolated from wastewater of cassava starch factory.

NCBI tax id

  • NCBI tax id: 1157490
  • Matching level: species

strain history

  • @ref: 21187
  • history: <- Q. Wang, Guangxi Academy of Sciences, Nat. Eng. Res. Center, Nanning, China; GST4

doi: 10.13145/bacdive23764.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Alicyclobacillaceae
  • genus: Tumebacillus
  • species: Tumebacillus flagellatus
  • full scientific name: Tumebacillus flagellatus Wang et al. 2013

@ref: 21187

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Alicyclobacillaceae

genus: Tumebacillus

species: Tumebacillus flagellatus

full scientific name: Tumebacillus flagellatus Wang et al. 2013

strain designation: GST4, NFB

type strain: yes

Morphology

cell morphology

  • @ref: 30825
  • gram stain: positive
  • cell length: 3.65 µm
  • cell width: 0.5 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 30825
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 21187
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21187positivegrowth37mesophilic
30825positivegrowth20-42
30825positiveoptimum37mesophilic

culture pH

@refabilitytypepHPH range
30825positivegrowth4.5-8.5alkaliphile
30825positiveoptimum5.5

Physiology and metabolism

oxygen tolerance

  • @ref: 30825
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30825
  • spore formation: yes

compound production

@refcompound
21187alpha amylase
21187pullulanase

halophily

  • @ref: 30825
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-1.0 %

murein

  • @ref: 21187
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 30825
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3082517057cellobiose+carbon source
3082528260galactose+carbon source
3082517234glucose+carbon source
3082517716lactose+carbon source
3082529864mannitol+carbon source
3082530911sorbitol+carbon source
3082527082trehalose+carbon source
3082518222xylose+carbon source

enzymes

@refvalueactivityec
30825gelatinase+
30825cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

  • @ref: 21187
  • sample type: wastewater of cassava starch factory
  • geographic location: Guangxi Autonomous Region (22° 17' 43'' N 107° 68' 27'' E)
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 22.2953
  • longitude: 108.141

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Plant (Factory)
#Engineered#Waste#Wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_108545.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1221;96_15361;97_18791;98_73719;99_108545&stattab=map
  • Last taxonomy: Tumebacillus flagellatus subclade
  • 16S sequence: JQ421297
  • Sequence Identity:
  • Total samples: 1163
  • soil counts: 825
  • aquatic counts: 133
  • animal counts: 154
  • plant counts: 51

Safety information

risk assessment

  • @ref: 21187
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21187
  • description: Tumebacillus flagellatus strain GST4 16S ribosomal RNA gene, partial sequence
  • accession: JQ421297
  • length: 1502
  • database: ena
  • NCBI tax ID: 1157490

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Tumebacillus flagellatus GST4GCA_000714935contigncbi1157490
66792Tumebacillus flagellatus GST42585427972draftimg1157490

GC content

@refGC-contentmethod
2118753.7thermal denaturation, midpoint method (Tm)
3082553.7

External links

@ref: 21187

culture collection no.: DSM 25748, CGMCC 1.12170

straininfo link

  • @ref: 87800
  • straininfo: 407215

literature

  • topic: Phylogeny
  • Pubmed-ID: 23435245
  • title: Tumebacillus flagellatus sp. nov., an alpha-amylase/pullulanase-producing bacterium isolated from cassava wastewater.
  • authors: Wang Q, Xie N, Qin Y, Shen N, Zhu J, Mi H, Huang R
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.045351-0
  • year: 2013
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Glycoside Hydrolases/*biosynthesis, Gram-Positive Rods/*classification/genetics/isolation & purification, Manihot, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysis, Waste Water/*microbiology, alpha-Amylases/*biosynthesis
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmedjournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21187Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-25748Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25748)
30825Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172715628776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa102533211880Nucleic Acids Res. 49: D498-D508 2020
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
87800Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407215.1