Strain identifier

BacDive ID: 23712

Type strain: Yes

Species: Oikeobacillus pervagus

Strain Designation: 8-4-E12

Strain history: <- U. Lechner, Martin-Luther-University Halle-Wittenberg, Inst. of Biology/Microbiology, Germany; 8-4-E12 <- K. Worm

NCBI tax ID(s): 1325931 (species)

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General

@ref: 20440

BacDive-ID: 23712

DSM-Number: 23947

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-positive, motile

description: Oikeobacillus pervagus 8-4-E12 is a mesophilic, Gram-positive, motile bacterium that was isolated from biowaste composting reactor.

NCBI tax id

  • NCBI tax id: 1325931
  • Matching level: species

strain history

  • @ref: 20440
  • history: <- U. Lechner, Martin-Luther-University Halle-Wittenberg, Inst. of Biology/Microbiology, Germany; 8-4-E12 <- K. Worm

doi: 10.13145/bacdive23712.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Oikeobacillus
  • species: Oikeobacillus pervagus
  • full scientific name: Oikeobacillus pervagus (Kosowski et al. 2014) Narsing Rao et al. 2023

  • synonyms

    • @ref: 20215
    • synonym: Bacillus pervagus

@ref: 20440

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus pervagus

full scientific name: Bacillus pervagus Kosowski et al. 2014

strain designation: 8-4-E12

type strain: yes

Morphology

cell morphology

  • @ref: 31171
  • gram stain: positive
  • cell length: 2.0-3.4 µm
  • cell width: 0.8-1.3 µm
  • motility: yes

Culture and growth conditions

culture medium

  • @ref: 20440
  • name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/220
  • composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
20440positivegrowth37mesophilic
31171positivegrowth20-50
31171positiveoptimum45thermophilic

culture pH

@refabilitytypepHPH range
31171positivegrowth05-09alkaliphile
31171positiveoptimum8

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
31171NaClpositivegrowth02-07 %
31171NaClpositiveoptimum2 %

observation

  • @ref: 31171
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
31171370543-hydroxybutyrate+carbon source
3117117754glycerol+carbon source
3117125115malate+carbon source
3117115361pyruvate+carbon source
3117117632nitrate+reduction

enzymes

  • @ref: 31171
  • value: cytochrome oxidase
  • activity: +
  • ec: 1.9.3.1

Isolation, sampling and environmental information

isolation

  • @ref: 20440
  • sample type: biowaste composting reactor
  • geographic location: federal state of Saxony, Bad Lausick
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Composting
#Engineered#Bioreactor

taxonmaps

  • @ref: 69479
  • File name: preview.99_37145.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1672;97_21773;98_27333;99_37145&stattab=map
  • Last taxonomy: Bacillus pervagus subclade
  • 16S sequence: HF952773
  • Sequence Identity:
  • Total samples: 1285
  • soil counts: 523
  • aquatic counts: 129
  • animal counts: 530
  • plant counts: 103

Safety information

risk assessment

  • @ref: 20440
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20440
  • description: Bacillus pervagus partial 16S rRNA gene, strain 8-4-E12
  • accession: HF952773
  • length: 1463
  • database: ena
  • NCBI tax ID: 1325931

Genome sequences

  • @ref: 66792
  • description: Bacillus pervagus DSM 23947
  • accession: 2926534574
  • assembly level: draft
  • database: img
  • NCBI tax ID: 1325931

GC content

@refGC-contentmethod
2044036.1high performance liquid chromatography (HPLC)
3117136.1

External links

@ref: 20440

culture collection no.: DSM 23947, LMG 27601

straininfo link

  • @ref: 87758
  • straininfo: 388542

literature

  • topic: Phylogeny
  • Pubmed-ID: 24021730
  • title: Bacillus pervagus sp. nov. and Bacillus andreesenii sp. nov., isolated from a composting reactor.
  • authors: Kosowski K, Schmidt M, Pukall R, Hause G, Kampfer P, Lechner U
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.054833-0
  • year: 2013
  • mesh: Bacillus/*classification/genetics/isolation & purification, Base Composition, Bioreactors/*microbiology, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phosphatidylethanolamines/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Refuse Disposal, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20440Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-23947Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23947)
31171Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172749928776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
87758Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID388542.1