Strain identifier

BacDive ID: 2366

Type strain: Yes

Species: Cellulomonas iranensis

Strain Designation: O

Strain history: CIP <- 2002, ATCC <- M. Shahamat <- F. Malekzadeh

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General

@ref: 5539

BacDive-ID: 2366

DSM-Number: 14785

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive

description: Cellulomonas iranensis O is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from forest soil.

NCBI tax id

NCBI tax idMatching level
76862species
1223509strain

strain history

@refhistory
5539<- IFO <- ATCC <- M. Shahamat; O
67770CIP 107678 <-- ATCC 700643 <-- M. Shahamat and F. Malekzadeh strain O.
120280CIP <- 2002, ATCC <- M. Shahamat <- F. Malekzadeh

doi: 10.13145/bacdive2366.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Cellulomonas
  • species: Cellulomonas iranensis
  • full scientific name: Cellulomonas iranensis Elberson et al. 2000

@ref: 5539

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Cellulomonas

species: Cellulomonas iranensis

full scientific name: Cellulomonas iranensis Elberson et al. 2000

strain designation: O

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
120280positiverod-shapedyes

colony morphology

  • @ref: 120280

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5539TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
39921MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120280CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5539positivegrowth28mesophilic
39921positivegrowth30mesophilic
67770positivegrowth28mesophilic
120280positivegrowth15-45
120280nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120280
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.908

halophily

@refsaltgrowthtested relationconcentration
120280NaClpositivegrowth0-6 %
120280NaClnogrowth8 %
120280NaClnogrowth10 %

murein

  • @ref: 5539
  • murein short key: A21.04
  • type: A4ß L-Orn-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside+builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose+builds acid from30849
68371erythritol-builds acid from17113
120280citrate-carbon source16947
120280esculin+hydrolysis4853
120280hippurate+hydrolysis606565
120280nitrate-reduction17632
120280nitrite-reduction16301
120280nitrate+respiration17632

antibiotic resistance

  • @ref: 120280
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 120280
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12028015688acetoin-
12028017234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
120280oxidase+
120280beta-galactosidase+3.2.1.23
120280alcohol dehydrogenase-1.1.1.1
120280gelatinase+/-
120280amylase+
120280DNase+
120280caseinase+3.4.21.50
120280catalase+1.11.1.6
120280cellulase-3.2.1.4
120280tween esterase+
120280gamma-glutamyltransferase-2.3.2.2
120280lecithinase-
120280lipase-
120280lysine decarboxylase-4.1.1.18
120280ornithine decarboxylase-4.1.1.17
120280phenylalanine ammonia-lyase-4.3.1.24
120280tryptophan deaminase-
120280urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120280--++-+-----+++-+++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120280+/--+/-+-+--+++++/-----+/------+++++/-+/--++/----++-+/-+/----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120280++++---+-++++--++-+-+++--------+-+-+---------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5539forest soilIran, near the Caspian SeaIranIRNAsia
67770Forest humus soilRamsarIranIRNAsia
120280Environment, Humus soil, Ramsar forestIranIRNAsia1992

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_3003.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_432;97_1685;98_2313;99_3003&stattab=map
  • Last taxonomy: Cellulomonas
  • 16S sequence: AB695190
  • Sequence Identity:
  • Total samples: 1253
  • soil counts: 334
  • aquatic counts: 224
  • animal counts: 635
  • plant counts: 60

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
55391Risk group (German classification)
1202801Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5539Cellulomonas iranensis strain O 16S ribosomal RNA gene, partial sequenceAF0647021378ena76862
67770Cellulomonas iranensis gene for 16S rRNA, partial sequence, strain: JCM 18110AB6951901459ena76862

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulomonas iranensis NBRC 101100 = JCM 18110GCA_001315225contigncbi1223509
66792Cellulomonas iranensis NBRC 101100 = JCM 181101223509.4wgspatric1223509
66792Cellulomonas iranensis NBRC 101100 = JCM 181101223509.5wgspatric1223509
66792Cellulomonas iranensis DSM 147852927609659draftimg76862
66792Cellulomonas iranensis NBRC 1011002731957583draftimg1223509
66792Cellulomonas iranensis JCM 181102734481977draftimg1223509
67770Cellulomonas iranensis NBRC 101100 = JCM 18110 NBRC 101100GCA_001552375contigncbi1223509

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes72no
motileno83.949no
gram-positiveyes64.706no
anaerobicno94.956no
aerobicyes61.747no
halophileno73.006no
spore-formingno94.052no
thermophileno96.837no
glucose-utilyes85.218no
flagellatedno92.71no
glucose-fermentyes69.366no

External links

@ref: 5539

culture collection no.: DSM 14785, ATCC 700643, IFO 16734, NBRC 16734, JCM 18110, CIP 107678, JCM 12410, KCTC 9983, NBRC 101100

straininfo link

  • @ref: 71921
  • straininfo: 45441

literature

  • topic: Phylogeny
  • Pubmed-ID: 32038002
  • title: Cellulomonas shaoxiangyii sp. nov., isolated from faeces of Tibetan antelope (Pantholops hodgsonii) on the Qinghai-Tibet Plateau.
  • authors: Tian Z, Lu S, Jin D, Yang J, Pu J, Lai XH, Ren ZH, Wu XM, Li J, Wang S, Xu J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003939
  • year: 2020
  • mesh: Animals, Antelopes/*microbiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cellulomonas/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Nucleic Acid Hybridization, Ornithine/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tibet, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitle
5539Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14785)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14785
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39921Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5140
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71921Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45441.1StrainInfo: A central database for resolving microbial strain identifiers
120280Curators of the CIPCollection of Institut Pasteur (CIP 107678)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107678