Strain identifier

BacDive ID: 2359

Type strain: Yes

Species: Cellulomonas humilata

Strain Designation: PSU-16S

Strain history: CIP <- 1995, ATCC <- L.E. Casida: strain PSU-16S

NCBI tax ID(s): 144055 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 5324

BacDive-ID: 2359

DSM-Number: 14368

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive

description: Cellulomonas humilata PSU-16S is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 144055
  • Matching level: species

strain history

@refhistory
5324<- CCUG; CCUG 35596 <- NRRL <- Casida, L. E., Jr
67770CIP 104614 <-- ATCC 25174 <-- L. E. Casida, Jr. PSU-16S.
119987CIP <- 1995, ATCC <- L.E. Casida: strain PSU-16S

doi: 10.13145/bacdive2359.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Cellulomonas
  • species: Cellulomonas humilata
  • full scientific name: Cellulomonas humilata corrig. (Gledhill and Casida 1969) Collins and Pascual 2000

  • synonyms

    @refsynonym
    20215Actinomyces humiferus
    20215Cellulomonas humiferus

@ref: 5324

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Cellulomonas

species: Cellulomonas humilata

full scientific name: Cellulomonas humilata (Gledhill and Casida 1969) Collins and Pascual 2000

strain designation: PSU-16S

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
119987positiverod-shapedno

colony morphology

  • @ref: 119987

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5324COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
5324BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
38511MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
119987CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5324positivegrowth30mesophilic
38511positivegrowth30mesophilic
52846positivegrowth30-37mesophilic
67770positivegrowth30mesophilic
119987positivegrowth25-37mesophilic
119987nogrowth10psychrophilic
119987nogrowth41thermophilic
119987nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5324microaerophile
5324aerobe
52846aerobe
52846anaerobe
52846microaerophile
119987facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.641

halophily

  • @ref: 119987
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11998716947citrate-carbon source
1199874853esculin+hydrolysis
119987606565hippurate+hydrolysis
11998717632nitrate-reduction
11998716301nitrite-reduction
11998717632nitrate+respiration

metabolite production

  • @ref: 119987
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11998715688acetoin-
11998717234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
119987oxidase-
119987beta-galactosidase+3.2.1.23
119987alcohol dehydrogenase-1.1.1.1
119987gelatinase-
119987amylase+
119987DNase-
119987caseinase+3.4.21.50
119987catalase-1.11.1.6
119987tween esterase-
119987gamma-glutamyltransferase-2.3.2.2
119987lecithinase-
119987lipase-
119987lysine decarboxylase-4.1.1.18
119987ornithine decarboxylase-4.1.1.17
119987phenylalanine ammonia-lyase-4.3.1.24
119987tryptophan deaminase-
119987urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119987-+++-++--++++--+----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119987+++-+-+++++++--++-+-++-+-+-----+-+-+------------------+----+---------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5324soilUSAUSANorth America
52846Soil
67770SoilUSAUSANorth America
119987Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_3436.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_432;97_1685;98_2637;99_3436&stattab=map
  • Last taxonomy: Cellulomonas
  • 16S sequence: X82449
  • Sequence Identity:
  • Total samples: 5905
  • soil counts: 3471
  • aquatic counts: 607
  • animal counts: 1257
  • plant counts: 570

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53241Risk group (German classification)
1199871Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5324
  • description: A.humiferus 16S rRNA gene
  • accession: X82449
  • length: 1402
  • database: ena
  • NCBI tax ID: 144055

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulomonas humilata strain ATCC 25174144055.3wgspatric144055
67770Cellulomonas humilata ATCC 25174GCA_013359775contigncbi144055

GC content

  • @ref: 67770
  • GC-content: 73

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno74no
motileyes74.67no
gram-positiveyes92.688no
anaerobicno98.811no
halophileno95.162no
spore-formingno86.47no
thermophileno98.914yes
glucose-utilyes86.282no
aerobicyes88.889no
flagellatedno93.05no
glucose-fermentno67.008no

External links

@ref: 5324

culture collection no.: DSM 14368, ATCC 25174, CCUG 35596, NRRL B-3752, JCM 11945, CIP 104614, NCIMB 10802, PCM 2416

straininfo link

  • @ref: 71914
  • straininfo: 45740

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15023971Cellulomonas xylanilytica sp. nov., a cellulolytic and xylanolytic bacterium isolated from a decayed elm tree.Rivas R, Trujillo ME, Mateos PF, Martinez-Molina E, Velazquez EInt J Syst Evol Microbiol10.1099/ijs.0.02866-02004Base Composition, Cellulomonas/*classification/genetics/growth & development/isolation & purification, Cellulose/metabolism, DNA, Bacterial/chemistry/genetics, Molecular Sequence Data, Phylogeny, Ulmus/*microbiology, Xylans/metabolismMetabolism
Phylogeny16014505Cellulomonas terrae sp. nov., a cellulolytic and xylanolytic bacterium isolated from soil.An DS, Im WT, Yang HC, Kang MS, Kim KK, Jin L, Kim MK, Lee STInt J Syst Evol Microbiol10.1099/ijs.0.63696-02005Bacterial Typing Techniques, Cellulomonas/*classification/genetics/isolation & purification/physiology, Cellulose/*metabolism, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity, Xylans/*metabolismMetabolism
Phylogeny30702420Cellulomonas rhizosphaerae sp. nov., a novel actinomycete isolated from soil.Tian Y, Han C, Hu J, Zhao J, Zhang C, Guo X, Wang X, Xiang WInt J Syst Evol Microbiol10.1099/ijsem.0.0032582019Bacterial Typing Techniques, Base Composition, Cellulomonas/*classification/isolation & purification, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistryTranscriptome
Phylogeny31218500Cellulomonas aurantiaca sp. nov., isolated from a soil sample from a tangerine field.Kim SK, Kook M, Yan ZF, Trinh H, Zheng SD, Yang JE, Park SY, Yi THAntonie Van Leeuwenhoek10.1007/s10482-019-01288-22019Cellulomonas/*classification/genetics/*isolation & purification, *Citrus, Genome, Bacterial, Genomics/methods, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Soil, *Soil MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitle
5324Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14368)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14368
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38511Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16542
52846Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 35596)https://www.ccug.se/strain?id=35596
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71914Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45740.1StrainInfo: A central database for resolving microbial strain identifiers
119987Curators of the CIPCollection of Institut Pasteur (CIP 104614)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104614