Strain identifier

BacDive ID: 2356

Type strain: Yes

Species: Cellulomonas gelida

Strain Designation: 126, NRS-126

Strain history: CIP <- 1986, DSM <- ATCC <- N.R. Smith: strain NRS-126

NCBI tax ID(s): 1712 (species)

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General

@ref: 8536

BacDive-ID: 2356

DSM-Number: 20111

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive

description: Cellulomonas gelida 126 is a spore-forming, mesophilic, Gram-positive bacterium of the family Cellulomonadaceae.

NCBI tax id

  • NCBI tax id: 1712
  • Matching level: species

strain history

@refhistory
8536<- ATCC <- N.R. Smith, 126
67770K. Suzuki CNF 198 <-- AJ 1567 <-- ATCC 488 <-- N. R. Smith NRS 126.
119564CIP <- 1986, DSM <- ATCC <- N.R. Smith: strain NRS-126

doi: 10.13145/bacdive2356.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Cellulomonas
  • species: Cellulomonas gelida
  • full scientific name: Cellulomonas gelida (Kellerman et al. 1913) Bergey et al. 1923 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus gelidus

@ref: 8536

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Cellulomonas

species: Cellulomonas gelida

full scientific name: Cellulomonas gelida (Kellerman et al. 1913) Bergey et al. 1923

strain designation: 126, NRS-126

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

@refcolony colorincubation periodmedium used
18417Light ivory (1015)10-14 daysISP 2
18417Ivory (1014)10-14 daysISP 3
18417Colorless10-14 daysISP 4
18417Colorless10-14 daysISP 5
18417Colorless10-14 daysISP 6
18417Colorless10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18417noISP 2
18417noISP 3
18417noISP 4
18417noISP 5
18417noISP 6
18417noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18417ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18417ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18417ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18417ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18417ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18417ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
8536CORYNEBACTERIUM AGAR (DSMZ Medium 53)yesName: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/53
35148MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
119564CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
18417positiveoptimum28mesophilic
8536positivegrowth30mesophilic
35148positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes95
69480no99.752

halophily

  • @ref: 18417
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

murein

  • @ref: 8536
  • murein short key: A21.05
  • type: A4ß L-Orn-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1841717234glucose-
1841722599arabinose-
1841718222xylose+
1841717268myo-inositol+
1841729864mannitol-
1841726546rhamnose+
1841762968cellulose+
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose+fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol+fermentation
6837917306maltose+fermentation
6837917716lactose+fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18417-++-+++++++++-++++-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18417+++-+++++++++-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18417+--------+-

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
35148Storrs, CTUSAUSANorth America
67770Storrs, CTUSAUSANorth AmericaSoil
119564Storrs, ConnecticutUnited States of AmericaUSANorth AmericaEnvironment, Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_8197.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_432;97_4150;98_5311;99_8197&stattab=map
  • Last taxonomy: Cellulomonas
  • 16S sequence: X79461
  • Sequence Identity:
  • Total samples: 236
  • soil counts: 127
  • aquatic counts: 39
  • animal counts: 57
  • plant counts: 13

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
184171
85361Risk group (German classification)
1195641Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218C.gelida 16S rRNA geneX838001500ena1712
20218C.gelda NCIMB 8076 16S rDNAX794611374ena1712

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulomonas gelida JCM 1490GCA_014647015scaffoldncbi1712
66792Cellulomonas gelida strain JCM 14901712.4wgspatric1712
66792Cellulomonas gelida strain NBRC 37481712.3wgspatric1712
67770Cellulomonas gelida NBRC 3748GCA_006538725contigncbi1712

GC content

@refGC-contentmethod
853672.4
6777072.4thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes95no
flagellatedno90.027no
gram-positiveyes90.6no
anaerobicno98.617no
aerobicyes78.422no
halophileno94.998no
spore-formingno86.685no
thermophileno99.043yes
glucose-utilyes75.076yes
motileyes77.99no
glucose-fermentno53.925yes

External links

@ref: 8536

culture collection no.: DSM 20111, ATCC 488, NCIB 8076, JCM 1490, BCRC 11370, CFBP 4257, CGMCC 1.1915, CIP 102221, IAM 12110, IFO 3748, IMET 11078, IMSNU 20370, KCCM 40762, KCTC 1439, KCTC 3231, LMG 16697, NBRC 3748, NCIMB 8076, IFO 3478

straininfo link

  • @ref: 71911
  • straininfo: 8214

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism1459948Cellobiose chemotaxis by the cellulolytic bacterium Cellulomonas gelida.Hsing W, Canale-Parola EJ Bacteriol10.1128/jb.174.24.7996-8002.19921992Actinomycetales/*metabolism/ultrastructure, Binding, Competitive, Cell Wall/metabolism, Cellobiose/*metabolism, Cellulose/metabolism, Chemotactic Factors/*metabolism, Galactose/metabolism, Glucose/metabolism, Glucose Oxidase/metabolism, Microscopy, Electron, Plants/metabolism, Polysaccharides/metabolism, Xylans/metabolism
Phylogeny32877326Cellulomonas citrea sp. nov., isolated from paddy soil.Lee HJ, Kim SY, Whang KSInt J Syst Evol Microbiol10.1099/ijsem.0.0044092020Bacterial Typing Techniques, Base Composition, Cellulomonas/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Oryza, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny35100105Cellulomonas fulva sp. nov., isolated from oil-contaminated soil.Dahal RH, Kim J, Kim DU, Dong K, Hong Y, Chaudhary DKInt J Syst Evol Microbiol10.1099/ijsem.0.0052092022Bacterial Typing Techniques, Base Composition, *Cellulomonas/classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Petroleum Pollution, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil PollutantsTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8536Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20111)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20111
18417Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20111.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35148Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/13884
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71911Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8214.1StrainInfo: A central database for resolving microbial strain identifiers
119564Curators of the CIPCollection of Institut Pasteur (CIP 102221)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102221