Strain identifier

BacDive ID: 2354

Type strain: Yes

Species: Cellulomonas flavigena

Strain Designation: 134

Culture col. no.: DSM 20109, ATCC 482, CCM 1926, NCIB 8073, CCUG 28996

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8534

BacDive-ID: 2354

DSM-Number: 20109

keywords: rod-shaped, gram-positive, mesophilic, aerobe, Bacteria, 16S sequence, genome sequence

description: Cellulomonas flavigena 134 is an aerobe, mesophilic, gram-positive bacterium of the family Cellulomonadaceae.

strain history: <- ATCC <- N.R. Smith, 134

doi: 10.13145/bacdive2354.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Cellulomonas
  • species: Cellulomonas flavigena
  • full scientific name: Cellulomonas flavigena (Kellerman and McBeth 1912) Bergey et al. 1923 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus flavigena

@ref: 8534

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Cellulomonas

species: Cellulomonas flavigena

full scientific name: Cellulomonas flavigena (Kellerman and McBeth 1912) Bergey et al. 1923 emend. Nouioui et al. 2018

strain designation: 134

type strain: yes

Morphology

cell morphology

  • @ref: 43361
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium usedcolony size
18620Ivory (1014)10-14 daysISP 2
18620Zinc yellow (1018)10-14 daysISP 3
18620Daffodil yellow (1007)10-14 daysISP 4
18620Zinc yellow (1018)10-14 daysISP 5
18620Colorless10-14 daysISP 6
18620Colorless10-14 daysISP 7
43361yellowyeast extract-glucose agar5 mm

multicellular morphology

@refforms multicellular complexmedium name
18620noISP 2
18620noISP 3
18620noISP 4
18620noISP 5
18620noISP 6
18620noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18620ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18620ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18620ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18620ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18620ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18620ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
8534CORYNEBACTERIUM AGAR (DSMZ Medium 53)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium53.pdf
37348MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
43361yeast extract-glucose agaryes
43361yeast glucose agaryespeptone 1%, yeast extract 0.5%, glucose 0.5%, agar 1%

culture temp

@refgrowthtypetemperaturerange
18620positiveoptimum28mesophilic
8534positivegrowth30mesophilic
37348positivegrowth30mesophilic
43361positiveoptimum30mesophilic
49475positivegrowth26-42

culture pH

  • @ref: 43361
  • ability: positive
  • type: optimum
  • pH: 7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43361aerobe
49475aerobe

halophily

  • @ref: 18620
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

murein

  • @ref: 8534
  • murein short key: A21.04
  • type: A4ß L-Orn-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4336116004(R)-lactate-assimilation
4336116651(S)-lactate-assimilation
4336130089acetate+assimilation
4336117234glucose+assimilation
4336117632nitrate+reduction
4336116634raffinose-assimilation
4336133942ribose+assimilation

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    49475C14:015.614
    49475C16:014.716
    49475C14:0 ISO7.813.618
    49475C15:0 ANTEISO45.714.711
    49475C15:0 ISO4.614.621
    49475C15:1 ANTEISO A8.414.526
    49475C16:0 iso3.215.626
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18620--+-+++++++-+-+++--

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18620--+-+++++++-+-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18620+----------

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
186201Hazard group
85341Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: C.flavigena 16S rRNA gene
  • accession: X83799
  • length: 1500
  • database: ena
  • NCBI tax ID: 446466

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulomonas flavigena DSM 20109GCA_000092865completencbi446466
66792Cellulomonas flavigena DSM 20109446466.7completepatric446466
66792Cellulomonas flavigena 134, DSM 20109646564520completeimg446466

GC content

@refGC-contentmethod
853472.7
4336172.7-74.8thermal denaturation, midpoint method (Tm)

External links

@ref: 8534

culture collection no.: DSM 20109, ATCC 482, CCM 1926, NCIB 8073, CCUG 28996

straininfo link

@refpassport
20218http://www.straininfo.net/strains/33676
20218http://www.straininfo.net/strains/33670
20218http://www.straininfo.net/strains/33672
20218http://www.straininfo.net/strains/93827

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8534Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20109)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20109
18620Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20109.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37348Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11277
43361Erko Stackenbrandt, Otto Kandler10.1099/00207713-29-4-273Taxonomy of the Genus Cellulomonas, Based on Phenotypic Characters and Deoxyribonucleic Acid-Deoxyribonucleic Acid Homology, and Proposal of Seven Neotype StrainsIJSEM 29: 273-282 1979
49475Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 28996)https://www.ccug.se/strain?id=28996
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)