Strain identifier

BacDive ID: 2351

Type strain: Yes

Species: Cellulomonas cellasea

Strain Designation: 124, NRS-124

Strain history: CIP <- 1986, DSM <- ATCC <- NCIB <- N.R. Smith: strain NRS-124

NCBI tax ID(s): 1408250 (strain), 43670 (species)

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General

@ref: 8543

BacDive-ID: 2351

DSM-Number: 20118

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive

description: Cellulomonas cellasea 124 is an obligate aerobe, mesophilic, Gram-positive bacterium of the family Cellulomonadaceae.

NCBI tax id

NCBI tax idMatching level
43670species
1408250strain

strain history

@refhistory
8543<- ATCC <- NCIB <- N.R . Smith, 124
67770DSM 20118 <-- ATCC 487 <-- N. R. Smith NRS 124.
117234CIP <- 1986, DSM <- ATCC <- NCIB <- N.R. Smith: strain NRS-124

doi: 10.13145/bacdive2351.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Cellulomonas
  • species: Cellulomonas cellasea
  • full scientific name: Cellulomonas cellasea (Kellerman et al. 1913) Bergey et al. 1923 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus cellaseus

@ref: 8543

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Cellulomonas

species: Cellulomonas cellasea

full scientific name: Cellulomonas cellasea (Kellerman et al. 1913) Bergey et al. 1923 emend. Nouioui et al. 2018

strain designation: 124, NRS-124

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
117234positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
18621Oyster white (1013)10-14 daysISP 2
18621Sulfur white (1016)10-14 daysISP 3
18621Sulfur white (1016)10-14 daysISP 4
18621Zinc yellow (1018)10-14 daysISP 5
18621Colorless10-14 daysISP 6
18621Colorless10-14 daysISP 7
117234

multicellular morphology

@refforms multicellular complexmedium name
18621noISP 2
18621noISP 3
18621noISP 4
18621noISP 5
18621noISP 6
18621noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18621ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18621ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18621ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18621ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18621ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18621ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
8543CORYNEBACTERIUM AGAR (DSMZ Medium 53)yesName: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/53
40379MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
117234CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
18621positiveoptimum28mesophilic
8543positivegrowth30mesophilic
40379positivegrowth30mesophilic
67770positivegrowth30mesophilic
117234positivegrowth25-30mesophilic
117234nogrowth10psychrophilic
117234nogrowth15psychrophilic
117234nogrowth37mesophilic
117234nogrowth41thermophilic
117234nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 117234
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.801

halophily

@refsaltgrowthtested relationconcentration
18621NaClpositivemaximum2.5 %
117234NaClpositivegrowth0-2 %
117234NaClnogrowth4 %
117234NaClnogrowth6 %
117234NaClnogrowth8 %
117234NaClnogrowth10 %

murein

  • @ref: 8543
  • murein short key: A21.05
  • type: A4ß L-Orn-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1862162968cellulose-
1862116634raffinose+
1862126546rhamnose+
1862128757fructose-
1862129864mannitol-
1862117268myo-inositol+
1862118222xylose+
1862117992sucrose+
1862122599arabinose+
1862117234glucose+
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose+builds acid from
6837115443inulin+builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
11723416947citrate-carbon source
1172344853esculin+hydrolysis
117234606565hippurate-hydrolysis
11723417632nitrate+reduction
11723416301nitrite-reduction
11723417632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose+fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol+fermentation
6837917306maltose+fermentation
6837917716lactose+fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
11723435581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
11723415688acetoin+
11723417234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
117234oxidase-
117234beta-galactosidase+3.2.1.23
117234alcohol dehydrogenase-1.1.1.1
117234gelatinase-
117234amylase-
117234DNase-
117234caseinase-3.4.21.50
117234catalase+1.11.1.6
117234cellulase-3.2.1.4
117234tween esterase-
117234gamma-glutamyltransferase-2.3.2.2
117234lecithinase-
117234lipase-
117234lysine decarboxylase-4.1.1.18
117234ornithine decarboxylase-4.1.1.17
117234phenylalanine ammonia-lyase-4.3.1.24
117234tryptophan deaminase-
117234urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18621-++-+++--++++-++++-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18621+++-+++--++++-+++--
117234+++-+----+++-++++---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18621+--------+-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117234---+++---++++----+/----++/-+++++--++++/-+++-++------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117234+++----+-++----++++-++++-+-------+++--------------------------------------------------+--++--------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
40379UtahUSAUSANorth America
67770UTUSAUSANorth AmericaSoil
117234UtahUnited States of AmericaUSANorth AmericaEnvironment, Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
186211Hazard group
85431Risk group (German classification)
1172341Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218C.cellasea DSM 20118 16S rDNAX794591486ena1408250
20218C.cellasea 16S rRNA geneX838041453ena1408250

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulomonas cellasea DSM 201181408250.3wgspatric1408250
66792Cellulomonas cellasea strain NBRC 375343670.3wgspatric43670
66792Cellulomonas cellasea DSM 201182630968797draftimg1408250
67770Cellulomonas cellasea DSM 20118GCA_000767135contigncbi1408250
67770Cellulomonas cellasea NBRC 3753GCA_006538745contigncbi43670

GC content

@refGC-contentmethod
854374.8
6777074.6genome sequence analysis
6777075thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes77no
motileyes60.084no
gram-positiveyes88.48no
anaerobicno95.912no
halophileno82.321no
spore-formingno83.064no
thermophileno98.643yes
glucose-utilyes90.419yes
aerobicyes83.661no
flagellatedno93.901no
glucose-fermentno72.77no

External links

@ref: 8543

culture collection no.: DSM 20118, ATCC 487, CCM 1925, NCIB 8078, JCM 9967, BCRC 14865, CFBP 4260, CIP 102222, IFO 3753, IFO 3774, KCCM 12154, KCTC 3410, LMG 16323, NBRC 3753, NBRC 3774, NCCB 29015, NCIMB 8078, VKM Ac-1136

straininfo link

  • @ref: 71906
  • straininfo: 13961

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16014506Cellulomonas bogoriensis sp. nov., an alkaliphilic cellulomonad.Jones BE, Grant WD, Duckworth AW, Schumann P, Weiss N, Stackebrandt EInt J Syst Evol Microbiol10.1099/ijs.0.63646-02005Bacterial Typing Techniques, Base Composition, Cellulomonas/*classification/genetics/growth & development/metabolism, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Geologic Sediments/*microbiology, Hydrogen-Ion Concentration, Kenya, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Sodium ChlorideGenetics
Phylogeny19060084Cellulomonas aerilata sp. nov., isolated from an air sample.Lee CM, Weon HY, Hong SB, Jeon YA, Schumann P, Kroppenstedt RM, Kwon SW, Stackebrandt EInt J Syst Evol Microbiol10.1099/ijs.0.2008/002253-02008*Air Microbiology, Cellulomonas/chemistry/*classification/genetics/*physiology, Fatty Acids/analysis, Korea, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Species SpecificityGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8543Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20118)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20118
18621Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20118.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40379Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/13885
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71906Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13961.1StrainInfo: A central database for resolving microbial strain identifiers
117234Curators of the CIPCollection of Institut Pasteur (CIP 102222)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102222