Strain identifier

BacDive ID: 2350

Type strain: Yes

Species: Cellulomonas biazotea

Strain Designation: 127, NRS-127

Strain history: CIP <- 1982, ATCC <- N.R. Smith: strain NRS-127

NCBI tax ID(s): 1709 (species)

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General

@ref: 8537

BacDive-ID: 2350

DSM-Number: 20112

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Cellulomonas biazotea 127 is an aerobe, spore-forming, mesophilic bacterium of the family Cellulomonadaceae.

NCBI tax id

  • NCBI tax id: 1709
  • Matching level: species

strain history

@refhistory
8537<- ATCC <- N.R. Smith, 127
67770K. Suzuki CNF 024 <-- AJ 1569 <-- ATCC 486 <-- N. R. Smith NRS 127.
116800CIP <- 1982, ATCC <- N.R. Smith: strain NRS-127

doi: 10.13145/bacdive2350.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Cellulomonas
  • species: Cellulomonas biazotea
  • full scientific name: Cellulomonas biazotea (Kellerman et al. 1913) Bergey et al. 1923 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus biazoteus

@ref: 8537

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Cellulomonas

species: Cellulomonas biazotea

full scientific name: Cellulomonas biazotea (Kellerman et al. 1913) Bergey et al. 1923

strain designation: 127, NRS-127

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

@refcolony colorincubation periodmedium used
18418Ivory (1014)10-14 daysISP 2
18418Lemon yellow (1012)10-14 daysISP 3
18418Light ivory (1015)10-14 daysISP 4
1841810-14 daysISP 6

multicellular morphology

@refforms multicellular complexmedium name
18418noISP 2
18418noISP 3
18418noISP 4
18418noISP 6

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18418ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18418ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18418ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18418ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
8537CORYNEBACTERIUM AGAR (DSMZ Medium 53)yesName: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/53
37733MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116800CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
18418positiveoptimum28mesophilic
8537positivegrowth30mesophilic
37733positivegrowth30mesophilic
61575positivegrowth30-37mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 61575
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481yes94
69480no99.932

halophily

  • @ref: 18418
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

murein

  • @ref: 8537
  • murein short key: A21.05
  • type: A4ß L-Orn-D-Glu

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1841817234glucose+
1841822599arabinose-
1841817992sucrose-
1841829864mannitol-
1841828757fructose-
1841816634raffinose+
1841862968cellulose-
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
6837916199urea-hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose+fermentation
6837917306maltose+fermentation
6837917716lactose+fermentation
6837917992sucrose+fermentation

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68379catalase+1.11.1.6
68379urease-3.5.1.5
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379pyrrolidonyl arylamidase+3.4.19.3
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
18418--+-++++--+++-++++-
61575++++-++++--+++-++++-+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18418+-+-++++--+++-+++--
61575-+--+----++++-+++---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18418+--------+-

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
37733Logan, UTUSAUSANorth America
61575UT,Logan,Greenville Agr.Exp.FarmUSAUSANorth AmericaSoil
67770Logan, UTUSAUSANorth AmericaSoil from Greenville Agr. Exp. Farm
116800Logan, UtahUnited States of AmericaUSANorth AmericaEnvironment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_3465.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_432;97_1685;98_2660;99_3465&stattab=map
  • Last taxonomy: Cellulomonas
  • 16S sequence: X79462
  • Sequence Identity:
  • Total samples: 959
  • soil counts: 529
  • aquatic counts: 122
  • animal counts: 233
  • plant counts: 75

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
184181
85371Risk group (German classification)
1168001Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218C.biazotea DSM 20112 16S rDNAX794621484ena1709
20218C.biazotea 16S rRNA geneX838021452ena1709

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulomonas biazotea NBRC12680GCA_004306155scaffoldncbi1709
66792Cellulomonas biazotea strain NBRC126801709.6wgspatric1709
66792Cellulomonas biazotea NBRC126802865143354draftimg1709

GC content

@refGC-contentmethod
853772.2
6777072.2thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes94no
motileyes83.266no
flagellatedno91.688no
gram-positiveyes91.858no
anaerobicno98.968yes
aerobicyes89.985no
halophileno93.833no
spore-formingno85.633no
glucose-utilyes88.257yes
thermophileno98.911yes
glucose-fermentno63.985yes

External links

@ref: 8537

culture collection no.: DSM 20112, ATCC 486, NCDO 1654, NCIB 8077, CCUG 58054, JCM 1340, LMG 16695, CIP 82.11, NCIMB 8077, BCRC 14864, CFBP 4269, CGMCC 1.1899, IAM 12106, IFM 10509, IFO 12680, IMET 10473, KCCM 40760, KCTC 1370, NBRC 12680, NCAIM B.01385, VKM Ac-1410, NCTC 10823

straininfo link

  • @ref: 71905
  • straininfo: 8217

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
6245065Structure of the principal carotenoid pigment of Cellulomonas biazotea.Weeks OB, Montes AR, Andrewes AGJ Bacteriol10.1128/jb.141.3.1272-1278.19801980Actinomycetales/*analysis, Carotenoids/*analysis, Chemical Phenomena, Chemistry, Magnetic Resonance Spectroscopy, Mass Spectrometry, Protons, Spectrophotometry, Infrared
18676473Cellulomonas chitinilytica sp. nov., a chitinolytic bacterium isolated from cattle-farm compost.Yoon MH, Ten LN, Im WT, Lee STInt J Syst Evol Microbiol10.1099/ijs.0.64768-02008*Animal Husbandry, Animals, Bacterial Typing Techniques, Base Composition, Cattle, Cellulomonas/*classification/genetics/isolation & purification/physiology, Chitin/*metabolism, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species SpecificityPhylogenyGenetics
19060084Cellulomonas aerilata sp. nov., isolated from an air sample.Lee CM, Weon HY, Hong SB, Jeon YA, Schumann P, Kroppenstedt RM, Kwon SW, Stackebrandt EInt J Syst Evol Microbiol10.1099/ijs.0.2008/002253-02008*Air Microbiology, Cellulomonas/chemistry/*classification/genetics/*physiology, Fatty Acids/analysis, Korea, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Species SpecificityPhylogenyGenetics
20729313Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond.Rusznyak A, Toth EM, Schumann P, Sproer C, Makk J, Szabo G, Vladar P, Marialigeti K, Borsodi AKInt J Syst Evol Microbiol10.1099/ijs.0.022608-02010Bacterial Typing Techniques, Base Composition, Cellulomonas/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hungary, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, Poaceae/*microbiology, Ponds, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryPhylogenyGenetics
31218500Cellulomonas aurantiaca sp. nov., isolated from a soil sample from a tangerine field.Kim SK, Kook M, Yan ZF, Trinh H, Zheng SD, Yang JE, Park SY, Yi THAntonie Van Leeuwenhoek10.1007/s10482-019-01288-22019Cellulomonas/*classification/genetics/*isolation & purification, *Citrus, Genome, Bacterial, Genomics/methods, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Soil, *Soil MicrobiologyPhylogenyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8537Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20112)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20112
18418Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20112.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37733Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11279
61575Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 58054)https://www.ccug.se/strain?id=58054
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71905Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8217.1StrainInfo: A central database for resolving microbial strain identifiers
116800Curators of the CIPCollection of Institut Pasteur (CIP 82.11)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2082.11