Strain identifier
BacDive ID: 23455
Type strain:
Species: Dissulfuribacter thermophilus
Strain Designation: S69
Strain history: <- A. Slobodkin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; S69 <- A. Slobodkin {2010}
NCBI tax ID(s): 1156395 (species)
General
@ref: 19341
BacDive-ID: 23455
DSM-Number: 25762
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, motile, rod-shaped
description: Dissulfuribacter thermophilus S69 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from deep-sea hydrothermal chimney.
NCBI tax id
- NCBI tax id: 1156395
- Matching level: species
strain history
- @ref: 19341
- history: <- A. Slobodkin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; S69 <- A. Slobodkin {2010}
doi: 10.13145/bacdive23455.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Dissulfuribacterales
- family: Dissulfuribacteraceae
- genus: Dissulfuribacter
- species: Dissulfuribacter thermophilus
- full scientific name: Dissulfuribacter thermophilus Slobodkin et al. 2013
@ref: 19341
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Deltaproteobacteria, not assigned to order
family: Dissulfuribacteraceae
genus: Dissulfuribacter
species: Dissulfuribacter thermophilus
full scientific name: Dissulfuribacter thermophilus Slobodkin et al. 2013
strain designation: S69
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
30893 | negative | 1.75 µm | 0.5 µm | rod-shaped | yes | |
69480 | yes | 92.821 | ||||
69480 | negative | 99.996 |
pigmentation
- @ref: 30893
- production: yes
Culture and growth conditions
culture medium
- @ref: 19341
- name: THERMOSULFURIMONAS MEDIUM (DSMZ Medium 1210a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1210a
- composition: Name: THERMOSULFURIMONAS MEDIUM (DSMZ Medium 1210a) Composition: NaCl 17.9462 g/l MgCl2 x 6 H2O 3.98804 g/l Na2S2O3 x 5 H2O 3.48953 g/l Na-acetate 1.49551 g/l Na2CO3 0.997009 g/l (NH4)2SO4 0.498504 g/l KCl 0.329013 g/l CaCl2 x 2 H2O 0.329013 g/l KH2PO4 0.329013 g/l Yeast extract 0.199402 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
19341 | positive | growth | 60 | thermophilic | |
30893 | positive | growth | 28-70 | ||
30893 | positive | optimum | 61 | thermophilic | |
69480 | thermophilic | 100 |
culture pH
@ref | ability | type | pH |
---|---|---|---|
30893 | positive | growth | 5.6-7.9 |
30893 | positive | optimum | 6.8 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
19341 | anaerobe | |
30893 | anaerobe | |
69480 | anaerobe | 99.719 |
spore formation
@ref | spore formation | confidence |
---|---|---|
30893 | no | |
69481 | no | 99 |
69480 | no | 99.929 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
30893 | NaCl | positive | growth | 0.9-5.0 % |
30893 | NaCl | positive | optimum | 2.25 % |
Isolation, sampling and environmental information
isolation
- @ref: 19341
- sample type: deep-sea hydrothermal chimney
- geographic location: Pacific Ocean, Mariner hydrothermal field on the East Lau Spreading Center and Valu Fa Ridge
- country: Tonga
- origin.country: TON
- continent: Australia and Oceania
- latitude: -22.1803
- longitude: -176.602
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Hydrothermal vent |
#Environmental | #Aquatic | #Marine |
#Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_4547.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_474;96_2270;97_2751;98_3417;99_4547&stattab=map
- Last taxonomy: Dissulfuribacter thermophilus subclade
- 16S sequence: JQ414031
- Sequence Identity:
- Total samples: 314
- soil counts: 27
- aquatic counts: 272
- animal counts: 11
- plant counts: 4
Safety information
risk assessment
- @ref: 19341
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19341
- description: Dissulfuribacter thermophilus strain S69 16S ribosomal RNA gene, partial sequence
- accession: JQ414031
- length: 1502
- database: ena
- NCBI tax ID: 1156395
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Dissulfuribacter thermophilus S69 | GCA_001687335 | contig | ncbi | 1156395 |
66792 | Dissulfuribacter thermophilus strain S69 | 1156395.6 | wgs | patric | 1156395 |
66792 | Dissulfuribacter thermophilus S69 | 2724679641 | draft | img | 1156395 |
GC content
@ref | GC-content | method |
---|---|---|
19341 | 40.5 | thermal denaturation, midpoint method (Tm) |
30893 | 40.5 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 99 | no |
motile | yes | 90.105 | no |
gram-positive | no | 99.645 | no |
anaerobic | yes | 99.999 | yes |
aerobic | no | 99.407 | yes |
halophile | no | 78.77 | no |
spore-forming | no | 95.504 | no |
flagellated | no | 78.476 | no |
thermophile | yes | 99.188 | no |
glucose-util | no | 70.432 | no |
glucose-ferment | no | 81.153 | no |
External links
@ref: 19341
culture collection no.: DSM 25762, VKM B-2760
straininfo link
- @ref: 87604
- straininfo: 398252
literature
- topic: Phylogeny
- Pubmed-ID: 23024145
- title: Dissulfuribacter thermophilus gen. nov., sp. nov., a thermophilic, autotrophic, sulfur-disproportionating, deeply branching deltaproteobacterium from a deep-sea hydrothermal vent.
- authors: Slobodkin AI, Reysenbach AL, Slobodkina GB, Kolganova TV, Kostrikina NA, Bonch-Osmolovskaya EA
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.046938-0
- year: 2012
- mesh: Bacterial Typing Techniques, Base Composition, Chemoautotrophic Growth, DNA, Bacterial/genetics, Deltaproteobacteria/*classification/genetics/isolation & purification, Hydrothermal Vents/*microbiology, Molecular Sequence Data, Pacific Ocean, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Sulfur/*metabolism
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
---|---|---|---|---|---|---|---|
19341 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25762) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25762 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
30893 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27223 | 28776041 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
87604 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID398252.1 | StrainInfo: A central database for resolving microbial strain identifiers |