Strain identifier

BacDive ID: 23455

Type strain: Yes

Species: Dissulfuribacter thermophilus

Strain Designation: S69

Strain history: <- A. Slobodkin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; S69 <- A. Slobodkin {2010}

NCBI tax ID(s): 1156395 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 19341

BacDive-ID: 23455

DSM-Number: 25762

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, motile, rod-shaped

description: Dissulfuribacter thermophilus S69 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from deep-sea hydrothermal chimney.

NCBI tax id

  • NCBI tax id: 1156395
  • Matching level: species

strain history

  • @ref: 19341
  • history: <- A. Slobodkin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; S69 <- A. Slobodkin {2010}

doi: 10.13145/bacdive23455.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Dissulfuribacterales
  • family: Dissulfuribacteraceae
  • genus: Dissulfuribacter
  • species: Dissulfuribacter thermophilus
  • full scientific name: Dissulfuribacter thermophilus Slobodkin et al. 2013

@ref: 19341

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Deltaproteobacteria, not assigned to order

family: Dissulfuribacteraceae

genus: Dissulfuribacter

species: Dissulfuribacter thermophilus

full scientific name: Dissulfuribacter thermophilus Slobodkin et al. 2013

strain designation: S69

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
30893negative1.75 µm0.5 µmrod-shapedyes
69480yes92.821
69480negative99.996

pigmentation

  • @ref: 30893
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 19341
  • name: THERMOSULFURIMONAS MEDIUM (DSMZ Medium 1210a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1210a
  • composition: Name: THERMOSULFURIMONAS MEDIUM (DSMZ Medium 1210a) Composition: NaCl 17.9462 g/l MgCl2 x 6 H2O 3.98804 g/l Na2S2O3 x 5 H2O 3.48953 g/l Na-acetate 1.49551 g/l Na2CO3 0.997009 g/l (NH4)2SO4 0.498504 g/l KCl 0.329013 g/l CaCl2 x 2 H2O 0.329013 g/l KH2PO4 0.329013 g/l Yeast extract 0.199402 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
19341positivegrowth60thermophilic
30893positivegrowth28-70
30893positiveoptimum61thermophilic
69480thermophilic100

culture pH

@refabilitytypepH
30893positivegrowth5.6-7.9
30893positiveoptimum6.8

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
19341anaerobe
30893anaerobe
69480anaerobe99.719

spore formation

@refspore formationconfidence
30893no
69481no99
69480no99.929

halophily

@refsaltgrowthtested relationconcentration
30893NaClpositivegrowth0.9-5.0 %
30893NaClpositiveoptimum2.25 %

Isolation, sampling and environmental information

isolation

  • @ref: 19341
  • sample type: deep-sea hydrothermal chimney
  • geographic location: Pacific Ocean, Mariner hydrothermal field on the East Lau Spreading Center and Valu Fa Ridge
  • country: Tonga
  • origin.country: TON
  • continent: Australia and Oceania
  • latitude: -22.1803
  • longitude: -176.602

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Hydrothermal vent
#Environmental#Aquatic#Marine
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4547.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_474;96_2270;97_2751;98_3417;99_4547&stattab=map
  • Last taxonomy: Dissulfuribacter thermophilus subclade
  • 16S sequence: JQ414031
  • Sequence Identity:
  • Total samples: 314
  • soil counts: 27
  • aquatic counts: 272
  • animal counts: 11
  • plant counts: 4

Safety information

risk assessment

  • @ref: 19341
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19341
  • description: Dissulfuribacter thermophilus strain S69 16S ribosomal RNA gene, partial sequence
  • accession: JQ414031
  • length: 1502
  • database: ena
  • NCBI tax ID: 1156395

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Dissulfuribacter thermophilus S69GCA_001687335contigncbi1156395
66792Dissulfuribacter thermophilus strain S691156395.6wgspatric1156395
66792Dissulfuribacter thermophilus S692724679641draftimg1156395

GC content

@refGC-contentmethod
1934140.5thermal denaturation, midpoint method (Tm)
3089340.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileyes90.105no
gram-positiveno99.645no
anaerobicyes99.999yes
aerobicno99.407yes
halophileno78.77no
spore-formingno95.504no
flagellatedno78.476no
thermophileyes99.188no
glucose-utilno70.432no
glucose-fermentno81.153no

External links

@ref: 19341

culture collection no.: DSM 25762, VKM B-2760

straininfo link

  • @ref: 87604
  • straininfo: 398252

literature

  • topic: Phylogeny
  • Pubmed-ID: 23024145
  • title: Dissulfuribacter thermophilus gen. nov., sp. nov., a thermophilic, autotrophic, sulfur-disproportionating, deeply branching deltaproteobacterium from a deep-sea hydrothermal vent.
  • authors: Slobodkin AI, Reysenbach AL, Slobodkina GB, Kolganova TV, Kostrikina NA, Bonch-Osmolovskaya EA
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.046938-0
  • year: 2012
  • mesh: Bacterial Typing Techniques, Base Composition, Chemoautotrophic Growth, DNA, Bacterial/genetics, Deltaproteobacteria/*classification/genetics/isolation & purification, Hydrothermal Vents/*microbiology, Molecular Sequence Data, Pacific Ocean, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Sulfur/*metabolism
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
19341Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25762)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25762
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30893Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2722328776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
87604Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID398252.1StrainInfo: A central database for resolving microbial strain identifiers