Strain identifier

BacDive ID: 23454

Type strain: Yes

Species: Blastocatella fastidiosa

Strain Designation: A2-16, A2_16

Strain history: <- B. Fösel, Leibniz-Institute DSMZ, Braunschweig, Germany; A2_16

NCBI tax ID(s): 1235480 (species)

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General

@ref: 19328

BacDive-ID: 23454

DSM-Number: 25172

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, motile, other

description: Blastocatella fastidiosa A2-16 is a mesophilic, Gram-negative, motile bacterium that was isolated from savanna soil, loamy sand.

NCBI tax id

  • NCBI tax id: 1235480
  • Matching level: species

strain history

  • @ref: 19328
  • history: <- B. Fösel, Leibniz-Institute DSMZ, Braunschweig, Germany; A2_16

doi: 10.13145/bacdive23454.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/acidobacteriota
  • domain: Bacteria
  • phylum: Acidobacteriota
  • class: Blastocatellia
  • order: Blastocatellales
  • family: Blastocatellaceae
  • genus: Blastocatella
  • species: Blastocatella fastidiosa
  • full scientific name: Blastocatella fastidiosa Foesel et al. 2013

@ref: 19328

domain: Bacteria

phylum: Acidobacteria

class: Blastocatellia

order: Blastocatellales

family: Blastocatellaceae

genus: Blastocatella

species: Blastocatella fastidiosa

full scientific name: Blastocatella fastidiosa Foesel et al. 2013 emend. Pascual et al. 2015

strain designation: A2-16, A2_16

type strain: yes

Morphology

cell morphology

  • @ref: 21410
  • gram stain: negative
  • cell length: 10 µm
  • cell width: 0.85 µm
  • cell shape: other
  • motility: yes

colony morphology

@refincubation periodcolony sizecolony colorcolony shape
193288-14 days
214101 mmorange to pink, slighty translucent, smooth, shiny and umbonate with entire marginscircular

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21410SSE/HD1:10 contains the basal medium described above plus 0.25 g/l of yeast extract (Difco), 0.5 g/l of peptone (Difco), and 0.1 g/l of glucose (SigmaAldrich)
19328SSE/HD1:10 (DSMZ Medium 1426)yeshttps://mediadive.dsmz.de/medium/1426Name: SSE/HD 1:10 MEDIUM (DSMZ Medium 1426) Composition: MES 1.95 g/l Peptone 0.5 g/l CaSO4 x 2 H2O 0.4303 g/l MgSO4 x 7 H2O 0.3695 g/l Yeast extract 0.25 g/l Na NO3 0.212 g/l CaCl2 x 2 H2O 0.1469 g/l Ca(NO3)2 x 4 H2O 0.118 g/l MgCl2 x 6 H2O 0.1018 g/l Glucose 0.1 g/l (NH4)2SO4 0.09915 g/l NH4Cl 0.05345 g/l K2SO4 0.0435 g/l FeSO4 x 7 H2O 0.00555 g/l KH2PO4 0.00340213 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Pyridoxine hydrochloride 1e-05 g/l H3BO3 6e-06 g/l Lipoic acid 5e-06 g/l D-Calcium pantothenate 5e-06 g/l Nicotinic acid 5e-06 g/l Riboflavin 5e-06 g/l Thiamine-HCl x 2 H2O 5e-06 g/l p-Aminobenzoic acid 5e-06 g/l Folic acid 2e-06 g/l Biotin 2e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19328positivegrowth28mesophilic
21410positivemaximum40thermophilic
21410positiveminimum14psychrophilic
21410positiveoptimum32mesophilic

culture pH

@refabilitytypepHPH range
21410positivemaximum10alkaliphile
21410positiveminimum4acidophile
21410positiveoptimum6.25

Physiology and metabolism

halophily

  • @ref: 21410
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 1 %

metabolite utilization

@refmetaboliteutilization activityChebi-IDkind of utilization tested
21410casamino acids+
21410casein hydrolysate+
21410protocatechuate+36241
21410cellulose+/-62968
21410starch+/-28017
21410chitin+/-17029
21410esculin+4853
21410gelatin hydrolyzed+
21410arabinose-22599
21410cellobiose-17057
21410fructose-28757
21410fucose-33984
21410galactose-28260
21410glucose-17234
21410lactose-17716
21410maltose-17306
21410mannose-37684
21410melezitose-6731
21410raffinose-16634
21410rhamnose-26546
21410sorbose-27922
21410sucrose-17992
21410trehalose-27082
21410xylose-18222
21410N-acetylglucosamine-59640
21410galactitol-16813
21410mannitol-29864
21410myo-inositol-17268
21410sorbitol-30911
21410malate-25115
21410pyruvate-15361
21410succinate-30031
21410ethanol-16236
21410glycerol-17754
21410peptone+
21410yeast extract+
21410laminarin-6364
21410fermented rumen extract-
214101,2-propandiol-16997
21410methanol-17790
21410propanol-28831
21410erythrose-33946
21410erythrulose-23958
21410glucosamine-5417
21410ribitol-15963
21410arabinitol-22605
21410xylitol-17151
21410alanine-16449
21410arginine-29016
21410aspartate-35391
21410cysteine-15356
21410glutamate-29987
21410glycine-15428
21410histidine-27570
21410hydroxyproline-24741
21410isoleucine-24898
21410leucine-25017
21410lysine-25094
21410methionine-16811
21410ornithine-18257
21410phenylalanine-28044
21410proline-26271
21410serine-17822
21410threonine-26986
21410tryptophan-27897
21410tyrosine-18186
21410valine-27266
21410adipate-17128
21410acetate-30089
21410ascorbate-22651
21410benzoate-16150
21410trimethoxybenzoate-
21410butyrate-17968
21410alpha-hydroxybutyrate-
21410beta-hydroxybutyrate-8295
214104-hydroxybutyrate-16724
21410isobutyrate-48944
21410hexanoate-17120
21410octanoate-25646
21410citrate-16947
21410isocitrate-16087
21410crotonate-35899
21410formate-15740
21410fumarate-29806
21410gluconate-24265
214102-oxogluconate-
21410glucuronate-24297
214102-oxoglutarate-
21410glycolate-29805
21410glyoxylate-36655
21410isovalerate-48942
214104-oxopentanoate-39150
21410lactate-24996
21410malonate-15792
21410nicotinate-32544
21410oxaloacetate-16452
21410propionate-17272
21410tartrate-132950
214102-oxopentanoate-28644
21410butanol-
214101,2-butandiol-
214102,3-butanediol-
21410ethylene glycol-30742
68369malate-25115assimilation
68369adipate-17128assimilation
68369decanoate-27689assimilation
68369gluconate-24265assimilation
68369maltose-17306assimilation
68369N-acetylglucosamine-59640assimilation
68369D-mannitol-16899assimilation
68369D-mannose-16024assimilation
68369L-arabinose-30849assimilation
68369D-glucose-17634assimilation
68369gelatin-5291hydrolysis
68369esculin+4853hydrolysis
68369urea-16199hydrolysis
68369arginine-29016hydrolysis
68369D-glucose-17634fermentation
68369tryptophan-27897energy source
68369nitrate-17632reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
19328catalase+1.11.1.6
19328cytochrome-c oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21410-++++++++-++--+--+--

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
19328-----+--------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateenrichment cultureenrichment culture composition
19328savanna soil, loamy sandErichsfelde (21°36'41.4'' S, 16°52'13.4'' E), 1481 m height above sea levelNamibiaNAMAfrica-21.611516.8704
21410soil sample - sandy savanna soil, loamy sand pH = 5.0 and 6.1, measured in 2mM CaCl2 and in destillied water, respectively2013-00-00soil suspensiondirect plating of a soil suspension [10 mM 2-(N-morpholino)ethanesulfonic acid (MES), pH 5.5]on solidified media containing soil solution equivalent (SSE) [1] with the iron content changed from 5 to 50 Ã?µM and buffered with 10 mM MES at a pH of 5.5. basal medium was supplemented with 0.1 g lâË?â??1 yeast extract (Difco Laboratories Inc.,Detroit, MI), 0.1 g lâË?â??1 of peptone (Difco), 0.1 ml lâË?â??1 ten vitamin mixture [2], and 1 ml lâË?â??1 trace element solution SL 10 [60]. Gellan Gum (

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Sandy
#Environmental#Terrestrial#Soil
#Condition#Acidic

taxonmaps

  • @ref: 69479
  • File name: preview.99_143099.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_737;96_6965;97_22594;98_95024;99_143099&stattab=map
  • Last taxonomy: Blastocatella fastidiosa subclade
  • 16S sequence: JQ309130
  • Sequence Identity:
  • Total samples: 134
  • soil counts: 109
  • aquatic counts: 12
  • animal counts: 12
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
214101
193281Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19328
  • description: Blastocatella fastidiosa strain A2-16 16S ribosomal RNA gene, partial sequence
  • accession: JQ309130
  • length: 1445
  • database: ena
  • NCBI tax ID: 1235480

GC content

@refGC-contentmethod
1932846.5high performance liquid chromatography (HPLC)
2141046.5

External links

@ref: 19328

culture collection no.: DSM 25172, LMG 26944

straininfo link

  • @ref: 87603
  • straininfo: 379775

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23266188Blastocatella fastidiosa gen. nov., sp. nov., isolated from semiarid savanna soil - the first described species of Acidobacteria subdivision 4.Foesel BU, Rohde M, Overmann JSyst Appl Microbiol10.1016/j.syapm.2012.11.0022012Acidobacteria/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Desert Climate, Fatty Acids/analysis, Heterotrophic Processes, Locomotion, Microscopy, Molecular Sequence Data, Organic Chemicals/metabolism, Phylogeny, Pigments, Biological/metabolism, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny24573163Aridibacter famidurans gen. nov., sp. nov. and Aridibacter kavangonensis sp. nov., two novel members of subdivision 4 of the Acidobacteria isolated from semiarid savannah soil.Huber KJ, Wust PK, Rohde M, Overmann J, Foesel BUInt J Syst Evol Microbiol10.1099/ijs.0.060236-02014Acidobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Namibia, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
19328Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25172)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25172
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
21410Foesel B. U. , Rohde M. , Overmann J.10.1016/j.syapm.2012.11.002Blastocatella fastidiosa gen. nov., sp. nov., isolated from semiarid savanna soil - the first described species of Acidobacteria subdivision 4.Syst Appl Microbiol. 36: 82-89 201323266188
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87603Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID379775.1StrainInfo: A central database for resolving microbial strain identifiers