Strain identifier

BacDive ID: 23435

Type strain: Yes

Species: Caldinitratiruptor microaerophilus

Strain Designation: 62N, CA62N

Strain history: M.-L. Fardeau CA62N.

NCBI tax ID(s): 671077 (species)

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General

@ref: 16452

BacDive-ID: 23435

DSM-Number: 22660

keywords: 16S sequence, Bacteria, anaerobe, thermophilic

description: Caldinitratiruptor microaerophilus 62N is an anaerobe, thermophilic bacterium that was isolated from hot spring.

NCBI tax id

  • NCBI tax id: 671077
  • Matching level: species

strain history

@refhistory
16452<- M.-L. Fardeau, Lab. Microbiol. de l'IRD/ESIL, Univ. de Provence et de la Méditerranée, Marseille, France; CA62N <- M.-L. Fardeau {2006}
67770M.-L. Fardeau CA62N.

doi: 10.13145/bacdive23435.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacillota, not assigned to class
  • order: Bacillota, not assigned to order
  • family: Bacillota, not assigned to family
  • genus: Caldinitratiruptor
  • species: Caldinitratiruptor microaerophilus
  • full scientific name: Caldinitratiruptor microaerophilus Fardeau et al. 2010

@ref: 16452

domain: Bacteria

phylum: Firmicutes

class: Firmicutes, not assigned to class

order: Firmicutes, not assigned to order

family: Firmicutes, not assigned to family

genus: Caldinitratiruptor

species: Caldinitratiruptor microaerophilus

full scientific name: Caldinitratiruptor microaerophilus Fardeau et al. 2010

strain designation: 62N, CA62N

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 16452
  • name: CALDINITRATIRUPTOR MEDIUM (DSMZ Medium 1251)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1251
  • composition: Name: CALDINITRATIRUPTOR MEDIUM (DSMZ Medium 1251) Composition: D-Glucose 3.5 g/l NaNO3 1.5 g/l Na2CO3 1.0 g/l Yeast extract 1.0 g/l NH4Cl 1.0 g/l MgCl2 x 6 H2O 0.5 g/l K2HPO4 0.3 g/l KH2PO4 0.3 g/l KCl 0.1 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16452positivegrowth65thermophilic
67770positivegrowth60thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 16452
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
16452hot springFranceFRAEurope
67770Hot spring at Chaudes-Aigues in the Massif CentralFranceFRAEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_118174.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10726;96_46727;97_60010;98_79742;99_118174&stattab=map
  • Last taxonomy: Caldinitratiruptor microaerophilus subclade
  • 16S sequence: GQ405534
  • Sequence Identity:
  • Total samples: 402
  • soil counts: 157
  • aquatic counts: 97
  • animal counts: 124
  • plant counts: 24

Sequence information

16S sequences

  • @ref: 16452
  • description: Caldinitratiruptor microaerophilus strain CA62N 16S ribosomal RNA gene, partial sequence
  • accession: GQ405534
  • length: 1524
  • database: ena
  • NCBI tax ID: 671077

GC content

  • @ref: 16452
  • GC-content: 70.3
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 16452

culture collection no.: DSM 22660, JCM 16183

literature

  • topic: Phylogeny
  • Pubmed-ID: 20165893
  • title: Caldinitratiruptor microaerophilus, gen. nov., sp. nov. isolated from a French hot spring (Chaudes-Aigues, Massif Central): a novel cultivated facultative microaerophilic anaerobic thermophile pertaining to the Symbiobacterium branch within the Firmicutes.
  • authors: Fardeau ML, Barsotti V, Cayol JL, Guasco S, Michotey V, Joseph M, Bonin P, Ollivier B
  • journal: Extremophiles
  • DOI: 10.1007/s00792-010-0302-y
  • year: 2010
  • mesh: Bacteria, Anaerobic/*genetics, Base Composition/genetics, Ecosystem, Evolution, Molecular, France, Geologic Sediments, Hot Springs/*microbiology, Hot Temperature, Hydrogen-Ion Concentration, Microscopy, Electron/methods, Phenotype, Phylogeny, Polymerase Chain Reaction, Sequence Analysis, DNA, Temperature
  • topic2: Stress

Reference

@idauthorscataloguedoi/urltitle
16452Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22660)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22660
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta