Strain identifier

BacDive ID: 23318

Type strain: Yes

Species: Streptomyces chlorus

Strain Designation: BK125

Strain history: CGMCC 4.5798 <-- B.-Y. Kim et al. BK125.

NCBI tax ID(s): 887452 (species)

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General

@ref: 19320

BacDive-ID: 23318

DSM-Number: 42079

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Streptomyces chlorus BK125 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from hay meadow soil.

NCBI tax id

  • NCBI tax id: 887452
  • Matching level: species

strain history

@refhistory
19320<- S.-W. Kwon, KACC; KACC 20902
67770CGMCC 4.5798 <-- B.-Y. Kim et al. BK125.

doi: 10.13145/bacdive23318.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces chlorus
  • full scientific name: Streptomyces chlorus Kim et al. 2013

@ref: 19320

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces chlorus

full scientific name: Streptomyces chlorus Kim et al. 2013

strain designation: BK125

type strain: yes

Morphology

cell morphology

  • @ref: 30851
  • gram stain: positive
  • cell length: 0.7 µm
  • cell width: 0.6 µm
  • cell shape: rod-shaped

colony morphology

@refcolony colormedium used
69341Brown beige (1011), ivory (1014)ISP 6
69341Beige (1001), brown beige (1011)ISP 7
69341Ivory (1014)ISP 4
69341Ivory (1014), brown beige (1011)ISP 5
69341Ivory (1014), khaki grey (7008)ISP 3
69341Ivory (1014), ochre yellow (1024)suter without tyrosine
69341Maize yellow (1006), sand yellow (1002)suter with tyrosine
69341Sand yellow (1002)ISP 2

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69341noAerial myceliumISP 2
69341noAerial myceliumISP 3
69341noAerial myceliumISP 4
69341noAerial myceliumISP 5
69341noAerial myceliumISP 6
69341noAerial myceliumISP 7
69341yesAerial myceliumsuter with tyrosineOyster white (1013)
69341yesAerial myceliumsuter without tyrosinePebble grey (7032)

pigmentation

@refproductionnamecolor
30851yes
69341yesMelanin
69341yessoluble pigmentBrown beige (1011), sand yellow (1002)

multimedia

@refmultimedia contentcaptionintellectual property rights
19320https://www.dsmz.de/microorganisms/photos/DSM_42079.jpgMedium 65 28°C© Leibniz-Institut DSMZ
69341DSM_42079_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69341DSM_42079_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
19320ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf
19320STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf
19320GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperature
19320positivegrowth28
30851positivegrowth10-42
30851positiveoptimum30
67770positivegrowth28

culture pH

@refabilitytypepHPH range
30851positivegrowth05-09alkaliphile
30851positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30851
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30851
  • spore formation: yes

halophily

  • @ref: 69341
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

observation

@refobservation
30851aggregates in chains
67770quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3085118403L-arabitol+carbon source
3085116947citrate+carbon source
3085124996lactate+carbon source
3085125115malate+carbon source
3085117306maltose+carbon source
3085128053melibiose+carbon source
3085117272propionate+carbon source
3085115361pyruvate+carbon source
3085130911sorbitol+carbon source
3085130031succinate+carbon source
308514853esculin+hydrolysis
3085117632nitrate+reduction
6837928087glycogen-fermentation
6837917992sucrose-fermentation
6837917716lactose-fermentation
6837917306maltose-fermentation
6837917634D-glucose+fermentation
6837965327D-xylose-fermentation
683795291gelatin+hydrolysis
6837916199urea-hydrolysis
6837917632nitrate+reduction
6934122599arabinose+growth
6934162968cellulose-growth
6934128757fructose+growth
6934117234glucose+growth
6934117268inositol+growth
6934137684mannose+growth
6934116634raffinose-growth
6934126546rhamnose+growth
6934117992sucrose+growth
6934118222xylose+growth
6837916899D-mannitol-fermentation

enzymes

@refvalueactivityec
30851catalase+1.11.1.6
30851gelatinase+
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382leucine arylamidase+3.4.11.1
68382trypsin-3.4.21.4
68382lipase (C 14)-
68382esterase (C 4)+
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382alkaline phosphatase+3.1.3.1
68379gelatinase+
68379urease-3.5.1.5
68379beta-galactosidase-3.2.1.23
68379pyrrolidonyl arylamidase-3.4.19.3

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69341++/--+--+-+/--+++/-------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69341+++/--++/-+/---++---+-+/---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
19320hay meadow soilNorthumberland, Cockle Park Experimental Farm, Palace Leas hay meadow plot 6United KingdomGBREurope
67770Soil from Palace Leas hay meadow plot 6 at Cockle Park Experimental FarmNorthumberlandUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Meadow
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 19320
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19320
  • description: Streptomyces chlorus partial 16S rRNA gene, strain BK125
  • accession: FR692094
  • length: 1460
  • database: nuccore
  • NCBI tax ID: 887452

GC content

@refGC-contentmethod
1932069.2fluorimetric
3085169.2
6777069.2thermal denaturation, midpoint method (Tm)

External links

@ref: 19320

culture collection no.: DSM 42079, CGMCC 4.5798, KACC 20902, JCM 19672

straininfo link

  • @ref: 87536
  • straininfo: 405319

literature

  • topic: Phylogeny
  • Pubmed-ID: 22922536
  • title: Streptomyces chlorus sp. nov. and Streptomyces viridis sp. nov., isolated from soil.
  • authors: Kim BY, Rong X, Zucchi TD, Huang Y, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.045906-0
  • year: 2012
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
19320Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42079)https://www.dsmz.de/collection/catalogue/details/culture/DSM-42079
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30851Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2718128776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69341Wink, J.https://cdn.dsmz.de/wink/DSM%2042079.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
87536Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405319.1StrainInfo: A central database for resolving microbial strain identifiers