Strain identifier

BacDive ID: 23300

Type strain: Yes

Species: Streptococcus hongkongensis

Strain Designation: HKU30

Strain history: <- S. K. P. Lau, Univ. Hong Kong, Dept. of Microbiolo.; HKU30

NCBI tax ID(s): 910939 (species)

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General

@ref: 19366

BacDive-ID: 23300

DSM-Number: 26014

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, human pathogen

description: Streptococcus hongkongensis HKU30 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from infected tissue of a patient with wound infection after puncture by a fish fin.

NCBI tax id

  • NCBI tax id: 910939
  • Matching level: species

strain history

  • @ref: 19366
  • history: <- S. K. P. Lau, Univ. Hong Kong, Dept. of Microbiolo.; HKU30

doi: 10.13145/bacdive23300.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus hongkongensis
  • full scientific name: Streptococcus hongkongensis Lau et al. 2013

@ref: 19366

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus hongkongensis

full scientific name: Streptococcus hongkongensis Lau et al. 2013

strain designation: HKU30

type strain: yes

Morphology

cell morphology

  • @ref: 30815
  • gram stain: positive
  • cell length: 0.75 µm
  • cell width: 0.75 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 19366
  • type of hemolysis: gamma
  • incubation period: 1-2 days

pigmentation

  • @ref: 30815
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
19366COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
19366TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19366positivegrowth37mesophilic
30815positiveoptimum37mesophilic
63338positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
19366microaerophile
30815facultative anaerobe
63338microaerophile

spore formation

  • @ref: 30815
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3081528260galactose+carbon source
3081528087glycogen+carbon source
3081517716lactose+carbon source
3081517306maltose+carbon source
3081529864mannitol+carbon source
3081537684mannose+carbon source
30815506227N-acetylglucosamine+carbon source
3081533942ribose+carbon source
3081517814salicin+carbon source
3081517992sucrose+carbon source
3081527082trehalose+carbon source
308154853esculin+hydrolysis
6837028087glycogen+builds acid from
6837028017starch+builds acid from
6837016634raffinose-builds acid from
6837015443inulin-builds acid from
6837027082trehalose+builds acid from
6837017716lactose+builds acid from
6837017924D-sorbitol-builds acid from
6837016899D-mannitol+builds acid from
6837030849L-arabinose-builds acid from
6837016988D-ribose+builds acid from
6837029016arginine+hydrolysis
683704853esculin+hydrolysis
68370606565hippurate+hydrolysis
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838127941pullulan+builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6837015688acetoinno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6838035581indole-
6837015688acetoin-

enzymes

@refvalueactivityec
19366catalase+1.11.1.6
19366cytochrome-c oxidase-1.9.3.1
30815alkaline phosphatase+3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase+3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5
68370arginine dihydrolase+3.5.3.6
68370leucine arylamidase+3.4.11.1
68370alkaline phosphatase+3.1.3.1
68370beta-galactosidase+3.2.1.23
68370beta-glucuronidase-3.2.1.31
68370alpha-galactosidase-3.2.1.22
68370pyrrolidonyl arylamidase+3.4.19.3
68370beta-glucosidase+3.2.1.21

API 20STR

@refVPHIPESCPYRAalpha GALbeta GURbeta GALPALLAPADHRIBARAMANSORLACTREINURAFAMDGLYGbeta HEM
63338-+++--+++++-+-++--++-

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
63338-+-+-++--++-----++-++++++++-+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
19366+++--+++-++-+----+++++--++--+-+-
19366+++--+++-++-+---++++++-+++--+-+-
63338+++--+++-++-+----++-+---++--+-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
19366infected tissue of a patient with wound infection after puncture by a fish finHong KongChinaCHNAsia
63338Wound,right thumb,punctured by a fish fin2010

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Host#Fishes
#Host Body-Site#Other#Wound

Safety information

risk assessment

  • @ref: 19366
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19366
  • description: Streptococcus hongkongensis strain HKU30 16S ribosomal RNA gene, partial sequence
  • accession: HQ335006
  • length: 1414
  • database: ena
  • NCBI tax ID: 910939

GC content

@refGC-contentmethod
1936635.6thermal denaturation, midpoint method (Tm)
3081535.6

External links

@ref: 19366

culture collection no.: CCUG 66491, DSM 26014, CECT 8154

straininfo link

  • @ref: 87519
  • straininfo: 390299

literature

  • topic: Phylogeny
  • Pubmed-ID: 23264498
  • title: Streptococcus hongkongensis sp. nov., isolated from a patient with an infected puncture wound and from a marine flatfish.
  • authors: Lau SKP, Curreem SOT, Lin CCN, Fung AMY, Yuen KY, Woo PCY
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.045120-0
  • year: 2012
  • mesh: Adult, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Flatfishes/*microbiology, Genes, Bacterial, Humans, Male, Molecular Sequence Data, Nucleic Acid Hybridization, Occupational Exposure, *Phylogeny, *Punctures, RNA, Ribosomal, 16S/genetics, Streptococcal Infections/*microbiology, Streptococcus/*classification/genetics/isolation & purification
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
19366Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26014)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26014
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30815Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2714628776041
63338Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 66491)https://www.ccug.se/strain?id=66491
68370Automatically annotated from API 20STR
68380Automatically annotated from API rID32A
68381Automatically annotated from API rID32STR
87519Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID390299.1StrainInfo: A central database for resolving microbial strain identifiers